Abstract
Purpose
This preliminary study aimed at investigating the feasibility and effective of multi-scale hyperspectral imaging in detecting cervical neoplasia at both tissue and cellular levels.
Methods
In this paper, we describe a noninvasive diagnosis method with a hyperspectral imager for detection and location of cervical intraepithelial neoplasia (CIN) at multiple scales. At the macroscopic level, the hyperspectral imager was applied to capture the reflectance images of the entire cervix in vivo at a series of wavelengths. At the microscopic level, the hyperspectral imager was coupled with a microscope to collect the transmittance images of the pathological slide. The collected image data were calibrated. A wide-gap second derivative analysis was applied to differentiate CIN from other types of tissue.
Results
At both macroscopic and microscopic levels, hyperspectral imaging analysis results were consistent with those of histopathological analysis, indicating the technical feasibility of multi-scale hyperspectral imaging for cervical neoplasia detection with accuracy and efficacy.
Conclusion
We propose a multi-scale hyperspectral imaging method for noninvasive detection of cervical neoplasia. Comparison of the imaging results with those of gold standard histologic measurements demonstrates that the hyperspectral diagnostic imaging system can distinguish CIN at both tissue and cellular levels.
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Acknowledgments
This project was partially supported by Natural Science Foundation of China (Nos. 81271527 and 81327803) and the Fundamental Research Funds for the Central Universities (WK2090090013). The authors are grateful for the help of the traceable reflectance standard provided by Dr. David Allen at National Institute of Standards and Technology (NIST).
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Wang, C., Zheng, W., Bu, Y. et al. Multi-scale hyperspectral imaging of cervical neoplasia. Arch Gynecol Obstet 293, 1309–1317 (2016). https://doi.org/10.1007/s00404-015-3906-8
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DOI: https://doi.org/10.1007/s00404-015-3906-8