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A hemicentric inversion in the maize line knobless Tama flint created two sites of centromeric elements and moved the kinetochore-forming region

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Abstract

A maize line, knobless Tama flint (KTF), was found to contain a version of chromosome 8 with two spatially distinct regions of centromeric elements, one at the original genetic position and the other at a novel location on the long arm. The new site of centromeric elements functions as the kinetochore-forming region resulting in a change of arm length ratio. Examination of fluorescence in situ hybridization markers on chromosome 8 revealed an inversion between the two centromere sites relative to standard maize lines, indicating that this chromosome 8 resulted from a hemicentric inversion with one breakpoint approximately 20 centi-McClintocks (cMc) on the long arm (20% of the total arm length from the centromere) and the other in the original cluster of centromere repeats. This inversion moved the kinetochore-forming region but left the remainder of the centromere repeats. In a hybrid between a standard line (Mo17) and KTF, both chromosome 8 homologues were completely synapsed at pachytene despite the inversion. Although the homologous centromeres were not paired, they were always correctly oriented at anaphase and migrated to opposite poles. Additionally, recombination on 8L was severely repressed in the hybrid.

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Acknowledgment

We thank Marty Sachs (Maize Genetics COOP Stock Center) and Mark Millard (North Central Regional Plant Introduction Station) for their help in tracking the pedigree of the KTF line. This work was supported by grants from the National Science Foundation Plant Genome Initiative (DBI0421671 and DBI0423898).

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Authors and Affiliations

  1. Division of Biological Sciences, University of Missouri-Columbia, 117 Tucker Hall, Columbia, MO, 65211, USA

    Jonathan C. Lamb & James A. Birchler

  2. Department of Agriculture, Truman State University, Kirksville, MO, 63501, USA

    Julie M. Meyer

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  1. Jonathan C. Lamb
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  2. Julie M. Meyer
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  3. James A. Birchler
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Correspondence to James A. Birchler.

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Communicated by I. Schubert

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Lamb, J.C., Meyer, J.M. & Birchler, J.A. A hemicentric inversion in the maize line knobless Tama flint created two sites of centromeric elements and moved the kinetochore-forming region. Chromosoma 116, 237–247 (2007). https://doi.org/10.1007/s00412-007-0096-6

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  • DOI: https://doi.org/10.1007/s00412-007-0096-6

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