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Local injury to the endometrium on the day of oocyte retrieval has a negative impact on implantation in assisted reproductive cycles: a randomized controlled trial

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Abstract

Purpose

To evaluate the effect of local injury to the endometrium on the day of oocyte retrieval on implantation and pregnancy rates in assisted reproductive cycles.

Methods

In a prospective controlled trial, a total of 156 patients, <38 years old, in their first in vitro fertilization (IVF) cycle were randomized. In 77 patients, two small endometrial samples from anterior and posterior walls of uterus were obtained with a Novak curette on the day of oocyte retrieval and in 79 patients no intervention was performed.

Results

The experimental and control patients were matched regarding women’s age, body mass index, basal FSH, duration and etiology of infertility, treatment protocol, number of retrieved oocyte, endometrial thickness, percentage of intracytoplasmic sperm injection performance, fertilization rate, the percentage of patients with good and top quality embryos, and the number of embryos transferred. The implantation rate (7.9 vs. 22.9%), clinical (12.3 vs. 32.9%; odds ratio = 0.25; 95% confidence interval = 0.12–0.66; p < 0.05) and ongoing pregnancy (9.6 vs. 29.1%; odds ratio = 0.25; 95% confidence interval = 0.10–0.64; p < 0.05) rates were significantly lower in experimental group, compared with 79 controls.

Conclusion

According to the results of this study, local injury to the endometrium on the day of oocyte retrieval disrupts the receptive endometrium and has a negative impact on implantation and IVF outcomes.

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References

  1. Paulson RJ, Sauer MV, Lobo RA (1990) Factors affecting implantation after human in vitro fertilization: a hypothesis. Am J Obstet Gynecol 163:2020–2023

    CAS  PubMed  Google Scholar 

  2. Donaghy M, Lessey BA (2007) Uterine receptivity: alternations associated with benign gynecologic disease. Semin Reprod Med 25:461–475

    Article  CAS  Google Scholar 

  3. Edwards RG (2006) Human implantation: the last barrier in assisted reproduction technologies? Reprod BioMed Online 13:887–904

    PubMed  Google Scholar 

  4. Dunn CL, Kelly RW, Critchley HO (2003) Decidualization of the human endometrial stromal cell: an enigmatic transformation. Reprod BioMed Online 7:151–161

    PubMed  Google Scholar 

  5. Dominquez F, Remohi J, Pellicer A, Simon C (2003) Human endometrial receptivity: a genomic approach. Reprod BioMed Online 6:332–338

    Google Scholar 

  6. Achache H, Revel A (2006) Endometrial receptivity markers, the journey to successful embryo implantation. Human Reprod Update 12:731–746

    Article  Google Scholar 

  7. Diedrich K, Fauser BC, Devroey P, Griesinger G, Evian Annual Reproduction (EVAR) workshop Group (2007) The role of the endometrium and embryo in human implantation. Human Reprod Update 13:365–377

    Article  CAS  Google Scholar 

  8. Acosta AA, Elberger L, Borghi M, Calamera JC, Chemes H, Doncel GF et al (2000) Endometrial dating and determination of the window of implantation in healthy fertile women. Fertil Steril 73:788–798

    Article  CAS  PubMed  Google Scholar 

  9. Bourgain C (2004) Endometrial biopsy in the evaluation of endometrial receptivity. J Gynecol Obstet Biol Reprod 33:S13–S17

    CAS  Google Scholar 

  10. Check JH, Choe JK, Katsoff D, Summers-Chase D, Wilson C (1999) Controlled ovarian hyperstimulation adversely affects implantation following in vitro fertilization-embryo transfer. J Assist Reprod Genet 16:416–420

    Article  CAS  PubMed  Google Scholar 

  11. Loeb L (1907) Uber die experimentelle erzeugung von knoten von deciduagewebe in dem uterus des meerschweinchens nach stattgefundener copulation. Z Allg Pathol Pathol Anat 18:563–565

    Google Scholar 

  12. Humphrey KW (1969) Interaction between estrogen-17 β and progesterone on the induction of deciduomata in ovariectomized mice. Aust J Exp Biol Med Sci 22:689–699

    CAS  Google Scholar 

  13. Finn CA, Martin L (1972) Endocrine control the timing of endometrial sensitivity to a decidual stimulus. Biol Reprod 7:82–86

    CAS  PubMed  Google Scholar 

  14. Barash A, Dekel N, Fieldust S, Segal I, Schechtman E, Granot I (2003) Local injury to the endometrium doubles the incidence of successful pregnancies in patients undergoing in vitro fertilization. Fertil Steril 79:1317–1322

    Article  PubMed  Google Scholar 

  15. Zhou L, Li R, Wang R, Huang H, Zhong K (2008) Local injury to the endometrium in controlled ovarian hyperstimulation cycles improves implantation rates. Fertil Steril 89:1166–1176

    Article  PubMed  Google Scholar 

  16. Abate V, De Corato R, Cali A, Stinchi A (1987) Endometrial biopsy at the time of embryo transfer: correlation of histological diagnosis with therapy and pregnancy rate. J In Vitro Fertil Embryo Transfer 4:173–176

    Article  CAS  Google Scholar 

  17. Ubaldi F, Bourgain C, Tournaye H et al (1997) Endometrial evaluation by aspiration biopsy on the day of oocyte retrieval in the embryo transfer cycles in patients with serum progesterone rise during the follicular phase. Fertil Steril 67:521–526

    Article  CAS  PubMed  Google Scholar 

  18. Akita S, Ishihara H, Mohammad Abdur R, Fujji T (2000) Leukemia inhibitory factor gene improves skin allograft survival in the mouse model. Transplantation 70:1026–1031

    Article  CAS  PubMed  Google Scholar 

  19. Sherer DM, Abulafia O (2001) Angiogenesis during implantation, and placental and early embryonic development. Placenta 22:1–13

    Article  CAS  PubMed  Google Scholar 

  20. Raziel A, Schachter M, Strassburger D, Bern O, Ron-El R, Friedler S (2007) Favorable influence of local injury to the endometrium in intracytoplasmic sperm injection patients with high-order implantation failure. Fertil Steril 87:198–201

    Article  PubMed  Google Scholar 

  21. Haouzi D, Mahmoud K, Fourar M, Bendhaou K, Dechaud H, De Vos J, Reme T, Dewailly D, Hamamah S (2009) Identification of new biomarkers of human endometrial receptivity in the natural cycle. Human Reprod 24:198–205

    Article  CAS  Google Scholar 

  22. Kalma Y, Granot I, Gnainsky Y, Or Y, Czernobilsky B, Dakel N, Barash A (2008) Endometrial biopsy-induced gene modulation: first evidence for the expression of bladder-transmembranal uroplakin lb in human endometrium. Fertil Steril (in press)

  23. van der Gaast MH, Beier-Hellwig K, Fauser BCJM, Beier HM, Macklon NS (2003) Endometrial secretion aspiration prior to embryo transfer does not reduce implantation rates. Reprod BioMed Online 7:105–109

    Article  PubMed  Google Scholar 

  24. Boomsma CM, Kavelaars A, Eijkemans MJC, Amarouchi K, Teklenburg G, Gutknecht D, Fauser BJCM, Heijnen CJ, Macklon NS (2009) Cytokine profiling in endometrial secretions: a non-invasive window on endometrial receptivity. Reprod BioMed Online 18:85–94

    CAS  PubMed  Google Scholar 

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Acknowledgments

The authors are grateful to the nursing and embryology staff of the Yazd Research and Clinical Center for Infertility for their assistance.

Conflict of interest statement

None.

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Authors and Affiliations

  1. Department of Obstetrics and Gynecology, Research and Clinical Center for Infertility, Shahid Sadoughi University of Medical Science, Bouali Ave, Safaieh, 8916877391, Yazd, Iran

    Mohammad Ali Karimzade, Homa Oskouian, Shahnaz Ahmadi & Leila Oskouian

Authors
  1. Mohammad Ali Karimzade
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  2. Homa Oskouian
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  3. Shahnaz Ahmadi
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  4. Leila Oskouian
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Corresponding author

Correspondence to Homa Oskouian.

Additional information

Clinicaltrials.gov ID: NCT00846183.

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Karimzade, M.A., Oskouian, H., Ahmadi, S. et al. Local injury to the endometrium on the day of oocyte retrieval has a negative impact on implantation in assisted reproductive cycles: a randomized controlled trial. Arch Gynecol Obstet 281, 499–503 (2010). https://doi.org/10.1007/s00404-009-1166-1

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  • DOI: https://doi.org/10.1007/s00404-009-1166-1

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