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Synthesis and characterization of poly (N-isopropylacrylamide)-g-carboxymethyl chitosan copolymer-based doxorubicin-loaded polymeric nanoparticles for thermoresponsive drug release

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Abstract

Thermoresponsive graft copolymer (PNIPAm-g-OCMC) with PNIPAm and chitosan was prepared by conjugation of amine-terminated PNIPAm with O-carboxymethylchitosan through graft-onto method. The results of FTIR and proton NMR confirmed the synthesis of a copolymer. Furthermore, XRD analysis revealed conjugation reaction of polymeric materials. Doxorubicin-loaded polymeric nanoparticles (D-PNPs) were fabricated using copolymer by gelation method. Morphology illustration through TEM showed the spherical shape of D-PNPs. Particles size measurement study indicates thermoresponsive properties of D-PNPs at various temperatures. Lower critical solution temperature (LCST) of D-PNPs was obtained at 38 °C predicted by turbidimetry analysis. Release studies at three different temperatures notified that thermoresponsive doxorubicin release of D-PNPs. The release of doxorubicin was slower at 25 °C, moderately released at 37 °C, and faster released at 42 °C due to adjustment of the framework of the D-PNPs. Based on the results such as transmittance measurement, particle size observation, and release studies, we declare that the D-PNPs exhibit temperature-responsive behavior, which could make them appropriate for a thermoresponsive drug delivery.

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Acknowledgments

M.Gover Antoniraj is supported by the Maulana Azad National Fellowship for Minority Students sponsored by the University Grants Commission (UGC), Government. of India. We also wish to acknowledge the National Facility for Drug Development funded by the Department of Science and Technology (DST) by the Ministry of Science and Technology of India for their equipment facility in this work.

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Authors and Affiliations

  1. Centre for Excellence in Nanobio Translational Research (CENTRE), Department of Pharmaceutical Technology, Anna University, BIT campus, Tiruchirappalli, 620024, Tamilnadu, India

    M. Gover Antoniraj, C. Senthil Kumar & Ruckmani Kandasamy

  2. National Facility for Drug Development for cademia, Pharmaceutical and Allied Industries (NFDD), Anna University, BIT campus, Tiruchirappalli, 620024, Tamilnadu, India

    Ruckmani Kandasamy

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  1. M. Gover Antoniraj
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  2. C. Senthil Kumar
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  3. Ruckmani Kandasamy
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Correspondence to Ruckmani Kandasamy.

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Antoniraj, M.G., Kumar, C.S. & Kandasamy, R. Synthesis and characterization of poly (N-isopropylacrylamide)-g-carboxymethyl chitosan copolymer-based doxorubicin-loaded polymeric nanoparticles for thermoresponsive drug release. Colloid Polym Sci 294, 527–535 (2016). https://doi.org/10.1007/s00396-015-3804-4

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  • DOI: https://doi.org/10.1007/s00396-015-3804-4

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