Abstract
Microtubule-associated protein tau (MAPT) aggregates in neurons, astrocytes and oligodendrocytes in a number of neurodegenerative diseases, including progressive supranuclear palsy (PSP). Tau is a target of therapy and the strategy includes either the elimination of pathological tau aggregates or reducing MAPT expression, and thus the amount of tau protein made to prevent its aggregation. Disease-associated tau affects brain regions in a sequential manner that includes cell-to-cell spreading. Involvement of glial cells that show tau aggregates is interpreted as glial cells taking up misfolded tau assuming that glial cells do not express enough MAPT. Although studies have evaluated MAPT expression in human brain tissue homogenates, it is not clear whether MAPT expression is compromised in cells accumulating pathological tau. To address these perplexing aspects of disease pathogenesis, this study used RNAscope combined with immunofluorescence (AT8), and single-nuclear(sn) RNAseq to systematically map and quantify MAPT expression dynamics across different cell types and brain regions in controls (n = 3) and evaluated whether tau cytopathology affects MAPT expression in PSP (n = 3). MAPT transcripts were detected in neurons, astrocytes and oligodendrocytes, and varied between brain regions and within each cell type, and were preserved in all cell types with tau aggregates in PSP. These results propose a complex scenario in all cell types, where, in addition to the ingested misfolded tau, the preserved cellular MAPT expression provides a pool for local protein production that can (1) be phosphorylated and aggregated, or (2) feed the seeding of ingested misfolded tau by providing physiological tau, both accentuating the pathological process. Since tau cytopathology does not compromise MAPT gene expression in PSP, a complete loss of tau protein expression as an early pathogenic component is less likely. These observations provide rationale for a dual approach to therapy by decreasing cellular MAPT expression and targeting removal of misfolded tau.
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Introduction
Tau is a microtubule-associated protein encoded by the MAPT gene, which was initially described as a neuronal protein that is enriched in axons and has important functions in binding and stabilizing the cytoskeleton and regulating cellular transport [26, 68]. Recent studies also highlight that tau interacts with other cellular structures including cytoplasmic organelles, plasma membrane, the actin cytoskeleton, nucleus and dendrites [2, 8, 30, 32, 61, 62]. Tau is the most commonly deposited protein in the ageing brain and in neurodegenerative diseases, including progressive supranuclear palsy (PSP), where it aggregates in neurons, oligodendrocytes and astrocytes [16, 17]. The hallmark pathological features of PSP include neurofibrillary tangles (NFTs) in neurons in subcortical and brainstem regions in combination with tau-immunopositive tufted astrocytes [56]. Tau-immunopositive oligodendrocytic coiled bodies are also a common and widespread neuropathological feature of PSP. Tau is a major therapeutic target for PSP and other tau-depositing neurodegenerative diseases, which are currently focused on the elimination of pathological aggregates from the brain or directly targeting MAPT gene expression with antisense oligonucleotide (ASO) therapies to inhibit tau aggregation [5, 34, 52, 57, 63].
Disease-associated tau affects brain regions in a hierarchical and sequential manner that includes cell-to-cell spreading, which is well described for neurons [20, 57, 67]. Involvement of glial cells showing fibrillar tau aggregates has been interpreted as indicating that glial cells internalize disease-associated tau released from neurons [35]. These concepts assume that glial cells do not express substantial amounts of MAPT. Although several studies have evaluated MAPT expression in tissue homogenates [6, 24, 29, 47, 65, 66], in humans there is a paucity of studies focusing on MAPT gene expression in glia. Early in situ hybridization studies that mapped the distribution of tau mRNA in the cerebral cortex and hippocampus of adult human brain showed that tau mRNA is expressed in neurons and is absent in glia [23]. However, oligodendrocytes have an elaborate microtubule network, which provide the tracks for organelle trafficking and intracellular translocation of myelin gene products, suggesting that tau may have functions in regulating microtubule-dependent processes as it does in neurons [31]. Low levels of tau mRNA have been identified in human oligodendrocytes (http://www.brainrnaseq.org) [71] and low levels of MAPT expression in cultured oligodendrocytes [45, 46, 51, 58], as well as astrocytes and oligodendrocytes in online databases [48]. A recent study that used tauGFP knock-in/knock-out mice with an eGFP-coding sequence inserted into the first exon of the MAPT gene generating cytoplasmic eGFP expression under the endogenous tau promoter reported endogenous expression only in oligodendrocytes and neurons [66]. These observations are consistent with the detection of tau protein in oligodendrocytes using Tau5 immunohistochemistry [46]. In contrast, limited studies suggest that tau might be expressed in astrocytes. For example, tau mRNA is expressed in low amounts in astrocytes (http://www.brainrnaseq.org) [71] and has been reported in astrocytic tumours [49]. Tau-immunostaining has also been reported in astrocytes in the normal human brain and in astrocytic tumours [49, 59]. Despite these studies, the prevailing view is that glia do not express or contain sufficient amounts of MAPT mRNA and tau protein to build fibrillar inclusions independently in response to an unidentified neurodegeneration-inducing event in PSP and other tau-related neurodegenerative diseases [35, 38].
A number of neuropathological studies in humans have reported the presence of astrocytic or oligodendrocytic tau-immunopositive inclusions in the absence of neuronal tau [18, 37]. Further observations on the distinct sequential distribution of glial tau cytopathologies also raise the possibility of whether these can develop independently of neuronal tau [40, 41]. Post-translational modifications of tau have been examined in astrocytes and oligodendrocytes and show that tau in these cell types is phosphorylated at different sites and has an altered conformation [14]. These observations could suggest that glial cells have basic MAPT and tau protein expression that can be phosphorylated and fibrillized into a pathological inclusion, rather than tau being taken up from neurons.
Another uncertain aspect of disease pathogenesis is whether MAPT expression is compromised in cells accumulating phosphorylated pathological tau aggregates (i.e. a “loss-of-function” component to the pathogenesis). To address these perplexing aspects of disease pathogenesis, this study used RNAscope combined with immunostaining, complemented by single-nucleus (sn)RNA sequencing from PSP and control human brains, to systematically map and quantify MAPT expression in neurons, oligodendrocytes and astrocytes across different brain regions to determine whether there are cell type and/or regional differences in MAPT expression. This study also investigated whether MAPT gene expression is altered in neurons or glia containing tau-immunopositive inclusions in PSP.
Materials and methods
Cases and tissue collection
Brains from three control individuals without neurodegenerative pathology (3 male; mean age: 75 years) and three PSP patients who underwent medical assistance in dying (MAID; 3 male; mean age: 75 years) were included in this study. Participants were prospectively enrolled in longitudinal multidisciplinary research programmes and recruited with informed consent through local brain donor programmes. Demographic and genetic (MAPT haplotype) information was collected from an integrated clinicopathological database. All cases included in this study had a routine neuropathological assessment using standardized neuropathological consensus recommendations. The three control cases were selected based on their similar postmortem delay (4–12.5 h) to the PSP cases (3.5–14 h) included in this study.
RNAscope combined with immunostaining for AT8
To map the anatomical distribution of MAPT gene expression in different cell types throughout the brain, formalin-fixed paraffin-embedded 4 μm sections from the superior frontal cortex, hippocampus, striatum, midbrain and cerebellum from one control case were processed for RNAscope combined with AT8 immunoperoxidase and for RNAscope combined with AT8 immunofluorescence. To determine MAPT gene expression in cellular cytopathologies, formalin-fixed paraffin-embedded 4 μm sections from the superior frontal cortex and striatum from three PSP cases were processed for RNAscope combined with AT8 immunofluorescence. Sections were deparaffinized and rehydrated to perform the following RNAscope® in situ hybridization assay according to the manufacturer’s instructions (Advance Cell Diagnostics (ACD), Hayward, CA, USA). Sections were incubated with the RNAscope® hydrogen peroxide for 10 min at RT and were pre-treated in boiling RNAscope® 1X Target Retrieval Reagents solution for 15 min. Sections were then incubated in the RNAscope® Protease Plus solution for 30 min at 40 °C. Each section was hybridized with the MAPT probe (408991-C2) and one of the following cell type-specific probes: Olig2 (424191-C1), RBFOX3 (415591-C4) and ALDH1L1 (438881-C3), for 2 h at 40 °C. Signals were amplified using RNAScope® Multiplex FL v2 Amp 1,2, and 3 according to the manufacturer’s directions. Signals were then developed accordingly to the appropriate channels. Opal 570 dye (1:1500; Akoya Biosciences, Marlborough, MA, USA) was used to visualize MAPT probes and Opal 690 dye (1:1000) was used for visualization of the cell type-specific probes. Following a final wash, sections were proceeded to immunofluorescence staining. Briefly, sections were washed with DAKO washing buffer (Dako, Santa Clara, CA, USA), blocked with DAKO peroxidase solution for 10 min and incubated with anti-tau AT8 (Phospho-Tau, Ser202, Thr205; 1:1000; Thermo Fisher) for 1 h at RT, followed by the secondary antibody Alexa Fluor 488 donkey anti-mouse IgG (H + L) (1:500; Thermo Fisher, Rockford, IL, USA) for 1 h at RT in the dark. All sections were counterstained with DAPI to visualize cell nuclei and mounted with ProLong™ Gold Antifade Mountant (Thermo Fisher, Rockford, IL, USA). A similar protocol was used for RNAscope for light microscopy based on manufacturer’s instructions, using the same MAPT, RBFOX3, Olig2 and ALDH1L1 probes as above (https://acdbio.com/sites/default/files/RNAscope_Sample_Preparation_Pretreatment_Guide_FFPETissue.pdf), omitting AT8 immunostaining.
Morphometry
Images of RNAscope fluorescent sections were analysed using algorithms created on the General Analysis 3 module in NIS Elements software (version 5.30.04, Nikon Instruments Inc.). Cell types of interest were identified by capturing nuclei (DAPI) which were then extended in radius length corresponding to the size of cell types in the spatially defined regions and annotating those which contained positivity for cell type marker (RBFOX3, Olig2, ALDH1L1) probes within the defined cell area. Cell marker positivity was thresholded by fluorescence intensity and area to eliminate false detection in different cell types due to extending processes or proximity among cells. In the substantia nigra, cerebellum (Purkinje cells) and globus pallidus, neurons were manually annotated due to their distinct morphologies in respect to the nucleus. Annotated cells were converted into individual regions of interest for evaluation of MAPT transcripts in each cell. MAPT transcripts were observed individually or in small confluent clusters and for this reason, individual transcripts could not be counted with confidence. Therefore, in this study, we used the area density values of MAPT transcripts. This was calculated by the total area of MAPT transcripts divided by the annotated area for each cell and expressed as a percentage. To measure nuclear area density of MAPT transcripts in oligodendrocytes and astrocytes, a region of interest was manually drawn around the nucleus, identified with DAPI, in oligodendrocytes and astrocytes without tau-immunopositive inclusions in the basal ganglia of three PSP cases.
Single-nuclear (sn)RNAseq
Tissue was collected from the frontal cortex in three control and three PSP cases and flash frozen and stored at −80C for snRNAseq. Frozen brain tissue was chopped into smaller pieces on dry ice. Frozen tissues were immediately loaded on the Singulator TM 100 (S2 Genomics1). Nuclei isolation was operated by following the “Small Volume Nuclei Isolation Protocol” from the Automated Tissue Dissociation System with minor adjustments. The Singulator 100 instrument was primed with cold nuclei isolation (NIS) and storage buffers (NSR). Frozen tissues were loaded on the Singulator cartridge which was primed with a 40 µm filter. The cartridge was immediately mounted onto the system for nuclei isolation. All of the above materials were supplied by S2 Genomics. In addition, RNase inhibitor (Sigma-Aldrich; Cat # 3335402001) was added to the Singulator Cartridge to reach a final concentration of 0.2U/μl. Nuclei collected from the Singulator was spun at 800×g, at 4 °C for 10 min. The supernatant was removed, followed by resuspending nuclei in freshly made cold wash and resuspension buffer (1× PBS, 1% BSA, 0.2U/μl). The wash was repeated twice. Nuclei were stained with DAPI and sorted for DAPI-positive cells to exclude any debris or nuclei aggregates using an Aria Fusion A cell sorter. The nuclei were collected in wash and resuspension buffer and spun at 800×g for 10 min at 4 °C. The supernatant was removed, and then resuspended in wash and resuspension buffer. Nuclei were stained with SYBR Green II and counted under a microscope using INCYTO C-Chip Hemocytometer (Neubauer Improved).
Sorted nuclei were used as input into the 10× Genomics single-cell 3′ v3.1 assay and processed as described by the protocol provided by the 10X Genomics. Library construction and library sequencing proceeded as described in the 10X Genomics protocol. In brief, the molarity of each library was calculated based on library size as measured by bioanalyser (Agilent Technologies) and qPCR amplification data (Roche). Samples were pooled and normalized to 1.5 nM. Library pool was denatured using 0.2N NaOH (Sigma) for 8 min at room temperature and neutralized with 400 mM Tris–HCL (Sigma). Library pool at a final concentration of 300 pM were loaded to sequence on Novaseq 6000 (Illumina). Samples were sequenced with the following run parameters: Read 1–28 cycles, Read 2–90, index 1–10 cycles, index 2–10 cycles. Sequencing target read depth per library was ~ 40,000 reads per nuclei. snRNAseq was performed by the Princess Margaret Genomics Centre (Toronto, Canada).
Alignment, barcode assignment and UMI counting
Single-nuclei transcriptome sequencing data were pre-processed using the Cell Ranger Single-Cell Software Suite (v 6.0.0) (10X Genomics). Cell barcodes and UMI (unique molecular identifiers) barcodes were demultiplexed and single-end reads aligned to the reference genome, GRCh38, using the STAR (v 2.7.10) [11] tool of Cell Ranger pipeline. The resulting cell–gene matrix contains UMI counts by gene and by cell barcode. To proceed with the clustering analysis, three each (control, PSP) gene expression quantification experiments were merged into a single- feature-barcode matrix. ‘CellRanger aggr’ runs depth normalization algorithm to make all merged datasets have a similar number of uniquely mapped transcriptome reads per cell.
Clustering and differential gene expression analysis
Dimensionality reduction was performed by running principal component analysis (PCA). Ten PCs were retained for the analysis and cells were clustered based on k-means algorithm, visualized using UMAP (uniform manifold approximation and projection). We assessed cluster-specific marker genes in PSP and control clusters by detecting the differentially expressed genes between the given cluster and the other clusters (p < 0.05; negative binomial exact test).
Cell type annotation
The cell types were annotated by mapping the known brain cell type markers [28] to the differentially expressed marker genes in each cluster. We employed stringent criteria where three approaches were used to assign cell type identity to each cluster [53, 54]. First, we plotted the number of overlapping genes in known marker genes and cluster differentially expressed genes (top 50 genes in each cluster, based on log2 fold change). Second, we plotted the mean expression of differentially expressed marker genes across all clusters. Third, we quantified the mean expression of the known marker genes across clusters. Heatmaps and boxplots were generated using the plotly package in Python. Cell type identity was assigned to each cluster based on the restricted expression of marker genes (combining three approaches).
MAPT gene expression
Control and PSP expression matrices were normalized to 10,000 reads per cell and transformed using log2 (expression) using Scanpy (v 1.7.2). These values were used to quantify MAPT expression across different cell types in controls and PSP.
Statistical evaluation
SPSS Statistics version 23 and Prism (version 9.5.1) were used for statistical analyses. Mann–Whitney U tests were used to compare the difference in MAPT gene expression between control and PSP groups in snRNAseq studies. For RNAscope studies, normality of the area density of MAPT gene transcripts in neurons, oligodendrocytes and astrocytes with and without tau-immunopositive inclusions was tested using the Kolmogorov–Smirnov test [10] and p values < 0.05 were regarded as having a non-normal distribution. Mann–Whitney U and Kruskal–Wallis tests were used to compare the area density of MAPT gene transcripts in neurons, oligodendrocytes and astrocytes with and without tau-immunopositive inclusions, and between groups. The standard significance level was set at 0.01. The z-score (standard score) cutoff value was used as an additional conservative approach [10] to determine the proportion (%) of cells with tau-immunopositive inclusions with area density values above or below the 95% percentile of the area density values of cells without tau-immunopositive inclusions. If this proportion reached 50% of cells, we interpret this as a strong indicator for an increase or decrease of MAPT transcript area density compared with cells without tau-immunopositive inclusions. Area density is reported as the mean ± standard error.
Results
Demographics and characterization of cases examined
Demographic and neuropathological data of patients used in this study are summarized in Table 1. Controls ranged from 74 to 77 years at death, and PSP cases ranged from 73 to 77 years at death. All cases used in this study had an absence or low AD neuropathological change. All PSP cases were H1/H1 MAPT haplotype.
Light microscopy RNAscope
MAPT transcripts revealed by RNAscope in controls showed expression in neurons, oligodendrocytes and astrocytes in all brain regions examined (Fig. 1). The cell-specific transcripts, RBFOX3, Olig2 and ALDH1L1, were observed in the nuclei and cytoplasm of neurons, oligodendrocytes and astrocytes, respectively. In neurons, MAPT transcripts were observed in both the nucleus and cytoplasm, individually or in clusters of transcripts (Fig. 1a, b). In oligodendrocytes and astrocytes, MAPT transcripts were observed as individual transcripts and located in the nucleus and cytoplasm (Fig. 1c, d).
Fluorescence RNAscope combined with immunostaining for AT8
In the control case, similar to the light microscopy RNAscope, MAPT transcripts revealed by fluorescent RNAscope were observed in neurons, oligodendrocytes and astrocytes in all brain regions examined as confirmed with the cell-specific transcripts, RBFOX3, Olig2 and ALDH1L1, respectively (Figs. 2 and 3). Within each cell type, a similar pattern of MAPT transcripts was observed in the cytoplasm and nucleus as described above. MAPT transcripts varied within neurons, oligodendrocytes and astrocytes, and between brain regions for each cell type. RBFOX3 transcripts predominated in the cytoplasm compared to the nucleus of neurons. A high density of RBFOX3 transcripts was observed in the hippocampal dentate gyrus and cerebellar granule cell layer, corresponding to the high density of neurons in these regions (Fig. 2c, f). Olig2 and ALDH1L1 transcripts in oligodendrocytes and astrocytes (Fig. 3), respectively, were found in the cytoplasm and nucleus. Distinct islands with a high density of Olig2 transcripts were observed in the pencil fibres of Wilson, corresponding to the high density of oligodendrocytes in these fibre bundles compared with the surrounding grey matter (Fig. 3a). MAPT transcripts were not detected in the Schwann cells of the extraneural segment of the oculomotor nerve and only occasional MAPT transcripts were seen in the axons of the oculomotor nerve (Online Resource 1). Importantly, MAPT, RBFOX3, Olig2 and ALDH1L1 transcripts were not observed in endothelial cells (Online Resource 2). AT8-immuopositive inclusions were not observed in the control case.
In neurons, the highest area density of MAPT transcripts was found in the frontal and entorhinal cortices (21.7 and 21.3%, respectively), followed by the temporal cortex, hippocampal CA1 region, basal ganglia and other subcortical regions (2.6–7.6%). Purkinje neurons had the lowest area density of MAPT transcripts (1.2 ± 0.1%; Fig. 4a). Pooling neurons from all brain regions revealed an overall area density of 7.2 ± 0.5% of MAPT transcripts in neurons. In oligodendrocytes, the highest area density of MAPT transcripts was found in the frontal white matter (4.4 ± 0.5%) and lowest in the cerebellar white matter (< 1.0%; Fig. 4b). In astrocytes, a similar area density of MAPT transcripts was found across all brain regions examined (1.7–4.5%; Fig. 4c). Overall, pooling each cell type from all brain regions revealed that neurons had the highest area density of MAPT transcripts (7.2 ± 0.5%; p < 0.001), compared with oligodendrocytes (3.1 ± 0.2%) and astrocytes (3.1 ± 0.2%).
In PSP cases (n = 3), the presence of MAPT transcripts was confirmed in cells that contained tau-immunopositive inclusions (Fig. 4d–f), including NFTs (Fig. 5a–d), oligodendrocytic coiled bodies (Fig. 6a–d) and tufted astrocytes (Fig. 6e–g). Similar to cells without tau-immunopositive inclusions, the number of MAPT transcripts in cells with NFTs, coiled bodies and tufted astrocytes varied.
Quantification of MAPT transcripts in PSP using fluorescence RNAscope
For each cell type with and without tau-immunopositive inclusions, the pooled area density of MAPT transcripts in both the frontal cortex and basal ganglia followed a non-normal distribution pattern, which was confirmed by Kolmogorov–Smirnov test (pooled neurons: p < 0.001; pooled NFTs: p < 0.001; pooled oligodendrocytes: p < 0.001; pooled coiled bodies: p < 0.001; pooled astrocytes: p < 0.001; pooled tufted astrocytes: p < 0.001). Only the MAPT transcript area density in NFTs in the frontal cortex showed a normal distribution on Kolmogorov–Smirnov test (p = 0.225). Similarly, MAPT transcript area density in each cell type with and without tau-immunopositive inclusions followed a non-normal distribution pattern in each region, the frontal cortex and basal ganglia (all p < 0.001).
Similar to the control case (Fig. 4), in PSP cases MAPT transcript area density differed between cell types without tau-immunopositive inclusions (p < 0.001). For MAPT transcript area density in cells without tau-immunopositive inclusions, 61% (440/866) of neurons were above the 95% percentile of the Z-score of oligodendrocytic MAPT transcript area density and 56% (485/866) of neurons were above the 95% percentile of the Z-score of astrocytic MAPT transcript area density.
Next, we evaluated nuclear MAPT transcript area density in oligodendrocytes and astrocytes without tau-immunopositive inclusions in PSP cases. MAPT transcript area density in the nucleus differed between oligodendrocytes and astrocytes (p < 0.001). Astrocytes had a higher MAPT area density in the nucleus (3.8 ± 0.2%, n = 264 cells from 3 PSP cases) compared with oligodendrocytes (2.6 ± 0.1%, n = 333 cells from 3 PSP cases).
In PSP cases, the area density of MAPT transcripts was compared in neurons, oligodendrocytes and astrocytes with and without tau-immunopositive inclusions within cases, and between brain regions (Figs. 4 and 7). Due to the large variation in area density of MAPT transcripts in all cell types with and without tau-immunopositive inclusions, the number of neurons, oligodendrocytes and astrocytes with tau-immunopositive inclusions that were above the z-score (95% percentile) of neurons, oligodendrocytes and astrocytes without tau-immunopositive inclusions was also determined. There was a similar MAPT area density in neurons without tau-immunopositive inclusions and those containing NFTs. Higher MAPT area density was observed in NFT-containing neurons (p < 0.001) in the basal ganglia in one PSP case only. For MAPT area density, only 5% (3/36) of neurons with NFTs were above the 95% percentile Z-score of neurons without tau-immunopositive inclusions. Comparing between brain regions, only one NFT-containing neuron in the frontal cortex (1/30, 3%) and three neurofibrillary-containing neurons in the basal ganglia (3/34, 9%) were above the 95% percentile Z-score cutoff of MAPT transcript area density in neurons without tau-immunopositive inclusions.
Overall, the area density of MAPT transcripts in coiled bodies was increased compared with oligodendrocytes without tau-immunopositive inclusions. In the frontal cortex, higher area density of MAPT transcripts was observed in coiled bodies in one case (p = 0.02), and in the basal ganglia, higher MAPT area density was observed in coiled bodies in all cases (Case 1 p = 0.002, Cases 2 and 3 p < 0.001) compared with oligodendrocytes without tau-immunopositive inclusions. Pooling data for all cases (p < 0.001) and for each brain region (frontal cortex p = 0.002; basal ganglia p < 0.001) showed an increase in area density of MAPT transcripts in coiled bodies. However, due to the large variation in MAPT area density in all oligodendrocytes, the number of coiled bodies that were above the Z-score cutoff (95% percentile) of oligodendrocytes without tau-immunopositive inclusions was determined. For MAPT area density in oligodendrocytes, 16% (18/112) of coiled bodies were above the Z-score of oligodendrocytes without tau-immunopositive inclusions. Comparing between brain regions, 11% (3/27) and 20% (17/85) of coiled bodies in the frontal cortex and basal ganglia, respectively, were above the Z-score cutoff of MAPT area density of oligodendrocytes without tau-immunopositive inclusions.
Overall, the MAPT transcript area density in tufted astrocytes varied and was increased in the frontal cortex in two cases (Case 1 p = 0.005, Case 2 p = 0.001), and in one case in the basal ganglia (p = 0.02). However, similar MAPT transcript area density in astrocytes with and without tau-immunopositive astrocytes was found between brain regions. For MAPT transcript area density in astrocytes, 13% (5/36) tufted astrocytes were above the 95% percentile Z-score of astrocytes without tau-immunopositive inclusions. Comparing between brain regions, 12% (3/25) and 18% (2/11) of tufted astrocytes in the frontal cortex and basal ganglia, respectively, were above the 95% percentile Z-score cutoff of MAPT area density of astrocytes without tau-immunopositive inclusions.
Regional MAPT gene expression in CNS cell populations
We performed snRNAseq to obtain the transcriptome of cells isolated from freshly frozen brain samples. 31,936 single-nuclei transcriptome profiles including 15,265 from control and 16,671 from PSP cases were generated, and a median UMI count of 8007 per cell (2998 median genes per cell) and 6374 counts per cell (2894 median genes per cell) was detected, respectively. The libraries were sequenced to 88.8% and 89.2% average saturation in control and PSP samples, respectively. We clustered the control and PSP samples together, and the clusters were annotated according to the expression of known cell type marker genes. For example, oligodendrocytes comprised the largest cell cluster, representing 68% and 35% of all cells in controls and PSP, respectively (Fig. 8a, b).
Similar to the RNAscope, snRNAseq data showed that the MAPT gene expression varied between and within cell types in the frontal cortex in control cases (n = 3; Fig. 8c, d). The number of cell types captured within each cluster varied and for this reason, MAPT gene expression was normalized to the number of cells. The highest MAPT gene expression was found in inhibitory neurons, followed by astrocytes and oligodendrocyte precursor cells (OPCs). Low levels of MAPT gene expression were found in excitatory neurons and mature oligodendrocytes, and MAPT was almost absent from endothelial cells.
Cell type- specific changes in MAPT gene in PSP brain
Single-cell data revealed that MAPT gene expression was less in inhibitory neurons, astrocytes and OPCs in PSP cases (p < 0.0001 for all, n = 3) than in controls. Mature oligodendrocytes and excitatory neurons contained similar levels of MAPT gene expression in controls and PSP (Fig. 8e).
Discussion
This study mapped MAPT mRNA expression and compared it with tau protein pathology on a cellular level in the human brain. Implications of our study can be summarized as follows: (i) we show with different methods that in addition to neurons, MAPT mRNA is consistently expressed in oligodendrocytes and astrocytes; (ii) cellular MAPT expression varies between and within cell types even in the same anatomical area; and finally, (iii) MAPT expression is preserved in neurons and glia containing pathological tau protein aggregates.
Tau is the most frequent neurodegenerative disease-associated protein showing fibrillar aggregates in neurons, astrocytes and oligodendrocytes in a number of neurodegenerative diseases. PSP is one major tauopathy that represents all three cytopathologies. Tau pathology in PSP shows sequential involvement of brain regions involving neurons, astrocytes and oligodendrocytes, each with a distinct pattern of hierarchical involvement [41]. Cell-to-cell spreading is discussed for tau and thought to occur via similar mechanisms for neurons and glia [19, 21, 33, 55]. Since glial cells show fibrillar tau aggregates [17] and MAPT expression has been assumed to be absent or barely detectable (http://www.brainrnaseq.org) [23, 71], the origin of tau protein pathology in glial cells has been considered to be a consequence of an uptake of released neuronal disease-associated tau.
Neurons have a well-described maturation process of tau protein deposits, which include early biochemical changes, tau aggregation (pre-tangles), fibrillization in ubiquitinated neurofibrillary tangles (NFTs) and eventually extracellular (ghost) NFTs once the neuron has degenerated [4]. A similar process has been suggested for astrocytic tau inclusions [38]. Neurons contain MAPT transcripts, which serve as a local pool for increased protein production. In addition, neurons also uptake misfolded tau from the extracellular space, which is released as tau seeds from surrounding and/or synaptically and functionally interconnected neurons containing misfolded tau aggregates [21]. This is likely to lead to a facilitated aggregation of misfolded tau from both sources. Based on literature data and our observations, we propose two mechanistic scenarios for cell-to-cell transmission and accumulation of tau-immunopositive inclusions in oligodendrocytes and astrocytes leading to hierarchical distribution patterns of pathology (Fig. 9). In both scenarios, the constantly available cellular pool of tau allows it to reach a critical concentration to initiate or facilitate an aggregation process [66]. In scenario 1, following maturation of protein deposits in neurons, tau seeds released into the extracellular space are taken up by oligodendrocytes and/or astrocytes via a variety of yet unidentified mechanisms. Astrocytes have been shown to express phagocytic receptors [7, 9]. Once internalized, tau seeds encounter (i) endogenous cellular mechanisms aiming to remove misfolded tau or (ii) monomeric tau, which feed the seeding of ingested disease-associated misfolded tau. In addition, and eventually independently from ingested misfolded tau seeds (scenario 2), both oligodendrocytes and astrocytes draw on their cellular MAPT pool for protein production, which can be phosphorylated, misfolded and aggregated into fibrillar, ubiquitinated and argyrophilic coiled bodies and tufted astrocytes, respectively. Pathological tau in oligodendrocytes and astrocytes can also be released for subsequent uptake by both neurons and other glial cells, further accentuating the disease process. Both scenarios potentially occur at the same time and in the same brain region, but scenario 2 may explain reports describing the presence of oligodendrocytic and astrocytic tau-immunopositive inclusions in the absence of neuronal tau-immunopositive inclusions [1, 36, 37, 39, 40, 44]. This notion is further supported by observations that glial tau pathology shows distinct immunoreactivity patterns of phosphorylated tau isoforms compared to neuronal tau pathology [14, 69]. On the other hand, the possibility that glial cells showing tau pathology in areas lacking neuronal tau pathology ingest misfolded tau from synapses of projecting neurons from another brain region should be also considered [35].
This study shows that MAPT RNA transcripts are increased in oligodendrocytic coiled bodies, which was most pronounced in the basal ganglia, an area affected at the earliest disease stages of PSP [41]. In contrast, neurons and astrocytes contained similar, or in some regions, elevated amounts of MAPT transcripts in cells with and without tau-immunopositive inclusions. This suggests MAPT expression is preserved in these cells with tau-immunopositive inclusions, which is sufficient for the cell to draw upon to preserve normal physiological functioning. Furthermore, this data indicates that accumulation of tau and tau-immunopositive inclusions have no negative feedback effect on MAPT expression. However, at some point during the maturation of protein aggregation process for neurons, the neuron will eventually degenerate, as shown by extracellular tangles. Whether a similar degenerative process occurs for tufted astrocytes or whether astrocytes and their physiological machinery are better able to handle fibrillar tau inclusions is yet to be established. The increased amount of MAPT transcripts in oligodendrocytes can be interpreted as follows: (i) the coiled body, which comprises fibrillar and insoluble tau, alters the physiological machinery and functioning of the cell; (ii) the oligodendrocyte recognizes the misfolded tau as abnormal, and in response increases MAPT expression in an attempt to preserve physiological functioning and maintain the production of physiological tau protein. However, the larger cellular pool of MAPT and eventually tau protein that is available can also potentially “feed” the misfolded tau to keep generating/seeding misfolded tau protein and accentuate the pathological process. Alternatively, the increased MAPT expression is not necessarily transcribed into protein. However, one study showed similar trends between MAPT gene and total tau protein expression between brain regions [65]. However, the possibility that accumulation of misfolded tau could have a direct effect on tau transcription in oligodendroglia and this may be impacted by the ability of the cell to degrade tau protein could also be considered. There is currently limited research on how oligodendrocytes are involved in the pathogenesis of PSP and other tau-depositing disorders, even though the diversity of these inclusions is a major feature in the diagnosis and differentiation of these disorders [13]. Oligodendrocytes have important support functions to neurons, and similar to neurons, have an extensive microtubule network indicating that tau in oligodendrocytes might have functions in regulating microtubule-dependent processes. Preclinical models expressing human tau exclusively in oligodendrocytes showed that oligodendrocytic tau accumulation causes disruption of cellular pathways with detrimental effects on their function [15, 25] that eventually lead to a lack of neuronal support and potentially facilitating neurodegeneration.
We observed a dramatic variation in MAPT transcripts between and within cell types even in the same anatomical area, which was also confirmed with snRNAseq showing a wide range of MAPT expression in the frontal cortex of controls and PSP patients. The variation in MAPT expression within and between cells, and between brain regions, has also been reported using total RNA extracted from human [65] and mouse [66] tissue. This variation likely reflects a dynamic process in the human brain, as transcription at the cellular level is constantly changing in response to physiological or pathogenic demands. As expected, MAPT expression was highest in neurons, and results from the snRNAseq study found higher MAPT expression in inhibitory neurons compared to excitatory neurons and also in astrocytes compared to oligodendrocytes. These results are in line with recent MAPT snRNAseq observations on the entorhinal cortex of ageing cynomolgus monkey [43]. As a limitation, our RNAscope study did not differentiate between inhibitory and excitatory neurons, which is likely to account for some of the variation in MAPT expression observed. In addition, since similar MAPT transcript area density in both oligodendrocytes and astrocytes was found with tissue-based RNAscope, which was in contrast to that in snRNAseq, we compared the nuclear area density of MAPT transcripts. This analysis showed higher area density of MAPT transcripts in astrocyte nuclei compared to oligodendrocytes, which aligned with the snRNAseq results. This suggests that oligodendrocytes contain a higher density of MAPT transcripts in their cytoplasm. In addition, snRNAseq showed decreased MAPT expression in inhibitory neurons, OPCs and astroglia in PSP cases compared with controls, indicating that MAPT expression is altered in PSP; we are currently exploring how this contributes to the pathogenesis of disease. However, unlike the RNAscope, the snRNAseq study is not able to evaluate cells with and without tau-immunopositive inclusions. Different methods have been used to evaluate MAPT mRNA expression in human tissue including northern blot, in situ hybridization [22, 23] and RT-PCR using total RNA extracted from human brain tissue [24, 29, 65, 66]. In addition, a website indicating lack of MAPT expression in astrocytes (http://www.brainrnaseq.org) refers to an article [71] using an immunopanning technique and HepaCAM purified astrocytes from human brain tissue obtained from surgeries for treating epilepsy and tumours and using RNAseq. Our study highlights the differences in the MAPT transcription using different methods and shows that the nuclear mRNA expression can be different from cytoplasmic or whole cell mRNA. This has been demonstrated for other genes in the cortex [3] and other tissues [70, 72]. Although our study is not able to demonstrate the level to which the nuclear or cytoplasmic MAPT is transcribed to protein expression, it is interesting to observe nuclear MAPT transcripts in all three cell types in the light of recent studies highlighting the underappreciated role of nuclear tau in the pathogenesis of tauopathies [2, 42]. The strength of our study is that we evaluated a large number of cells and combined the evaluation of protein pathology and MAPT transcripts in the same cells. However, a limitation of this study was that only a small number of control and PSP cases were evaluated and we did not evaluate sex differences in MAPT transcript expression.
A study evaluating the overall brain levels of tau protein and tau mRNA (RT and quantitative PCR) as well as 4R-tau and 3R-tau mRNA levels in the frontal cortex in various main tauopathies revealed decreased levels of tau mRNA with an increase in the 4R tau/3R tau mRNA ratio, but preserved tau protein expression in the frontal cortex in PSP brains [29]. Using a tissue-based approach, we complement the observation of maintained tau protein expression in brain homogenates [29, 65] by showing that accumulation of disease-associated tau protein inclusions is not associated with decreased MAPT transcripts. In accordance with our observations, recent studies confirm that tau accumulation is not a problem at the transcriptional level, because the expression is relatively constant at different points during ageing [66]. Regulation of MAPT expression and splicing is complex [6] and likely different in distinct conditions, and is influenced also by the H1/H2 MAPT haplotype [64, 65]. Our study is not able to address how this process is affected during PSP pathogenesis.
Clinical studies and trials are focusing on therapies targeting tau for PSP and two main strategies have been proposed, which are focused on the elimination of pathological tau aggregates or reducing MAPT expression with ASO therapies to reduce the amount of tau protein transcribed to prevent its aggregation [5]. However, whether these anti-tau strategies need further consideration is still debated [12, 27]. Our study indicates that the preserved MAPT expression in cells accumulating tau protein inclusions might lead to the feeding of the seeding of misfolded tau by constant production of physiological tau. Therefore, a dual-hit strategy could be considered combining the reduction of MAPT transcription together with targeting the elimination of misfolded tau. Furthermore, since tau cytopathology does not compromise MAPT expression in PSP, and supported by studies on an RNA and protein level using brain homogenates [29, 65, 66], a profound reduction or complete loss of tau protein expression (proteopenia) as a pathogenic component is less likely, while continuous feeding of cellular tau seeding by physiological tau administered for therapeutic reasons might even accentuate the pathological process.
Conclusions
In PSP and other tau-related neurodegenerative diseases, there is active debate as to whether there is uptake and internalization of tau by glial cells, or whether glia can build fibrillar tau inclusions independently, without an external source. How and why tau accumulates in oligodendrocytes and astrocytes in PSP is not entirely clear, but our study highlights two potential mechanistic pathways for the cell-to-cell transmission and accumulation of misfolded tau protein. Confirming the presence of MAPT expression in astrocytes and oligodendrocytes is de facto providing the foundation for tau spreading processes where the uptake seed requires the presence of cell-endogenous tau to be templated into disease specific aggregates [60]. Our observations fine-tune our knowledge on cellular MAPT expression by suggesting that nuclear and cytoplasmic RNA expression of MAPT might be distinct and opens new avenues for the interpretation of the function of MAPT. Importantly, our study provides a rationale for a dual-hit approach to therapies targeting tau aggregation and reduced MAPT transcription. Finally, we highlight novel and previously unexplained aspects of disease pathogenesis that will be relevant for therapy development, basic researchers working on cellular mechanisms of tau-related diseases and opens new avenues for neuropathologists working with human tissue.
Data availability
The annonymised datasets generated during and/or analysed during the current study will be made available from the corresponding author on reasonable request from any qualified investigator.
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Acknowledgements
This study was supported by the Rossy Foundation and Edmond J. Safra philanthropic fund, Canada Foundation for Innovation (CFI; Project Nr. 40480), Sandooq Al Watan grant (AWARD-F2018-002). SLF is supported by the National Health and Medical Research Council of Australia Ideas grant (#214090508). Research reported in this publication was supported by the National Institute on Aging of the National Institutes of Health under Award Number R01AG080001. The authors wish to thank Dr Helen Chasiotis, Nikon Canada Inc., for her excellent technical support.
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SLF: concept and design of study, acquisition and analysis, interpretation of data, drafted the manuscript, accountable for all aspects of the work in ensuring that questions related to the accuracy or integrity of any part of the work are appropriately investigated and resolved. SL: acquisition and analysis of data, critical revision for important intellectual input. NN: acquisition and analysis, interpretation of data, critical revision for important intellectual input. IMV: acquisition of data, critical revision for important intellectual input. VS: acquisition of data, critical revision for important intellectual input. JLL: acquisition of data, critical revision for important intellectual input. AA: acquisition and analysis of data, critical revision for important intellectual input. CT: acquisition of data, critical revision for important intellectual input. LMI: interpretation of data, critical revision for important intellectual input. AEL: interpretation of data, critical revision for important intellectual input. MU: acquisition and analysis, interpretation of data, critical revision for important intellectual input. GGK: concept and design of study, acquisition and analysis, interpretation of data, accountable for all aspects of the work in ensuring that questions related to the accuracy or integrity of any part of the work are appropriately investigated and resolved. All authors revised the manuscript critically for important intellectual content and approved the version to be published.
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AEL has served as an advisor for AbbVie, AFFiRis, Alector, Amylyx, Aprinoia, Biogen, BioAdvance, BlueRock, Biovie, BMS, CoA Therapeutics, Denali, Janssen, Jazz, Lilly, Novartis, Paladin, Pharma 2B, PsychoGenetics, Retrophin, Roche, Sun Pharma, and UCB; received honoraria from Sun Pharma, AbbVie and Sunovion; received grants from Brain Canada, Canadian Institutes of Health Research, Edmond J Safra Philanthropic Foundation, Michael J. Fox Foundation, the Ontario Brain Institute, Parkinson Foundation, Parkinson Canada, and W. Garfield Weston Foundation; is serving as an expert witness in litigation related to paraquat and Parkinson’s disease, received publishing royalties from Elsevier, Saunders, Wiley-Blackwell, Johns Hopkins Press, and Cambridge University Press. GGK is member of the Acta Neuropathologica Editorial Board but was not involved in the editorial handling of this article. GGK has served as an advisor for Biogen; received royalty for 5G4 synuclein antibody and publishing royalties from Wiley, Cambridge University Press and Elsevier, received grants from Edmond J Safra Philanthropic Foundation, Rossy Foundation, Michael J. Fox Foundation, Parkinson Canada, Canada, Canada Foundation for Innovation, MSA Coalition, and National Institutes of Health (NIH). SLF, SL, NN, IMV, VS, JL, AA, MCT, LI, MU have no declarations of interest.
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Forrest, S.L., Lee, S., Nassir, N. et al. Cell-specific MAPT gene expression is preserved in neuronal and glial tau cytopathologies in progressive supranuclear palsy. Acta Neuropathol 146, 395–414 (2023). https://doi.org/10.1007/s00401-023-02604-x
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DOI: https://doi.org/10.1007/s00401-023-02604-x