Skip to main content
SpringerLink
Log in

Relative biological effectiveness of 12C and 28Si radiation in C57BL/6J mice

  • Original Paper
  • Published:
Radiation and Environmental Biophysics Aims and scope Submit manuscript

Abstract

Study of heavy ion radiation–induced effects on mice could provide insight into the human health risks of space radiation exposure. The purpose of the present study is to assess the relative biological effectiveness (RBE) of 12C and 28Si ion radiation, which has not been reported previously in the literature. Female C57BL/6J mice (n = 15) were irradiated using 4–8 Gy of 28Si (300 MeV/nucleon energy; LET 70 keV/μm) and 5–8 Gy of 12C (290 MeV/nucleon energy; LET 13 keV/μm) ions. Post-exposure, mice were monitored regularly, and their survival observed for 30 days. The LD50/30 dose (the dose at which 50 % lethality occurred by 30-day post-exposure) was calculated from the survival curve and was used to determine the RBE of 28Si and 12C in relation to γ radiation. The LD50/30 for 28Si and 12C ion is 5.17 and 7.34 Gy, respectively, and the RBE in relation to γ radiation (LD50/30—7.25 Gy) is 1.4 for 28Si and 0.99 for 12C. Determination of RBE of 28Si and 12C for survival in mice is not only important for space radiation risk estimate studies, but it also has implications for HZE radiation in cancer therapy.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3

Similar content being viewed by others

References

  • Ainsworth EJ (1986) Early and late mammalian responses to heavy charged particles. Adv Space Res 6:153–165

    Article  ADS  Google Scholar 

  • Alpen EL, Powers-Risius P (1981) The relative biological effect of high-Z, high-LET charged particles for spermatogonial killing. Radiat Res 88:132–143

    Article  Google Scholar 

  • Alpen EL, Powers-Risius P, McDonald M (1980) Survival of intestinal crypt cells after exposure to high Z, high-energy charged particles. Radiat Res 83:677–687

    Article  Google Scholar 

  • Alpen EL, Powers-Risius P, Curtis SB, DeGuzman R, Fry RJ (1994) Fluence-based relative biological effectiveness for charged particle carcinogenesis in mouse Harderian gland. Adv Space Res 14:573–581

    Article  ADS  Google Scholar 

  • Aoki M, Furusawa Y, Yamada T (2000) LET dependency of heavy-ion induced apoptosis in V79 cells. J Radiat Res (Tokyo) 41:163–175

    Article  Google Scholar 

  • Asaithamby A, Uematsu N, Chatterjee A, Story MD, Burma S, Chen DJ (2008) Repair of HZE-particle-induced DNA double-strand breaks in normal human fibroblasts. Radiat Res 169:437–446

    Article  Google Scholar 

  • Becker D, Elsasser T, Tonn T, Seifried E, Durante M, Ritter S, Fournier C (2009) Response of human hematopoietic stem and progenitor cells to energetic carbon ions. Int J Radiat Biol 85:1051–1059

    Article  Google Scholar 

  • Blakely EA, Tobias CA, Yang TC, Smith KC, Lyman JT (1979) Inactivation of human kidney cells by high-energy monoenergetic heavy-ion beams. Radiat Res 80:122–160

    Article  Google Scholar 

  • Brooks A, Bao S, Rithidech K, Couch LA, Braby LA (2001) Relative effectiveness of HZE iron-56 particles for the induction of cytogenetic damage in vivo. Radiat Res 155:353–359

    Article  Google Scholar 

  • Chen DJ, Tsuboi K, Nguyen T, Yang TC (1994) Charged-particle mutagenesis II. Mutagenic effects of high energy charged particles in normal human fibroblasts. Adv Space Res 14:347–354

    Article  ADS  Google Scholar 

  • Cucinotta FA, Townsend LW, Wilson JW, Golightly MJ, Weyland M (1994) Analysis of radiation risk from alpha particle component of solar particle events. Adv Space Res 14:661–670

    Article  ADS  Google Scholar 

  • Cucinotta FA, Schimmerling W, Wilson JW, Peterson LE, Badhwar GD, Saganti PB, Dicello JF (2001) Space radiation cancer risks and uncertainties for Mars missions. Radiat Res 156:682–688

    Article  Google Scholar 

  • Cucinotta FA, Wu H, Shavers MR, George K (2003) Radiation dosimetry and biophysical models of space radiation effects. Gravit Space Biol Bull 16:11–18

    Google Scholar 

  • Curtis SB, Townsend LW, Wilson JW, Powers-Risius P, Alpen EL, Fry RJ (1992) Fluence-related risk coefficients using the Harderian gland data as an example. Adv Space Res 12:407–416

    Article  ADS  Google Scholar 

  • Datta K, Neumann RD, Winters TA (2005) Characterization of complex apurinic/apyrimidinic-site clustering associated with an authentic site-specific radiation-induced DNA double-strand break. Proc Natl Acad Sci USA 102:10569–10574

    Article  ADS  Google Scholar 

  • Datta K, Suman S, Trani D, Doiron K, Rotolo JA, Kallakury BV, Kolesnick R, Cole MF, Fornace AJJ (2012) Accelerated hematopoietic toxicity by high energy (56)Fe radiation. Int J Radiat Biol 88:213–222

    Article  Google Scholar 

  • Hada M, Sutherland BM (2006) Spectrum of complex DNA damages depends on the incident radiation. Radiat Res 165:223–230

    Article  Google Scholar 

  • Hall EJ, Giaccia AJ (2006) Radiobiology for the radiologist, pp 1–521

  • Hamilton SA, Pecaut MJ, Gridley DS, Travis ND, Bandstra ER, Willey JS, Nelson GA, Bateman TA (2006) A murine model for bone loss from therapeutic and space-relevant sources of radiation. J Appl Physiol 101:789–793

    Article  Google Scholar 

  • Hayatsu K, Hareyama M, Kobayashi S, Yamashita NKS, Hasebe N (2009) HZE Particle and Neutron Dosages from Cosmic Rays on the Lunar Surface. J Phys Soc Jpn 78:149–152

    Article  Google Scholar 

  • Hirayama R, Furusawa Y, Fukawa T, Ando K (2005) Repair kinetics of DNA-DSB induced by X-rays or carbon ions under oxic and hypoxic conditions. J Radiat Res (Tokyo) 46:325–332

    Article  Google Scholar 

  • Kato TA, Tsuda A, Uesaka M, Fujimori A, Kamada T, Tsujii H, Okayasu R (2011) In vitro characterization of cells derived from chordoma cell line U-CH1 following treatment with X-rays, heavy ions and chemotherapeutic drugs. Radiat Oncol 6:116

    Article  Google Scholar 

  • Kawata T, Durante M, Furusawa Y, George K, Takai N, Wu H, Cucinotta FA (2001) Dose–response of initial G2-chromatid breaks induced in normal human fibroblasts by heavy ions. Int J Radiat Biol 77:165–174

    Article  Google Scholar 

  • Lett JT (1992) Damage to cellular DNA from particulate radiations, the efficacy of its processing and the radiosensitivity of mammalian cells. Emphasis on DNA double strand breaks and chromatin breaks. Radiat Environ Biophys 31:257–277

    Article  Google Scholar 

  • Monobe M, Ando K (2002) Drinking beer reduces radiation-induced chromosome aberrations in human lymphocytes. J Radiat Res (Tokyo) 43:237–245

    Article  Google Scholar 

  • Rodriguez A, Alpen EL, Powers-Risius P (1992) The RBE-LET relationship for rodent intestinal crypt cell survival, testes weight loss, and multicellular spheroid cell survival after heavy-ion irradiation. Radiat Res 132:184–192

    Article  Google Scholar 

  • Schimmerling W, Wilson JW, Cucinotta F, Kim MH (1998) Evaluation of risk from space radiation with high-energy heavy ion beams. Phys Med 14(Suppl 1):29–38

    Google Scholar 

  • Suzuki M, Tsuruoka C, Uchihori Y, Kitamura H, Liu CH (2009) Radiation-quality dependent cellular response in mutation induction in normal human cells. J Radiat Res (Tokyo) 50:395–399

    Article  Google Scholar 

  • Takiguchi Y, Miyamoto T, Nagao K, Kuriyama T (2007) Assessment of the homogeneous efficacy of carbon ions in the spread-out Bragg peak for human lung cancer cell lines. Radiat Med 25:272–277

    Article  Google Scholar 

  • Terato H, Watari H, Shimazaki Y, Hirayama R, Furusawa Y, Ide H (2008) Analysis for complexity of clustered DNA damage generated by heavy ion beams. Nucleic Acids Symp Ser (Oxf) 52:443–444

    Article  Google Scholar 

  • Tochilin G, Shumway B, Kohler G, Bond VP (1959) Re-evaluation of fast neutron RBE values on the basis of improved cross-section data. Radiat Res 11:343–344

    Article  Google Scholar 

  • Townsend LW (2005) Implications of the space radiation environment for human exploration in deep space. Radiat Prot Dosimetry 115:44–50

    Article  Google Scholar 

  • Trani D, Datta K, Doiron K, Kallakury B, Fornace AJJ (2010) Enhanced intestinal tumor multiplicity and grade in vivo after HZE exposure: mouse models for space radiation risk estimates. Radiat Environ Biophys 49:389–396

    Article  Google Scholar 

  • Tsuruoka C, Suzuki M, Kanai T, Fujitaka K (2005) LET and ion species dependence for cell killing in normal human skin fibroblasts. Radiat Res 163:494–500

    Article  Google Scholar 

  • Tsuruoka C, Suzuki M, Hande MP, Furusawa Y, Anzai K, Okayasu R (2008) The difference in LET and ion species dependence for induction of initially measured and non-rejoined chromatin breaks in normal human fibroblasts. Radiat Res 170:163–171

    Article  Google Scholar 

Download references

Acknowledgments

This work is supported by National Aeronautics and Space Administration (NASA) Grant #NNX07AH70G and NNX09AU95G. Dr. D. Trani was supported by the National Space Biomedical Research Institute (NSBRI) through NCC 9-58. We are indebted to the members of the NASA Space Radiation Laboratory at the Brookhaven National Laboratory for extending their excellent support for this study.

Author information

Authors and Affiliations

  1. Department of Biochemistry and Molecular & Cell Biology, Lombardi Comprehensive Cancer Center, Georgetown University, Room E504 Research Building, 3970 Reservoir Rd., NW, Washington, DC, 20057-1468, USA

    Shubhankar Suman, Kamal Datta, Daniela Trani, Evagelia C. Laiakis, Steven J. Strawn & Albert J. Fornace Jr.

  2. Center of Excellence in Genomic Medicine Research (CEGMR), King Abdulaziz University, Jeddah, Saudi Arabia

    Albert J. Fornace Jr.

Authors
  1. Shubhankar Suman
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Kamal Datta
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Daniela Trani
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Evagelia C. Laiakis
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Steven J. Strawn
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Albert J. Fornace Jr.
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding authors

Correspondence to Kamal Datta or Albert J. Fornace Jr..

Rights and permissions

Reprints and permissions

About this article

Cite this article

Suman, S., Datta, K., Trani, D. et al. Relative biological effectiveness of 12C and 28Si radiation in C57BL/6J mice. Radiat Environ Biophys 51, 303–309 (2012). https://doi.org/10.1007/s00411-012-0418-9

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00411-012-0418-9

Keywords

Search

Navigation