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Evaluation of the cytogenetic effects of 131I preceded by recombinant human thyrotropin (rhTSH) in peripheral lymphocytes of Wistar rats

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Abstract

The present study was carried out to investigate the cytogenetic effects of therapeutic exposure to radioiodine preceded by rhTSH in an animal model. Three groups of Wistar rats (n = 6) were used: one group was treated only with 131I (11.1 MBq/animal); the other two groups received rhTSH (1.2 μg/rat of either Thyrogen or rhTSH-IPEN, respectively) 24 h before administration of radioiodine. The percentage of lymphocytes with chromosome aberrations and the average number of aberrations and of dicentrics per cell were determined on blood samples collected 24 h, 7 and 30 days after administration of 131I. The data show that the treatment with radioiodine alone or associated with rhTSH resulted in a greater quantity of chromosome alterations in relation to basal values after 24 h, with a gradual decline after 7 and 30 days of treatment. An increase in chromosome alterations was also seen after rhTSH treatment alone. Neither of the treatments, i.e., with 131I alone or associated with hormone, resulted in an aneugenic effect or influenced the kinetics of cellular proliferation in rat blood lymphocytes. There was no significant difference between the cytogenetic effects of Thyrogen and rhTSH-IPEN treatment. These data suggest that the treatment with radioiodine, associated or not with rhTSH, affects to a limited extent a relatively small number of cells although the occurrence of late stochastic effects could not be discarded.

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Acknowledgments

The authors are grateful to Maria Neide F. Mascarenhas for her careful and excellent assistance with the animals during experiments. We thank the “Centro de Radiofarmácia” of the IPEN-CNEN/SP, Brazil, for providing Na131I for the experiments. The work was supported by FAPESP (Project 02/09872–6).

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Authors and Affiliations

  1. Centro de Biotecnologia, Instituto de Pesquisas Energéticas e Nucleares, IPEN-CNEN/SP, Cidade Universitária, Av. Prof. Lineu Prestes, 2242, Caixa Postal 11049, CEP 05508-900, São Paulo, Brazil

    Márcia Augusta da Silva, Maria Teresa Carvalho Pinto Ribela, Paolo Bartolini & Kayo Okazaki

  2. Centro de Medicina Nuclear, Faculdade de Medicina da Universidade de São Paulo, R.Ouvídio Pires de Campos, s/n, 2 andar, São Paulo, Brazil

    Maria Inês Calil Cury Guimarães & Carlos Alberto Buchpiguel

  3. Centro de Engenharia Nuclear, Instituto de Pesquisas Energéticas e Nucleares, IPEN-CNEN/SP, São Paulo, Brazil

    Hélio Yoriyaz

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  1. Márcia Augusta da Silva
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  2. Maria Inês Calil Cury Guimarães
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Correspondence to Kayo Okazaki.

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da Silva, M.A., Calil Cury Guimarães, M.I., Yoriyaz, H. et al. Evaluation of the cytogenetic effects of 131I preceded by recombinant human thyrotropin (rhTSH) in peripheral lymphocytes of Wistar rats. Radiat Environ Biophys 47, 453–461 (2008). https://doi.org/10.1007/s00411-008-0189-5

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