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The effect of folate on ischemia/reperfusion injury in a rat adnexal torsion model

  • General Gynecology
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A Correspondence to this article was published on 24 January 2022

Abstract

Purpose

The ischemia/reperfusion (I/R) injury of ovaries in adnexal torsion may have inadvertent consequences. Many agents have been studied in terms of their ability to prevent reperfusion damage to ovaries in suspected cases. In this study, folic acid, known to have antioxidative properties, was investigated to determine whether it played a role in the prevention of I/R damage in a rat ovarian torsion model.

Methods

In this experimental study, 40 female adult Wistar-Albino rats were randomly divided into five groups as control, ischemia, I/R, Fol2 (2 mg/kg folic acid), and Fol4 (4 mg/kg folic acid). In the Fol2 and Fol4 groups, folic acid was intraperitonelly administered 30 min before reperfusion. Blood samples were obtained from the tails of each rat at the second hour of reperfusion.

Results

The total oxidant status (TOS), total antioxidant status, cystatin C and folic acid levels of the five groups were investigated. Folic acid in 2 mg/kg dose could moderately increase the serum folic acid concentration (15.75–19.95 ng/ml, p < 0.05), reduce the level of cystatin C (0.18–0.12 μg/L, p < 0.05), and had a tendency to improve the oxidative stress injury (OSI: 76.05–33.06, p > 0.05), although there was no statistical difference in TOS levels (p = 0.07). Folic acid in 4 mg/kg dose, could significantly increase the serum folic acid concentration (15.75–37.65 ng/ml). However, it did not significantly reduce the level of cystatin C (0.18–0.19 μg/L, p > 0.05), and did not improve oxidative stress injury (76.05–130.58, p > 0.05).

Conclusion

Folic acid in 2 mg/kg dose might improve the ovarian I/R injury though this was not statistically significant. Further studies are required to reach a definitive conclusion about the protective effect of folic acid in I/R injury.

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Acknowledgements

All authors have accepted responsibility for the entire content of this manuscript and approved submission.

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This report has no financial support.

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Authors and Affiliations

  1. Department of Biochemistry, Ufuk University Faculty of Medicine, Mevlana Street No: 86 Balgat, 06520, Ankara, Turkey

    Tuba Çandar

  2. Department of Obstetrics and Gynecology, Ankara City Hospital, Ankara, Turkey

    Özlem Uzunlar

  3. Department of Obstetrics and Gynecology, Ufuk University Faculty of Medicine, Ankara, Turkey

    Mine Kiseli

  4. Department of Obstetrics and Gynecology, Acıbadem Ankara Hospital, Ankara, Turkey

    Sarp Özcan

Authors
  1. Tuba Çandar
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  2. Özlem Uzunlar
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  3. Mine Kiseli
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  4. Sarp Özcan
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Contributions

TC: Project development, manuscript writing, interpretation of data, OU: Project development, data analysis, MK: Manuscript writing, editing, SO: Data collection.

Corresponding author

Correspondence to Tuba Çandar.

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The authors declare that they have no conflict of interest.

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This study was approved by the Animal Research Laboratory Ethical Committee of Ankara Education and Research Hospital (04.06.2013, No: 0014).

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Çandar, T., Uzunlar, Ö., Kiseli, M. et al. The effect of folate on ischemia/reperfusion injury in a rat adnexal torsion model. Arch Gynecol Obstet 303, 1495–1500 (2021). https://doi.org/10.1007/s00404-020-05934-3

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  • DOI: https://doi.org/10.1007/s00404-020-05934-3

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