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Abundance and chromosomal distribution of six Drosophila buzzatii transposons: BuT1, BuT2, BuT3, BuT4, BuT5, and BuT6

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Abstract

The abundance and chromosomal distribution of six class-II transposable elements (TEs) of Drosophila buzzatii have been analyzed by Southern blotting and in situ hybridization. These six transposons had been previously found at the breakpoints of inversions 2j and 2q 7 of D. buzzatii. These two polymorphic inversions were generated by an ectopic recombination event between two copies of Galileo, a Foldback element. The four breakpoints became hotspots for TE insertions after the generation of the inversion and the transposons analyzed in this work are considered to be secondary invaders of these regions. Insertions of the six transposons are present in the euchromatin but show an increased density in the pericentromeric euchromatin–heterochromatin transition region and the dot chromosome. They are also more abundant in the inverted segments of chromosome 2 rearrangements. We further observed that the accumulation of TE insertions varies between elements and is correlated between dot, proximal regions, and inverted segments. These observations fully agree with previous data in Drosophila melanogaster and support recombination rate as the chief force explaining the chromosomal distribution of TEs.

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Acknowledgement

This work was supported by grant BMC2002-01708 from the Dirección General de Enseñanza Superior e Investigación Científica (Ministerio de Educación y Cultura, Spain), which was awarded to A.R.

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Author notes

  1. Ferran Casals

    Present address: Unitat de Biologia Evolutiva, Facultat de Ciències de la Salut i de la Vida, Universitat Pompeu Fabra, 08003, Barcelona, Spain

  2. Josefa González

    Present address: Department of Biological Sciences, Stanford University, Stanford, CA, 94305, USA

Authors and Affiliations

  1. Departament de Genètica i de Microbiologia, Universitat Autònoma de Barcelona, 08193, Bellaterra, Barcelona, Spain

    Ferran Casals, Josefa González & Alfredo Ruiz

Authors
  1. Ferran Casals
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  2. Josefa González
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  3. Alfredo Ruiz
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Corresponding author

Correspondence to Alfredo Ruiz.

Additional information

Communicated by C. Lehner

Sequence data from this article have been deposited in the EMBL/GenBank Data Libraries under accession number DQ402469.

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Fig. S1

Southern blot hybridization of (a) BuT1, (b) BuT2, (c) BuT3, (d) BuT4, (e) BuT5 and (f) BuT6 probes to genomic DNA of the following D. buzzatii lines (from left to right): st-1, st-3, st-7, st-10, j-2, j-9, j-19, j-23, j-24, jq7-1, jq7-4, jz3-6, jz3-7, y3-1 and s-1 (JPEG 39 kb)

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Casals, F., González, J. & Ruiz, A. Abundance and chromosomal distribution of six Drosophila buzzatii transposons: BuT1, BuT2, BuT3, BuT4, BuT5, and BuT6 . Chromosoma 115, 403–412 (2006). https://doi.org/10.1007/s00412-006-0071-7

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