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Elevated Tenascin-C Levels in Bronchoalveolar Lavage Fluid of Patients with Sarcoidosis

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Abstract

Background

Sarcoidosis is a disease characterized by granulomatous lesions involving multiple organ systems. The etiology of sarcoidosis remains unknown, and reliable biomarkers have not been identified. Tenascin-C is an extracellular matrix molecule expressed during wound healing in various tissues. The present study aimed to investigate the role of tenascin-C in sarcoidosis.

Methods

Enzyme-linked immunosorbent assays were used to measure tenascin-C levels in serum and bronchoalveolar lavage fluid (BALF) from 31 patients with sarcoidosis and 15 healthy individuals. Relationships between tenascin-C concentrations in BALF and serum samples and clinical parameters in patients with sarcoidosis were evaluated.

Results

BALF tenascin-C levels were significantly higher in patients with sarcoidosis than in healthy individuals, but serum levels were no different. BALF tenascin-C levels showed positive correlations with serum lactic dehydrogenase levels and the ratio of lymphocytes in BALF. BALF tenascin-C levels were also higher in patients with parenchymal infiltration on chest radiographs than in those without.

Conclusions

The present results demonstrated that the BALF tenascin-C level was correlated with pulmonary infiltrates on chest radiographs in patients with sarcoidosis. Although measurement of serum tenascin-C levels has a limited role and measurement of BALF tenascin-C levels might be impractical, tenascin-C in the lung might play a role in the pathogenesis of sarcoidosis. Further studies are necessary to determine the role of BALF tenascin-C in sarcoidosis.

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References

  1. Miyoshi S, Hamada H, Kadowaki T, Hamaguchi N, Ito R, Irifune K, Higaki J (2010) Comparative evaluation of serum markers in pulmonary sarcoidosis. Chest 137:1391–1397

    Article  PubMed  CAS  Google Scholar 

  2. Morgenthau AS, Iannuzzi MC (2011) Recent advances in sarcoidosis. Chest 139:174–182

    Article  PubMed  Google Scholar 

  3. Baughman RP, Culver DA, Judson MA (2011) A concise review of pulmonary sarcoidosis. Am J Respir Crit Care Med 183:573–581

    Article  PubMed  CAS  Google Scholar 

  4. Baughman RP, Lower EE (2011) Who dies from sarcoidosis and why? Am J Respir Crit Care Med 183:1446–1447

    Article  PubMed  Google Scholar 

  5. Brellier F, Tucker RP, Chiquet-Ehrismann R (2009) Tenascins and their implications in diseases and tissue mechanics. Scand J Med Sci Sports 19:511–519

    Article  PubMed  CAS  Google Scholar 

  6. Tucker RP, Chiquet-Ehrismann R (2009) The regulation of tenascin expression by tissue microenvironments. Biochim Biophys Acta 1793:888–892

    Article  PubMed  CAS  Google Scholar 

  7. Kaarteenaho-Wiik R, Mertaniemi P, Sajanti E, Soini Y, Paakko P (1998) Tenascin is increased in epithelial lining fluid in fibrotic lung disorders. Lung 176:371–380

    Article  PubMed  CAS  Google Scholar 

  8. Kaarteenaho-Wiik R, Sademies O, Paakko P, Risteli J, Soini Y (2007) Extracellular matrix proteins and myofibroblasts in granulomas of sarcoidosis, atypical mycobacteriosis, and tuberculosis of the lung. Hum Pathol 38:147–153

    Article  PubMed  CAS  Google Scholar 

  9. American Thoracic Society/European Respiratory Society International Multidisciplinary Consensus Classification of the Idiopathic Interstitial Pneumonias (2002) This joint statement of the American Thoracic Society (ATS), and the European Respiratory Society (ERS) was adopted by the ATS board of directors, June 2001 and by the ERS Executive Committee, June 2001. Am J Respir Crit Care Med 165:277–304

    Google Scholar 

  10. [No authors listed] (1999) Statement on sarcoidosis. Joint Statement of the American Thoracic Society (ATS), the European Respiratory Society (ERS) and the World Association of Sarcoidosis and Other Granulomatous Disorders (WASOG) adopted by the ATS Board of Directors and by the ERS Executive Committee, February 1999. Am J Respir Crit Care Med 160:736–755

    Google Scholar 

  11. Sakamoto N, Mukae H, Fujii T, Kakugawa T, Kaida H, Kadota J, Kohno S (2004) Soluble form of Fas and Fas ligand in serum and bronchoalveolar lavage fluid of individuals infected with human T-lymphotropic virus type 1. Respir Med 98:213–219

    Article  PubMed  Google Scholar 

  12. Chiquet-Ehrismann R, Chiquet M (2003) Tenascins: regulation and putative functions during pathological stress. J Pathol 200:488–499

    Article  PubMed  CAS  Google Scholar 

  13. Hisatomi K, Sakamoto N, Mukae H, Hayashi T, Amenomori M, Ishimoto H, Fujita H, Ishii H, Nakayama S, Ishimatsu Y, Kohno S (2009) Elevated levels of tenascin-C in patients with cryptogenic organizing pneumonia. Intern Med 48:1501–1507

    Article  PubMed  Google Scholar 

  14. Lofdahl M, Kaarteenaho R, Lappi-Blanco E, Tornling G, Skold MC (2011) Tenascin-C and alpha-smooth muscle actin positive cells are increased in the large airways in patients with COPD. Respir Res 12:48

    Article  PubMed  Google Scholar 

  15. Laitinen A, Altraja A, Kampe M, Linden M, Virtanen I, Laitinen LA (1997) Tenascin is increased in airway basement membrane of asthmatics and decreased by an inhaled steroid. Am J Respir Crit Care Med 156:951–958

    PubMed  CAS  Google Scholar 

  16. Kaarteenaho-Wiik R, Tani T, Sormunen R, Soini Y, Virtanen I, Paakko P (1996) Tenascin immunoreactivity as a prognostic marker in usual interstitial pneumonia. Am J Respir Crit Care Med 154:511–518

    PubMed  CAS  Google Scholar 

  17. Kuhn C, Mason RJ (1995) Immunolocalization of SPARC, tenascin, and thrombospondin in pulmonary fibrosis. Am J Pathol 147:1759–1769

    PubMed  CAS  Google Scholar 

  18. Danila E, Jurgauskiene L, Norkuniene J, Malickaite R (2009) BAL fluid cells in newly diagnosed pulmonary sarcoidosis with different clinical activity. Ups J Med Sci 114:26–31

    Article  PubMed  Google Scholar 

  19. Clark RA, Erickson HP, Springer TA (1997) Tenascin supports lymphocyte rolling. J Cell Biol 137:755–765

    Article  PubMed  CAS  Google Scholar 

  20. Lieberman J, Schleissner LA, Nosal A, Sastre A, Mishkin FS (1983) Clinical correlations of serum angiotensin-converting enzyme (ACE) in sarcoidosis. A longitudinal study of serum ACE, 67gallium scans, chest roentgenograms, and pulmonary function. Chest 84:522–528

    Article  PubMed  CAS  Google Scholar 

  21. Prior C, Barbee RA, Evans PM, Townsend PJ, Primett ZS, Fyhrquist F, Gronhagen-Riska C, Haslam PL (1990) Lavage versus serum measurements of lysozyme, angiotensin converting enzyme and other inflammatory markers in pulmonary sarcoidosis. Eur Respir J 3:1146–1154

    PubMed  CAS  Google Scholar 

  22. Ainslie GM, Poulter LW, du Bois RM (1989) Relation between immunocytological features of bronchoalveolar lavage fluid and clinical indices in sarcoidosis. Thorax 44:501–509

    Article  PubMed  CAS  Google Scholar 

  23. Grutters JC (2003) Serum soluble interleukin-2 receptor measurement in patients with sarcoidosis: a clinical evaluation. Chest 124:186–195

    Article  PubMed  CAS  Google Scholar 

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Acknowledgments

The authors thank Mr. Atsushi Yokoyama (Nagasaki University Hospital) for excellent technical assistance. This study was supported in part by KAKENHI, Nonprofit Organization Aimed to Support Community Medicine Research in Nagasaki, and a grant to the Diffuse Lung Diseases Research Group from the Ministry of Health, Labour and Welfare, Japan.

Conflict of interest

The authors have no conflicts of interest or financial ties to disclose.

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Authors and Affiliations

  1. Second Department of Internal Medicine, Nagasaki University School of Medicine, 1-7-1 Sakamoto, Nagasaki, 852-8501, Japan

    Hanako Fujita, Noriho Sakamoto, Yuji Ishimatsu, Tomoyuki Kakugawa, Shota Nakashima, Shintaro Hara, Atsuko Hara & Shigeru Kohno

  2. Department of Respiratory Medicine, University of Occupational and Environmental Health, Kitakyushu, Fukuoka, Japan

    Hiroshi Mukae

Authors
  1. Hanako Fujita
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  2. Noriho Sakamoto
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  3. Yuji Ishimatsu
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  4. Tomoyuki Kakugawa
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  5. Shota Nakashima
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  6. Shintaro Hara
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  7. Atsuko Hara
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  8. Hiroshi Mukae
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  9. Shigeru Kohno
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Correspondence to Noriho Sakamoto.

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Fujita, H., Sakamoto, N., Ishimatsu, Y. et al. Elevated Tenascin-C Levels in Bronchoalveolar Lavage Fluid of Patients with Sarcoidosis. Lung 190, 537–543 (2012). https://doi.org/10.1007/s00408-012-9400-1

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  • DOI: https://doi.org/10.1007/s00408-012-9400-1

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