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Staphylococcal enterotoxin B induced expression of IL-17A in nasal epithelial cells and its association with pathogenesis of nasal polyposis

  • Rhinology
  • Published:
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Abstract

Interleukin (IL)-17A is a highly inflammatory cytokine and is known to be produced by Th17 cells. The importance of IL-17A expression in nasal epithelial cells is not well understood. The goal of this study is to explore the expression of IL-17A in nasal epithelial cells in vivo and in vitro. IL-17A and staphylococcal enterotoxin B (SEB) were detected by immunofluorescence (IF) in nasal epithelial cells of control mucosa (n = 10) and nasal polyps (n = 20). Expression of IL-17A, RORC, IL-6, and TGF-β1 was also measured by RT-PCR in the tissue of control nasal mucosa (n = 10) and nasal polyps (n = 20). IL-17A expression was evaluated in the human nasal epithelial cells after SEB stimulation. Finally, IL-17A expression was demonstrated by immunohistochemistry and IF following intranasal SEB instillation in mice. Expression of IL-17A in nasal epithelial cells was higher in nasal polyps compared to control mucosa. There was a significant correlation between IL-17A and SEB detection in nasal polyps using IF. SEB increased IL-17A expression in human nasal epithelial cells, and in epithelial cells of SEB instilled mice. In conclusion, SEB exposure of nasal epithelial cells induces the enhanced expression of IL-17A. SEB may be involved in pathogenesis of nasal polyps by enhancing IL-17A expression in epithelial cells in nasal polyps.

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Acknowledgments

We thank the study participants, all joint research workers, and Dong-Woon Kim from Department of Anatomy for assisting with the double immunofluorescence study. We also thank the patients who provided samples for the study.

This work (research) was supported by Chungnam National University Hospital Research Fund 2010. This research was supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology (2011-0009790).

Conflict of interest

No potential conflict of interest relevant to this article was reported.

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Authors and Affiliations

  1. Department of Otorhinolaryngology-Head and Neck Surgery, Chungnam National University School of Medicine, 282 Munhwa-ro, Jung-gu, Daejeon, 301-721, Korea

    Jun Jin, Young Hoon Yoon, Seo Young Kwak, Ki-Sang Rha & Yong Min Kim

  2. Research Institute for Medical Science, Chungnam National University School of Medicine, Daejeon, Korea

    Ki-Sang Rha & Yong Min Kim

  3. Yanbian University Hospital, Yanji, Jilin, China

    Jun Jin

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  1. Jun Jin
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  2. Young Hoon Yoon
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  3. Seo Young Kwak
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  4. Ki-Sang Rha
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  5. Yong Min Kim
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Correspondence to Yong Min Kim.

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Jin, J., Yoon, Y.H., Kwak, S.Y. et al. Staphylococcal enterotoxin B induced expression of IL-17A in nasal epithelial cells and its association with pathogenesis of nasal polyposis. Eur Arch Otorhinolaryngol 271, 525–534 (2014). https://doi.org/10.1007/s00405-013-2589-7

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  • DOI: https://doi.org/10.1007/s00405-013-2589-7

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