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Expression of nitric oxide synthases in keratinocytes after UVB irradiation

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Abstract

The importance of nitric oxide (NO) in mediating vasodilation, neurotransmission, and immune and inflammatory responses has been demonstrated. Human keratinocyte express inducible nitric oxide synthase (iNOS) and the neuronal constitutive isoform of NOS (ncNOS). We established an in vitro model in keratinocytes to investigate changes in NO, iNOS and ncNOS expression after UVB exposure. We demonstrated a large induction of NO after UVB exposure and that the source of NO produced in UVB-exposed keratinocytes was increased expression of iNOS and ncNOS. The increased NO production with increased expression of iNOS and ncNOS may contribute to the pathological and physiological features of UVB-induced erythema and skin inflammation.

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Authors and Affiliations

  1. Department of Biomedical Engineering, I-Shou University, 1, Section 1, Shiuecheng Road, Dashu Shiang, Kaohsiung County, Taiwan

    Huoy-Rou Chang

  2. Department of Medical Technology, Fooyin University of Technology, Taiwan

    Der-An Tsao

  3. Department of Medical Chemistry, Chia-Nan University of Pharmacy and Science, Taiwan

    Shiow-Ru Wang

  4. Department of Dermatology, College of Medicine, Taiwan University, Taiwan

    Hsin-Su Yu

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  1. Huoy-Rou Chang
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  2. Der-An Tsao
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  3. Shiow-Ru Wang
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  4. Hsin-Su Yu
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Correspondence to Huoy-Rou Chang.

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Chang, HR., Tsao, DA., Wang, SR. et al. Expression of nitric oxide synthases in keratinocytes after UVB irradiation. Arch Dermatol Res 295, 293–296 (2003). https://doi.org/10.1007/s00403-003-0433-4

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  • DOI: https://doi.org/10.1007/s00403-003-0433-4

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