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Dysregulated microRNAs involved in the progression of cervical neoplasm

  • Gynecologic Oncology
  • Published:
Archives of Gynecology and Obstetrics Aims and scope Submit manuscript

Abstract

Purpose

MicroRNAs (miRNAs) exhibit dysregulated expression in human cancer and play an important role in carcinogenesis. The aim of this study was to identify a distinct miRNA expression signature for cervical cancer and cervical intraepithelial neoplasia (CIN) and to investigate the function of deregulated miRNAs in cervical carcinoma.

Methods

A miRNA microarray was used to compare miRNA expression profiles in cervical cancer, CIN and normal cervical tissues. Real-time RT-PCR was used to validate the expression of 9 miRNAs in 103 cervical tissues. Bioinformatics programs were used to predict potential target genes and their function. Functional studies were performed to characterize the effect on cervical cancer cells by overexpression of miR-218 and miR-21.

Results

We identified deregulated miRNAs in cervical cancer and high-grade squamous intraepithelial lesions (HSIL). MiR-218 was the most downregulated (0.175-fold decrease) miRNA, and miR-21 was the most upregulated (5.67-fold increase) miRNA. In addition, the expression patterns of 9 miRNAs were validated by real-time RT-PCR. Bioinformatics analyses and functional studies indicated that miR-218 and miR-21 may be involved in cancer invasion and metastasis.

Conclusion

Our study demonstrated that miRNAs are aberrantly expressed in cervical cancer and cervical preneoplastic lesions. These miRNAs may be involved in the progression of cervical neoplasm as potential tumor suppressor genes or oncogenes.

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Acknowledgments

This work was supported by the National Natural Science Foundation of China (No. 81060219) and Natural Science Fundation of Guangxi (No. 2014GXNSFAA118266)

Conflict of interest

We declare that we have no conflict of interest.

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Author notes

    Authors and Affiliations

    1. Department of Gynecology, The People’s Hospital of Guangxi Zhuang Autonomous Region, 6 Taoyuan Road, Nanning, 530021, Guanxi, People’s Republic of China

      Kangkang Zeng, Meiyi Li & Xiaoxia Hu

    2. Department of Gynecology, Renmin Hospital of Wuhan University, 99 Ziyang Road, Wuchang District, Wuhan, 430060, Hubei, People’s Republic of China

      Wenjing Zheng, Zhen Liu & Wei Zhang

    3. Department of Pathology, The People’s Hospital of Guangxi Zhuang Autonomous Region, Nanning, 530021, Guanxi, People’s Republic of China

      Xianglan Mo

    4. Research Center of Medical Science, The People’s Hospital of Guangxi Zhuang Autonomous Region, Nanning, 530021, Guanxi, People’s Republic of China

      Fei Liu

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    1. Kangkang Zeng
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    Correspondence to Wei Zhang or Xiaoxia Hu.

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    Kangkang Zeng and Wenjing Zheng have contributed equally to this work.

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    Zeng, K., Zheng, W., Mo, X. et al. Dysregulated microRNAs involved in the progression of cervical neoplasm. Arch Gynecol Obstet 292, 905–913 (2015). https://doi.org/10.1007/s00404-015-3702-5

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    • DOI: https://doi.org/10.1007/s00404-015-3702-5

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