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Modulation of lipid fluidity likely contributes to the fructose/xylitol-induced acceleration of epidermal permeability barrier recovery

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Abstract

We previously showed that topical application of hexoses such as fructose accelerates barrier recovery after disruption. We also showed that various hexoses and polyols interact with phospholipid and alter the phase transition temperature. Thus, we hypothesized that the improvement of barrier recovery by hexoses and polyols might be related to the interaction with phospholipid. Here, we tested this idea by examining the effects of xylitol (a component of some skin-care products) and fructose on lipid dynamics in an epidermal-equivalent model at the single-cell level by means of two-photon microscopy after staining with Laurdan, a fluorescent dye sensitive to the physical properties of its membrane environment. First, we confirmed that topical application of xylitol aqueous solution on tape-stripped human skin accelerated barrier recovery. Then, we examined changes of lipid fluidity in the epidermal-equivalent model after application of water or an aqueous solution of xylitol or fructose. Application of xylitol and/or fructose increased the lipid fluidity in the uppermost part of the stratum granulosum layer, compared to treatment with water alone, and accelerated the exocytosis of lamellar bodies to the intercellular domain between stratum corneum and stratum granulosum. Our results support the idea that the improvement of epidermal barrier homeostasis upon topical application of xylitol or fructose is due to increased lipid fluidity in the uppermost layer of the stratum granulosum, which enables accelerated release of lipid from the stratum granulosum, thereby improving the lamellar structure and accelerating epidermal permeability barrier recovery.

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Acknowledgements

We appreciate the feedback offered by Dr. Makiko Goto (Shiseido Global Innovation Center). This work was supported by JST CREST Grant Number JPMJCR15D2, Japan.

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Authors and Affiliations

  1. Shiseido Global Innovation Center, Yokohama, Japan

    Yuki Umino & Mitsuhiro Denda

  2. Research Institute for Electronic Science, Hokkaido University, Sapporo, Japan

    Sari Ipponjima

  3. Shiseido Global Innovation Center, 2-2-1, Hayabuchi, Tsuzuki-ku, Yokohama, 224-8558 , Japan

    Yuki Umino

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  1. Yuki Umino
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  2. Sari Ipponjima
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  3. Mitsuhiro Denda
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Correspondence to Yuki Umino.

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The protocol was approved by the ethics committees of Shiseido Research Center, and was in accordance with the National Institute of Health guideline and Declaration of Helsinki principles.

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Umino, Y., Ipponjima, S. & Denda, M. Modulation of lipid fluidity likely contributes to the fructose/xylitol-induced acceleration of epidermal permeability barrier recovery. Arch Dermatol Res 311, 317–324 (2019). https://doi.org/10.1007/s00403-019-01905-0

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  • DOI: https://doi.org/10.1007/s00403-019-01905-0

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