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The role of interleukin-16 in eosinophilic chronic rhinosinusitis

  • Rhinology
  • Published:
European Archives of Oto-Rhino-Laryngology Aims and scope Submit manuscript

Abstract

Eosinophilic granulocytes (Eos) are found in great numbers both in the tissue and in the mucus of patients suffering from chronic rhinosinusitis with polyposis (ECRS). Interleukin-16 (IL-16) is known as a highly potent chemotactic and chemoattractant molecule (ED 10−11) for Eos. In an open, explorative, controlled study we examined the presence of IL-16 in mucosa tissue, mucus and serum in patients suffering from ECRS and its association to Eos activation. Tissue and nasal mucus specimen from 10 previously untreated, non allergic ECRS-patients undergoing paranasal sinus surgery and from 10 healthy non sinusitis subjects, undergoing nasal surgery because of anatomic nasal obstruction were investigated by real-time (RT-) PCR targeting human IL-16 mRNA. Haematoxylin-eosin (HE) staining and immunohistochemistry of formalin embedded tissue and mucus were applied for detection and determination of the proportion of activated Eos (aEos) and IL-16. Serum IL-16 was analyzed by enzyme-linked-immunosorbent assay (ELISA). IL-16 mRNA and IL-16 protein levels were elevated in nasal mucus, polyp tissue and in the serum of ECRS patients compared to healthy controls. There was a high proportion of aEos in ECRS patients compared to healthy subjects. Serum IL-16, IL-16 mRNA expression and IL-16 protein in mucus and tissue specimens were significantly associated with the presence of aEos in polyps of ECRS patients. Immunohistochemically IL-16 protein was mainly expressed in aEos, mast cells, lymphocytes and epithelial cells. In conclusion our data indicate that IL-16 may stimulate the migration and persistence of activated Eos in ECRS. IL-16 production in ECRS patients is not mediated by Immunglobuline-E (IgE).

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Acknowledgments

EG2 Ab kindly provided by Karin Walcher from Pharmacia (Pfizer Austria).

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Authors and Affiliations

  1. Department of Otorhinolaryngology, Head and Neck Surgery, Medical University Graz, Auenbruggerplatz 26-28, 8036, Graz, Austria

    Andreas Lackner, Heinz Stammberger, Hannes Braun, Sabine Reinisch & Kurt Freudenschuss

  2. Institute of Hygiene, Medical University Graz, Graz, Austria

    Reinhard Bernd Raggam, Barbara Kleinhappl, Walter Buzina, Clemens Kittinger, Sonja Barth & Egon Marth

  3. Institute for Pathology, Medical University Graz, Graz, Austria

    Alfred Beham

  4. Institute for Medical Informatics, Statistics and Documentation, Medical University Graz, Graz, Austria

    Andrea Berghold

Authors
  1. Andreas Lackner
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  2. Reinhard Bernd Raggam
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  3. Heinz Stammberger
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  4. Alfred Beham
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  5. Hannes Braun
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  6. Barbara Kleinhappl
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  7. Walter Buzina
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  8. Clemens Kittinger
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  9. Sabine Reinisch
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  10. Andrea Berghold
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  11. Kurt Freudenschuss
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  12. Sonja Barth
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  13. Egon Marth
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Correspondence to Andreas Lackner.

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Andreas Lackner and Reinhard Raggam contributed equally to this article.

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Lackner, A., Raggam, R.B., Stammberger, H. et al. The role of interleukin-16 in eosinophilic chronic rhinosinusitis. Eur Arch Otorhinolaryngol 264, 887–893 (2007). https://doi.org/10.1007/s00405-007-0300-6

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  • DOI: https://doi.org/10.1007/s00405-007-0300-6

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