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Holographic microrheology of polysaccharides from Streptococcus mutans biofilms

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Abstract

We use three-dimensional holographic particle tracking to perform microrheological measurements of model gelled media, including the polysaccharide pellicle of dental biofilms created by the common cariogenic oral pathogen Streptococcus mutans. Nanometer-resolution video-rate holographic tracking of embedded colloidal spheres provides accurate measurements of the gels’ complex viscoelastic moduli, including insights into these properties’ heterogeneity. When applied to polysaccharides of S. mutans biofilms, these techniques promise quantitative microscopic assays for candidate therapeutic agents against cariogenic dental biofilms.

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Acknowledgement

This work was supported by the National Science Foundation under Grant Number DMR-0606415.

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Authors and Affiliations

  1. Department of Physics and Center for Soft Matter Research, New York University, New York, NY, 10003, USA

    Fook Chiong Cheong & David G. Grier

  2. Department of Basic Science and Craniofacial Biology, College of Dentistry, New York University, New York, NY, 10010, USA

    Simone Duarte

  3. Department of Physics, University of California, Berkeley, CA, 94720-7300, USA

    Sang-Hyuk Lee

Authors
  1. Fook Chiong Cheong
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  2. Simone Duarte
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  3. Sang-Hyuk Lee
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  4. David G. Grier
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Correspondence to David G. Grier.

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Cheong, F.C., Duarte, S., Lee, SH. et al. Holographic microrheology of polysaccharides from Streptococcus mutans biofilms. Rheol Acta 48, 109–115 (2009). https://doi.org/10.1007/s00397-008-0320-1

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  • DOI: https://doi.org/10.1007/s00397-008-0320-1

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