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Effects of peritoneal fluid from endometriosis patients on the release of monocyte-specific chemokines by leukocytes

  • General Gynecology
  • Published:
Archives of Gynecology and Obstetrics Aims and scope Submit manuscript

Abstract

Purpose

Chemokines have been implicated in the pathological process of endometriosis. We compared the effects of peritoneal fluid obtained from patients with endometriosis (ePF) and controls without endometriosis (cPF) on the release of monocyte-specific CC chemokines such as monocyte chemotactic protein-1 (MCP-1), regulated upon activation normal T cell expressed and secreted (RANTES), and macrophage inflammatory protein-1α (MIP-1α) by neutrophils, monocytes, and T cells. Moreover, we evaluated the correlation between the levels of chemokines in ePF and their release by these cells.

Methods

Cells were obtained from healthy young volunteers and cultured with ePF (n = 12) or cPF (n = 8). The chemokine levels in the ePF and the supernatants of cultured cells with ePF were then measured by ELISA.

Results

There was a positive correlation between the levels of MCP-1 and MIP-1α in ePF. The addition of ePF to the cell cultures failed to increase the release of MCP-1, RANTES, and MIP-1α when compared to cPF, but the levels of RANTES in ePF were positively correlated with the release of RANTES by ePF-treated monocytes and T cells. Moreover, there was a positive correlation between the levels of RANTES and MIP-1α released by neutrophils and between the levels of MCP-1 and MIP-1α released by T cells. Finally, the levels of RANTES released by monocyte-derived macrophages and monocytes cultured with ePF were positively correlated.

Conclusions

These findings suggest that monocytes, neutrophils, and T cells release differential levels of MCP-1, RANTES, and MIP-1α in response to stimulation with ePF.

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Acknowledgments

This study was supported by the Medical Research Institute Grant (2006-29), Pusan National University Hospital, a grant of the Korea Healthcare technology R&D project, Ministry of Health &Welfare, Republic of Korea (A080416), and the National Research Foundation of Korea (NRF) grant funded by the Korea Ministry Education, Science and Technology (MEST) (R13-2002-044-04001-0).

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Authors and Affiliations

  1. Department of Obstetrics and Gynecology, Medical Research Institute, Pusan National University, Busan, 602-739, Korea

    Yong-Jin Na, Dong-Hyung Lee, Seung-Chul Kim, Jong-Kil Joo & Kyu-Sup Lee

  2. Medical Research Center for Cancer Molecular Therapy and Department of Biochemistry, School of Medicine, Dong-A University, Busan, 602-714, Korea

    Ji-Won Wang, Jun-O Jin & Jong-Young Kwak

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  1. Yong-Jin Na
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  2. Dong-Hyung Lee
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  4. Jong-Kil Joo
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  5. Ji-Won Wang
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  6. Jun-O Jin
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  7. Jong-Young Kwak
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  8. Kyu-Sup Lee
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Correspondence to Jong-Young Kwak or Kyu-Sup Lee.

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Na, YJ., Lee, DH., Kim, SC. et al. Effects of peritoneal fluid from endometriosis patients on the release of monocyte-specific chemokines by leukocytes. Arch Gynecol Obstet 283, 1333–1341 (2011). https://doi.org/10.1007/s00404-010-1583-1

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  • DOI: https://doi.org/10.1007/s00404-010-1583-1

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