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Biological and biomechanical analysis of two types of mesenchymal stem cells for intervention in chemotherapy-induced ovarian dysfunction

  • Gynecologic Endocrinology and Reproductive Medicine
  • Published:
Archives of Gynecology and Obstetrics Aims and scope Submit manuscript

Abstract

Purpose

The study aim was to investigate the biological and biomechanical features of a chemotherapy-induced ovarian dysfunction (CIOD) rat model after intervention with human umbilical cord mesenchymal stem cells (UC-MSCs) and human amniotic mesenchymal stem cells (h-AMSCs), thus providing a biological and biomechanical research basis for clinical treatment.

Methods

The serum levels of estradiol (E2), follicle-stimulating hormone (FSH), luteinizing hormone (LH), and vascular endothelial growth factor (VEGF) in the rat CIOD models were evaluated on the 14th, 30th, 60th, and 90th day of intervention with h-AMSCs and UC-MSCs. In addition, the ovaries in each group were sampled on the 14th and 90th day of intervention for tissue morphology and tensile testing.

Results

The serum levels of E2, LH, and VEGF in the h-AMSC and UC-MSC groups were greater than in the model group, but the serum FSH level was less than in the model group, and the differences were significant (P < 0.05); the maximum tensile stress and maximum strain in h-AMSC and UC-MSC groups were significantly greater than in the model group (P < 0.05).

Conclusions

UC-MSC and h-AMSC intervention restored damaged ovarian morphology, elasticity, and toughness to a certain extent, and ovarian function showed some recovery.

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Authors and Affiliations

  1. Reproductive Center, The First Hospital of Jilin University, Changchun, 130021, China

    Yuan Pan, Liqun Zhang, Xinyue Zhang, Cong Hu & Ruizhi Liu

Authors
  1. Yuan Pan
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  2. Liqun Zhang
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  3. Xinyue Zhang
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  4. Cong Hu
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  5. Ruizhi Liu
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Corresponding author

Correspondence to Ruizhi Liu.

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Ethical approval

This study was carried out in strict accordance with the recommendations in the Guide for the Care and Use of Laboratory Animals of the National Institutes of Health. The animal use protocol has been reviewed and approved by the Institutional Animal Care and Use Committee (IACUC) of Jilin University.

Conflict of interest

All authors have no conflict of interest regarding this paper.

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Pan, Y., Zhang, L., Zhang, X. et al. Biological and biomechanical analysis of two types of mesenchymal stem cells for intervention in chemotherapy-induced ovarian dysfunction. Arch Gynecol Obstet 295, 247–252 (2017). https://doi.org/10.1007/s00404-016-4224-5

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  • DOI: https://doi.org/10.1007/s00404-016-4224-5

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