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The study on role of peripheral blood mononuclear cell in HBV intrauterine infection

  • General Gynecology
  • Published:
Archives of Gynecology and Obstetrics Aims and scope Submit manuscript

Abstract

Objective

To explore the role of mother’s peripheral blood mononuclear cell (PBMC) infected with hepatitis B virus (HBV) in intrauterine transmission.

Methods

We have selected 60 cases of pregnant women with negative serum HBV DNA and positive PBMC HBV DNA from hospitalized patients. These women and their neonates acted as the experimental group. Twenty cases of pregnant women with HBV serum marker negative were selected. These women and their neonates served as the control group. Immunohistochemistry was employed to detect the expressions of HBsAg and HBcAg in cells of every placental layer and CD68 cells of placenta of the pregnant women whose neonates’ PBMC HBV DNA was positive and/or whose neonates’ serum HBV DNA positive.

Results

In the experimental group, neonatal serum HBV DNA of only four cases were positive, only eight cases’ neonatal PBMC HBV DNA were positive and four cases had HBV DNA positive in both neonatal serum and PBMC. The expressions of HBsAg and HBcAg were detected in CD68 cells of villous stroma and blood capillary in only eight cases of neonatal placenta with positive PBMC HBV DNA. HBV infection was found in cells of every layer in placenta in two of four cases with neonatal serum HBV DNA positive. The expressions of HBsAg and HBcAg were detected in trophoblastic cells, CD68 cells of villous stroma and blood capillary in two of four cases with HBV DNA positive in both neonatal serum and PBMC. In control group, no positive signals were detected in neonates and placenta.

Conclusion

HBV-infected PBMC in pregnant women may lead to intrauterine infection.

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Acknowledgments

This test was reported by state of Shaanxi Province. We obtained financial aid from The National Natural Science Fund for the study (No. 30872220). The ethical approval was obtained from Shaanxi Province Ethics Committee for the study in pregnancy.

Conflict of interest statement

None.

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Authors and Affiliations

  1. Department of Gynaecology and Obstetrics, First Hospital of Xi’an Jiaotong University, NO 277, Yanta West Road, 710061, Xi’an, Shaanxi, China

    G. Q. Bai, S. H. Li & Y. F. Yue

  2. Department of Infectious Disease, First Hospital of Xi’an Jiaotong University, NO 277, Yanta West Road, 710061, Xi’an, Shaanxi, China

    L. Shi

Authors
  1. G. Q. Bai
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  2. S. H. Li
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  3. Y. F. Yue
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  4. L. Shi
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Correspondence to G. Q. Bai.

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Bai, G.Q., Li, S.H., Yue, Y.F. et al. The study on role of peripheral blood mononuclear cell in HBV intrauterine infection. Arch Gynecol Obstet 283, 317–321 (2011). https://doi.org/10.1007/s00404-010-1366-8

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  • DOI: https://doi.org/10.1007/s00404-010-1366-8

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