Abstract
Studies of the meiosis of diploid plants such as Arabidopsis, maize and diploid progenitors of wheat have revealed no premeiotic association of chromosomes. Premeiotic and somatic association of chromosomes has only been previously observed in the anther tissues and xylem vessel cells of developing roots in polyploid plants such as hexaploid and tetraploid wheat, polyploid relatives of wheat and artificial polyploids made from the progenitor diploids of wheat. This suggested that this association was confined specifically to polyploids or was induced by polyploidy. However, we developed procedures for in situ hybridization on structurally well-preserved tissue sections of rice, and analysed two diploid rice species (Oryza sativa and O. punctata). Contrary to expectation, this has revealed that centromeres and telomeres also associate both in the xylem vessel cells of developing root and in undifferentiated anther cells in these diploids. However, in contrast to wheat and related polyploids, where the initial association in undifferentiated anthers is between either non-homologous or related chromosomes, and not homologous chromosomes, the initial association of rice chromosomes seems to be between homologues. Thus, in contrast to the diploid dicot model Arabidopsis, meiotic studies on the diploid model cereal, rice, will now need to take into account the effects of premeiotic chromosome association.
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Acknowledgements
This work was supported by grants from the Spanish Ministerio de Ciencia y Tecnología (P. Prieto), programme PRAXIS XXI, Portugal (A.P. Santos) and the UK Biotechnology and Biological Sciences Research Council.
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Communicated by E. Nigg
Pilar Prieto and Ana Paula Santos are joint first authors.
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Prieto, P., Santos, A.P., Moore, G. et al. Chromosomes associate premeiotically and in xylem vessel cells via their telomeres and centromeres in diploid rice (Oryza sativa). Chromosoma 112, 300–307 (2004). https://doi.org/10.1007/s00412-004-0274-8
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DOI: https://doi.org/10.1007/s00412-004-0274-8