Skip to main content

Advertisement

SpringerLink
Log in

Prevalence and clinical features of human papillomavirus in head and neck squamous cell carcinoma in Okinawa, southern Japan

  • Head & Neck
  • Published:
European Archives of Oto-Rhino-Laryngology Aims and scope Submit manuscript

Abstract

Previous studies from Okinawa, a subtropical island in southern Japan, demonstrated a higher prevalence of human papillomavirus (HPV) in oral carcinoma and a higher incidence of oral and pharyngeal carcinoma than those for mainland Japan. The present study aims to investigate epidemiologic and clinical features of HPV in head and neck squamous cell carcinoma (HNSCC) in Okinawa. A total of 150 DNA samples from 150 Okinawan patients with head and neck squamous cell carcinoma (HNSCC) were screened for HPV sequences by PCR using three consensus primer sets, and HPV types were determined by direct sequencing. The samples were consisted of 46 cases from the hypopharynx, 44 from the oropharynx, 16 from the larynx, 25 from the oral cavity, 10 from the maxillary sinus, and 9 from the nasopharynx. HPV DNA was detected in 45 (30.0%) HNSCCs, and HPV-16 was identified in 86.7% of positive specimens. The highest prevalence of the HPV sequence was found in oropharyngeal carcinomas (50.0%), especially in tonsillar cancer (63.6%). Multivariate analysis showed that oropharyngeal carcinoma (P = 0.002; OR = 5.34; 95% CI = 1.83–15.58), oral cavity carcinoma (P = 0.012; OR = 4.94; 95% CI = 1.43–17.10), and histological poor differentiation (P = 0.011; OR = 4.25; 95% CI = 1.39–13.04) each independently increased the prevalence of HPV infection. The present study reveals that patients with HNSCC, e.g., oropharyngeal and oral cavity carcinomas, in Okinawa have relatively high HPV-16 positive rates and low HPV-18 positive rates comparing with mainland Japan.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Parkin DM, Bray F, Ferlay J et al (2005) Global cancer statistics, 2002. CA Cancer J Clin 55:74–108

    Article  PubMed  Google Scholar 

  2. Hashibe M, Brennan P, Chuang SC et al (2009) Interaction between tobacco and alcohol use and the risk of head and neck cancer: pooled analysis in the International Head and Neck Cancer Epidemiology Consortium. Cancer Epidemiol Biomarkers Prev 18:541–550

    Article  PubMed  CAS  Google Scholar 

  3. Gillison ML, Koch WM, Capone RB et al (2000) Evidence for a causal association between human papillomavirus and a subset of head and neck cancers. J Natl Cancer Inst 92:709–720

    Article  PubMed  CAS  Google Scholar 

  4. Klussmann JP, Weissenborn SJ, Wieland U et al (2001) Prevalence, distribution, and viral load of human papillomavirus 16 DNA in tonsillar carcinomas. Cancer 92:2875–2884

    Article  PubMed  CAS  Google Scholar 

  5. D’Souza G, Kreimer AR, Viscidi R et al (2007) Case-control study of human papillomavirus and oropharyngeal cancer. N Engl J Med 356:1944–1956

    Article  PubMed  Google Scholar 

  6. Schlecht NF, Burk RD, Adrien L et al (2007) Gene expression profiles in HPV-infected head and neck cancer. J Pathol 213:283–293

    Article  PubMed  CAS  Google Scholar 

  7. Ernster JA, Sciotto CG, O’Brien MM et al (2007) Rising incidence of oropharyngeal cancer and the role of oncogenic human papilloma virus. Laryngoscope 117:2115–2128

    Article  PubMed  Google Scholar 

  8. Li W, Tran N, Lee SC et al (2007) New evidence for geographic variation in the role of human papillomavirus in tonsillar carcinogenesis. Pathology 39:217–222

    Article  PubMed  CAS  Google Scholar 

  9. Tsuhako K, Nakazato I, Miyagi J et al (2000) Comparative study of oral squamous cell carcinoma in Okinawa, Southern Japan and Sapporo in Hokkaido, Northern Japan; with special reference to human papillomavirus and Epstein-Barr virus infection. J Oral Pathol Med 29:70–79

    Article  PubMed  CAS  Google Scholar 

  10. Matsuda T, Marugame T, Kamo K, Japan Cancer Surveillance Research Group et al (2009) Cancer incidence and incidence rates in Japan in 2003: based on data from 13 population-based cancer registries in the Monitoring of Cancer Incidence in Japan (MCIJ) Project. Jpn J Clin Oncol 39:850–858

    Article  PubMed  Google Scholar 

  11. Barnes L, Eveson JW, Reichart P, Sidransky D (eds) (2005) World Health Organization classification of tumours. Pathology and genetics of head and neck tumours. IARC Press, Lyon

    Google Scholar 

  12. Saiki RK, Scharf S, Faloona F et al (1985) Enzymatic amplification of beta-globin genomic sequences and restriction site analysis for diagnosis of sickle cell anemia. Science 230:1350–1354

    Article  PubMed  CAS  Google Scholar 

  13. de Roda Husman AM, Walboomers JM, van den Brule AJ et al (1995) The use of general primers GP5 and GP6 elongated at their 3’ ends with adjacent highly conserved sequences improves human papillomavirus detection by PCR. J Gen Virol 76:1057–1062

    Article  PubMed  Google Scholar 

  14. Manos MM, Ting Y, Wright DK et al (1989) Use of polymerase chain reaction amplification for the detection of genital human papillomaviruses. Cancer cells 7:209–214

    CAS  Google Scholar 

  15. Smits HL, Tieben LM, Tjong-A-Hung SP et al (1992) Detection and typing of human papillomaviruses present in fixed and stained archival cervical smears by a consensus polymerase chain reaction and direct sequence analysis allow the identification of a broad spectrum of human papillomavirus types. J Gen Virol 73(Pt12):3263–3268

    Article  PubMed  CAS  Google Scholar 

  16. Mork J, Lie AK, Glattre E et al (2001) Human papillomavirus infection as a risk factor for squamous-cell carcinoma of the head and neck. N Engl J Med 344:1125–1131

    Article  PubMed  CAS  Google Scholar 

  17. Hoffmann M, Ihloff AS, Gorogh T et al (2005) Human papillomaviruses in head and neck cancer: 8 year-survival-analysis of 73 patients. Cancer Lett 218:199–206

    Article  PubMed  CAS  Google Scholar 

  18. Chen R, Sehr P, Waterboer T et al (2005) Presence of DNA of human papillomavirus 16 but no other types in tumor-free tonsillar tissue. J Clin Microbiol 43:1408–1410

    Article  PubMed  CAS  Google Scholar 

  19. Sisk J, Schweinfurth JM, Wang XT et al (2006) Presence of human papillomavirus DNA in tonsillectomy specimens. Laryngoscope 116:1372–1374

    Article  PubMed  CAS  Google Scholar 

  20. Mineta H, Ogino T, Amano HM et al (1998) Human papilloma virus (HPV) type 16 and 18 detected in head and neck squamous cell carcinoma. Anticancer Res 18:4765–4768

    PubMed  CAS  Google Scholar 

  21. Suzuki M, Inui T, Matayoshi S et al (2009) Clinical features of multiple primary cancers in patients with head and neck squamous cell carcinoma in the Ryukyu Islands. Jpn J Head Neck Cancer 35:406–411

    Google Scholar 

Download references

Acknowledgments

This study was supported by KAKENHI 22791614 (Grant-in-Aid for Young Scientists (B) from the Japan Society for the Promotion of Science) and awarded to Dr. Hasegawa, and by a grant from the Ryukyu Asia–Pacific Medical Exchange Association given to Dr. Suzuki.

Conflict of interest

The authors declare that they have no conflict of interest.

Author information

Authors and Affiliations

  1. Department of Otorhinolaryngology, Head and Neck Surgery, Graduate school of Medicine, University of the Ryukyus, 207 Uehara, Nishihara-cho, Okinawa, 903-0215, Japan

    Zeyi Deng, Masahiro Hasegawa, Sen Matayoshi, Asanori Kiyuna, Yukashi Yamashita, Hiroyuki Maeda & Mikio Suzuki

Authors
  1. Zeyi Deng
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Masahiro Hasegawa
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Sen Matayoshi
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Asanori Kiyuna
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Yukashi Yamashita
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Hiroyuki Maeda
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Mikio Suzuki
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Zeyi Deng.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Deng, Z., Hasegawa, M., Matayoshi, S. et al. Prevalence and clinical features of human papillomavirus in head and neck squamous cell carcinoma in Okinawa, southern Japan. Eur Arch Otorhinolaryngol 268, 1625–1631 (2011). https://doi.org/10.1007/s00405-011-1515-0

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00405-011-1515-0

Keywords

Search

Navigation