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Entwicklung einer Biohybridlunge

Dauerhaftes „lung assist device“ oder sogar zukünftige Alternative zur Lungentransplantation?

Development of a biohybrid lung

Durable“lung assist device” or even a future alternative to lung transplantation?

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Zeitschrift für Herz-,Thorax- und Gefäßchirurgie Aims and scope

Zusammenfassung

Die Lungentransplantation stellt die einzige Therapieoption für Patienten mit finalen Lungenerkrankungen dar, da es derzeit kein Lungenunterstützungssystem gibt, das eine ausreichende Kohlendioxidelimination und Sauerstoffoxygenation dauerhaft sicherstellen kann. Eine Transplantation ist jedoch nur einem äußerst selektionierten Patientenkollektiv vorbehalten. Dies ist nicht zuletzt auf die steigende Anzahl von Organempfängern gegenüber einer nahezu konstanten Zahl potenzieller Organspender zurückzuführen. Ziel dieses Projekts ist die Entwicklung einer bioartifiziellen Lunge, die den Gasaustausch beständig gewährleisten soll. Hierzu wird die Oberflächenbeschaffenheit von Poly-4-methyl-1-pentene(PMP)-Gasaustauschmembranen für eine effektive Endothelialisierung modifiziert, die Endothelzellgewinnung aus Progenitorzellen, sowie die Zellbesiedlung von PMP etabliert. Verschiedene Funktionstests dieser endothelialisierten PMP werden durchgeführt, die sich u. a. mit Fragen von Biokompatibilität, Gasaustausch und Zelleffekten durch den PMP-Kontakt beschäftigen. Zusammengefasst ist dieses Projekt ein erster Schritt auf dem Weg zur Entwicklung einer Biohybridlunge.

Abstract

Lung transplantation is the only therapy option for patients with end-stage pulmonary disease, as there is no durable “lung assist device” to replace the main function of the lungs, which is CO2 removal and blood oxygenation. However, transplantation is reserved for a few chosen patients. Unfortunately, the number of patients on the organ transplantation waiting lists is increasing, whereby the number of potential organ donors has remained relatively constant. The goal of this project is the development of a biohybrid lung that provides sufficient long-term gas exchange. The construction includes the optimization of cell seeding onto poly-4-methyl-1-pentene (PMP) gas exchange membranes. This involves the modification of the membrane surface for effective long-term endothelial cell (EC) seeding, EC derivatization from endothelial progenitor cells obtained from human cord blood, EC seeding onto the membranes and various functional testing of these endothelialized membranes, like the effects of PMP contact on the cells, the biocompatibility, and the gas exchange properties. In conclusion, this project represents the first step towards a tissue engineered lung.

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Authors and Affiliations

  1. Klinik für Herz-, Thorax-, Transplantations- und Gefäßchirurgie, Medizinische Hochschule Hannover, Carl-Neuberg-Str. 1, 30625, Hannover, Deutschland

    B. Wiegmann, A. Haverich & S. Fischer

  2. Leibniz Forschungslaboratorien für Biotechnologie und künstliche Organe, Medizinische Hochschule Hannover, Hannover, Deutschland

    C. Hess

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  1. B. Wiegmann
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  2. C. Hess
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  4. S. Fischer
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Correspondence to B. Wiegmann.

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Wiegmann, B., Hess, C., Haverich, A. et al. Entwicklung einer Biohybridlunge. Z Herz- Thorax- Gefäßchir 23, 293–297 (2009). https://doi.org/10.1007/s00398-009-0739-4

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  • DOI: https://doi.org/10.1007/s00398-009-0739-4

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