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Changes in S100A8 expression in UV-irradiated and aged human skin in vivo

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Abstract

S100A8, a calcium-binding protein, is associated with keratinocyte differentiation, inflammation and wound healing. S100A8 is induced by various skin stresses and diseases, which suggests that S100A8 plays a role in those processes. However, it has not been reported how the expression of S100A8 is affected during skin aging or whether S100A8 plays a role in the skin aging process. In this study, we investigated the changes in S100A8 mRNA and protein following acute UV irradiation to human buttock skin and by intrinsic aging and photoaging in human sun-protected (upper-inner arm) and sun-exposed (forearm) skin of elderly subjects. Real-time PCR, western blot and immunohistochemical staining analyses of UV-irradiated young buttock skin revealed that S100A8 protein expression was increased at 24 h (3.0-fold) and 48 h (4.4-fold) after UV irradiation. S100A8 mRNA and protein were more highly expressed by 2.3- and 4.0-fold, respectively, in the sun-protected skin of elderly people than in that of young people. In addition, the sun-exposed skin of elderly expressed more S100A8 mRNA and protein than the sun-protected skin of the same individuals. In immunohistochemical staining, facial (photoaged) skin ≥72 years showed higher epidermal expression of S100A8 than that of the other age groups. Based on the above results, our data suggest that the expression of S100A8 is affected by acute UV irradiation, intrinsic aging and photoaging processes.

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References

  1. Benoit S, Toksoy A, Ahlmann M, Schmidt M, Sunderkotter C, Foell D, Pasparakis M, Roth J, Goebeler M (2006) Elevated serum levels of calcium-binding S100 proteins A8 and A9 reflect disease activity and abnormal differentiation of keratinocytes in psoriasis. Br J Dermatol 155:62–66. doi:10.1111/j.1365-2133.2006.07198.x

    Article  PubMed  CAS  Google Scholar 

  2. Bhattacharya S, Bunick CG, Chazin WJ (2004) Target selectivity in EF-hand calcium binding proteins. Biochim Biophys Acta 1742:69–79. doi:10.1016/j.bbamcr.2004.09.002

    Article  PubMed  CAS  Google Scholar 

  3. Broome AM, Ryan D, Eckert RL (2003) S100 protein subcellular localization during epidermal differentiation and psoriasis. J Histochem Cytochem 51:675–685

    PubMed  CAS  Google Scholar 

  4. Chung JH, Seo JY, Choi HR, Lee MK, Youn CS, Rhie G, Cho KH, Kim KH, Park KC, Eun HC (2001) Modulation of skin collagen metabolism in aged and photoaged human skin in vivo. J Invest Dermatol 117:1218–1224. doi:10.1046/j.0022-202x.2001.01544.x

    Article  PubMed  CAS  Google Scholar 

  5. Di Nuzzo S, Sylva-Steenland RM, Koomen CW, de Rie MA, Das PK, Bos JD, Teunissen MB (2000) Exposure to UVB induces accumulation of LFA-1 + T cells and enhanced expression of the chemokine psoriasin in normal human skin. Photochem Photobiol 72:374–382. doi:10.1562/0031-8655(2000)072<0374:ETUIAO>2.0.CO;2

    Article  PubMed  CAS  Google Scholar 

  6. Donato R (2001) S100: a multigenic family of calcium-modulated proteins of the EF-hand type with intracellular and extracellular functional roles. Int J Biochem Cell Biol 33:637–668. doi:10.1016/S1357-2725(01)00046-2

    Article  PubMed  CAS  Google Scholar 

  7. Eckert RL, Broome AM, Ruse M, Robinson N, Ryan D, Lee K (2004) S100 proteins in the epidermis. J Invest Dermatol 123:23–33. doi:10.1111/j.0022-202X.2004.22719.x

    Article  PubMed  CAS  Google Scholar 

  8. Foell D, Frosch M, Sorg C, Roth J (2004) Phagocyte-specific calcium-binding S100 proteins as clinical laboratory markers of inflammation. Clin Chim Acta 344:37–51. doi:10.1016/j.cccn.2004.02.023

    Article  PubMed  CAS  Google Scholar 

  9. Gebhardt C, Nemeth J, Angel P, Hess J (2006) S100A8 and S100A9 in inflammation and cancer. Biochem Pharmacol 72:1622–1631. doi:10.1016/j.bcp.2006.05.017

    Article  PubMed  CAS  Google Scholar 

  10. Ghavami S, Kerkhoff C, Los M, Hashemi M, Sorg C, Karami-Tehrani F (2004) Mechanism of apoptosis induced by S100A8/A9 in colon cancer cell lines: the role of ROS and the effect of metal ions. J Leukoc Biol 76:169–175. doi:10.1189/jlb.0903435

    Article  PubMed  CAS  Google Scholar 

  11. Gilchrest BA (1982) Age-associated changes in the skin. J Am Geriatr Soc 30:139–143

    PubMed  CAS  Google Scholar 

  12. Gilchrest BA (1989) Skin aging and photoaging: an overview. J Am Acad Dermatol 21:610–613. doi:10.1016/S0190-9622(89)70227-9

    Article  PubMed  CAS  Google Scholar 

  13. Grimbaldeston MA, Geczy CL, Tedla N, Finlay-Jones JJ, Hart PH (2003) S100A8 induction in keratinocytes by ultraviolet A irradiation is dependent on reactive oxygen intermediates. J Invest Dermatol 121:1168–1174. doi:10.1046/j.1523-1747.2003.12561.x

    Article  PubMed  CAS  Google Scholar 

  14. Hsu K, Passey RJ, Endoh Y, Rahimi F, Youssef P, Yen T, Geczy CL (2005) Regulation of S100A8 by glucocorticoids. J Immunol 174:2318–2326

    PubMed  CAS  Google Scholar 

  15. Hummerich L, Muller R, Hess J, Kokocinski F, Hahn M, Furstenberger G, Mauch C, Lichter P, Angel P (2006) Identification of novel tumour-associated genes differentially expressed in the process of squamous cell cancer development. Oncogene 25:111–121

    PubMed  CAS  Google Scholar 

  16. Jenkins G (2002) Molecular mechanisms of skin ageing. Mech Ageing Dev 123:801–810. doi:10.1016/S0047-6374(01)00425-0

    Article  PubMed  CAS  Google Scholar 

  17. Kerkhoff C, Klempt M, Sorg C (1998) Novel insights into structure and function of MRP8 (S100A8) and MRP14 (S100A9). Biochim Biophys Acta 1448:200–211. doi:10.1016/S0167-4889(98)00144-X

    Article  PubMed  CAS  Google Scholar 

  18. Lavker RM (1979) Structural alterations in exposed and unexposed aged skin. J Invest Dermatol 73:59–66. doi:10.1111/1523-1747.ep12532763

    Article  PubMed  CAS  Google Scholar 

  19. Lavker RM, Kligman AM (1988) Chronic heliodermatitis: a morphologic evaluation of chronic actinic dermal damage with emphasis on the role of mast cells. J Invest Dermatol 90:325–330. doi:10.1111/1523-1747.ep12456193

    Article  PubMed  CAS  Google Scholar 

  20. Lee YS, Sohn KC, Jang S, Lee Y, Hwang C, Kim KH, Cho MJ, Kim CD, Lee JH (2008) Anti-apoptotic role of S100A8 in X-ray irradiated keratinocytes. J Dermatol Sci 51:11–18. doi:10.1016/j.jdermsci.2008.01.003

    Article  PubMed  CAS  Google Scholar 

  21. Liu H, Huang K, Wu Y, Lin N, Li J, Tu Y (2007) The expression of interleukin-22 and S100A7, A8, A9 mRNA in patients with psoriasis vulgaris. J Huazhong Univ Sci Technol Med Sci = Hua zhong ke ji da xue xue bao Yi xue Ying De wen ban = Huazhong keji daxue xuebao 27:605–607

    Google Scholar 

  22. Ma W, Wlaschek M, Tantcheva-Poor I, Schneider LA, Naderi L, Razi-Wolf Z, Schuller J, Scharffetter-Kochanek K (2001) Chronological ageing and photoageing of the fibroblasts and the dermal connective tissue. Clin Exp Dermatol 26:592–599. doi:10.1046/j.1365-2230.2001.00905.x

    Article  PubMed  CAS  Google Scholar 

  23. Madsen P, Rasmussen HH, Leffers H, Honore B, Celis JE (1992) Molecular cloning and expression of a novel keratinocyte protein (psoriasis-associated fatty acid-binding protein [PA-FABP]) that is highly up-regulated in psoriatic skin and that shares similarity to fatty acid-binding proteins. J Invest Dermatol 99:299–305. doi:10.1111/1523-1747.ep12616641

    Article  PubMed  CAS  Google Scholar 

  24. Oikarinen A (1990) The aging of skin: chronoaging versus photoaging. Photodermatol Photoimmunol Photomed 7:3–4

    PubMed  CAS  Google Scholar 

  25. Passey RJ, Xu K, Hume DA, Geczy CL (1999) S100A8: emerging functions and regulation. J Leukoc Biol 66:549–556

    PubMed  CAS  Google Scholar 

  26. Rittie L, Fisher GJ (2002) UV-light-induced signal cascades and skin aging. Ageing Res Rev 1:705–720. doi:10.1016/S1568-1637(02)00024-7

    Article  PubMed  CAS  Google Scholar 

  27. Rundhaug JE, Hawkins KA, Pavone A, Gaddis S, Kil H, Klein RD, Berton TR, McCauley E, Johnson DG, Lubet RA, Fischer SM, Aldaz CM (2005) SAGE profiling of UV-induced mouse skin squamous cell carcinomas, comparison with acute UV irradiation effects. Mol Carcinog 42:40–52. doi:10.1002/mc.20064

    Article  PubMed  CAS  Google Scholar 

  28. Ryckman C, Vandal K, Rouleau P, Talbot M, Tessier PA (2003) Proinflammatory activities of S100: proteins S100A8, S100A9, and S100A8/A9 induce neutrophil chemotaxis and adhesion. J Immunol 170:3233–3242

    PubMed  CAS  Google Scholar 

  29. Seo JY, Lee SH, Youn CS, Choi HR, Rhie GE, Cho KH, Kim KH, Park KC, Eun HC, Chung JH (2001) Ultraviolet radiation increases tropoelastin mRNA expression in the epidermis of human skin in vivo. J Invest Dermatol 116:915–919. doi:10.1046/j.1523-1747.2001.01358.x

    Article  PubMed  CAS  Google Scholar 

  30. Sugiura H, Ebise H, Tazawa T, Tanaka K, Sugiura Y, Uehara M, Kikuchi K, Kimura T (2005) Large-scale DNA microarray analysis of atopic skin lesions shows overexpression of an epidermal differentiation gene cluster in the alternative pathway and lack of protective gene expression in the cornified envelope. Br J Dermatol 152:146–149. doi:10.1111/j.1365-2133.2005.06352.x

    Article  PubMed  CAS  Google Scholar 

  31. Thorey IS, Roth J, Regenbogen J, Halle JP, Bittner M, Vogl T, Kaesler S, Bugnon P, Reitmaier B, Durka S, Graf A, Wockner M, Rieger N, Konstantinow A, Wolf E, Goppelt A, Werner S (2001) The Ca2+-binding proteins S100A8 and S100A9 are encoded by novel injury-regulated genes. J Biol Chem 276:35818–35825. doi:10.1074/jbc.M104871200

    Article  PubMed  CAS  Google Scholar 

  32. Viemann D, Barczyk K, Vogl T, Fischer U, Sunderkotter C, Schulze-Osthoff K, Roth J (2007) MRP8/MRP14 impairs endothelial integrity and induces a caspase-dependent and -independent cell death program. Blood 109:2453–2460. doi:10.1182/blood-2006-08-040444

    Article  PubMed  CAS  Google Scholar 

  33. Yui S, Nakatani Y, Hunter MJ, Chazin WJ, Yamazaki M (2002) Implication of extracellular zinc exclusion by recombinant human calprotectin (MRP8 and MRP14) from target cells in its apoptosis-inducing activity. Mediators Inflamm 11:165–172. doi:10.1080/09622935020138208

    Article  PubMed  CAS  Google Scholar 

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Acknowledgments

This research was supported by a grant (R11-2002-097-01001-2) through the Center for Aging and Apoptosis Research at Seoul National University from the Korean Science and Engineering Foundation (KOSEF), a grant funded by the Korea government (MEST), and a grant from the Amore-Pacific Corporation.

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Authors and Affiliations

  1. Department of Dermatology, Seoul National University College of Medicine and Laboratory of Cutaneous Aging Research, Clinical Research Institute, Seoul National University Hospital, 28, Yeongeon-Dong, Chongno-Gu, Seoul, 110-744, Korea

    Young Mee Lee, Yeon Kyung Kim, Hee Chul Eun & Jin Ho Chung

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  1. Young Mee Lee
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Correspondence to Jin Ho Chung.

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Lee, Y.M., Kim, Y.K., Eun, H.C. et al. Changes in S100A8 expression in UV-irradiated and aged human skin in vivo. Arch Dermatol Res 301, 523–529 (2009). https://doi.org/10.1007/s00403-009-0960-8

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