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DNA copy gains of tumor-related genes in vestibular schwannoma

  • Otology
  • Published:
European Archives of Oto-Rhino-Laryngology Aims and scope Submit manuscript

Abstract

DNA copy gains are a common event in tumor growth. This study determines the gene dosage/amplification of seven tumor-related genes in patients undergoing vestibular schwannoma (VS) surgery and analyzes its clinical implications. Thirty-three patients undergoing surgery for VS were studied. Seven genes (EGFR, ERBB2, ERBB3, ERBB4, MDM2, MDM4, and NMYC) were analyzed by Quantitative real-time PCR. Copy gains were correlated with demographic, clinical and radiological data. Of the 33 samples, 48 % were positive for copy gains in at least one gene. There were no positive samples for gene amplification. A clinical correlation between tumor size and copy gains of ERBB2 was found. Patients with copy gains of this gene had larger tumors measured by diameter (p = 0.027) and volume (p = 0.005). Copy gains of EGFR, ERBB2, ERBB4, and MDM4 were associated with preoperative tinnitus. Contrary to other tumors of the central nervous system, development of VS does not appear to involve gene amplification. However, copy gains of certain tumor-related genes may play a role in the biological behavior of these neoplasms. Our findings support the role of ERBB2 in VS development and growth

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Acknowledgments

Support for this work was provided by grants 07/0577 and 10/1972 from FIS, Ministerio de Sanidad.

Conflict of interest

The authors have no conflict of interest.

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Authors and Affiliations

  1. Department of Otolaryngology, Hospital La Paz/Autónoma University School of Medicine, IdiPAZ, Pº de la Castellana, 261, 28046, Madrid, Spain

    Luis Lassaletta & Javier Gavilán

  2. Unidad de Investigación, Laboratorio Oncogenetica Molecular, Hospital La Paz/Autónoma University School of Medicine, IdiPAZ, Madrid, Spain

    Miguel Torres-Martín & Juan Antonio Rey

  3. Preventive Medicine and Public Health Teaching and Research Unit, Department of Health Sciences, Universidad Rey Juan Carlos, Madrid, Spain

    Jesús San-Román-Montero

  4. Brain Tumor Biology Unit-CIFA, University of Navarra School of Sciences, Pamplona, Spain

    Javier S. Castresana

Authors
  1. Luis Lassaletta
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  2. Miguel Torres-Martín
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  4. Javier S. Castresana
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Correspondence to Luis Lassaletta.

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Lassaletta, L., Torres-Martín, M., San-Román-Montero, J. et al. DNA copy gains of tumor-related genes in vestibular schwannoma. Eur Arch Otorhinolaryngol 270, 2433–2438 (2013). https://doi.org/10.1007/s00405-012-2269-z

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  • DOI: https://doi.org/10.1007/s00405-012-2269-z

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