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Gene expression profiling reveals alteration of caspase 6 and 14 transcripts in normal skin of keloid-prone patients

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Abstract

Excessive scar formation in keloids points to altered tissue modeling and repair mechanisms. Dysregulation of cytokine and apoptotic cascades and their downstream signaling pathways might have a role in keloid development. Total RNA was isolated from biopsied keloidal tissue and adjacent normal skin of black patients, white patient’s scars, and normal skin of black and white patients, with normal wound healing. Apoptosis, cytokine and NFkB pathway microarrays were used to study and compare gene expression levels. Real-time PCR was used to verify microarray results in original samples and a separate, validation-set of samples. Significant differences were observed in the expression levels of members of caspase, cytokines and MAP kinase pathways, between the normal skin of keloid-prone and normal skin of keloid-resistant patients. Specifically, expression of caspase 6, and caspase 14 genes were different between normal skin of keloid-prone individuals and normal skin of keloid-resistant patients. Our results suggest that normal skin of keloid-prone individuals constitutively expresses a distinct gene profile which might contribute to their susceptibility to develop keloids.

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Authors and Affiliations

  1. Department of Pathology, Laboratory Medicine, Indiana University School of Medicine, Clarian Pathology Laboratory 350 W. 11th Street CPL 5044, Indianapolis, IN, 46202, USA

    Mehdi Nassiri

  2. Department of Pathology, University of Miami Miller School of Medicine, 1600 NW 10th Ave., Rosenstiel Medical Science Building, Room 7023, Miami, FL, 33136, USA

    Sharon Ramos, Dijana Gugic & Anita Viciana

  3. Department of Dermatology, University of Miami Miller School of Medicine, Miami, FL, USA

    Heather Woolery-Lloyd, Sharon E. Jacob, Paolo Romanelli, George Elgart & Brian Berman

  4. Department of Pathology, University of Florida, Gainesville, FL, USA

    Vladimir Vincek

Authors
  1. Mehdi Nassiri
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  2. Heather Woolery-Lloyd
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  3. Sharon Ramos
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  4. Sharon E. Jacob
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  5. Dijana Gugic
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  6. Anita Viciana
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  7. Paolo Romanelli
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  8. George Elgart
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  9. Brian Berman
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  10. Vladimir Vincek
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Correspondence to Mehdi Nassiri.

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Nassiri, M., Woolery-Lloyd, H., Ramos, S. et al. Gene expression profiling reveals alteration of caspase 6 and 14 transcripts in normal skin of keloid-prone patients. Arch Dermatol Res 301, 183–188 (2009). https://doi.org/10.1007/s00403-008-0880-z

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  • DOI: https://doi.org/10.1007/s00403-008-0880-z

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