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Composite polymer particles with stimuli-responsive surface properties and specific activity of adsorbed/released trypsin

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Abstract

Micron-sized monodispersed stimuli-responsive polys’ (PS)/poly(2-dimethylaminoethyl methacrylate-N-isopropyl acrylamide-ethylene glycol dimethacrylate) [P(DM-NIPAM-EGDM)] composite polymer particles were prepared by seeded copolymerisation of DM, NIPAM and EGDM with PS seed particles. Adsorption behaviour of trypsin suggested that composite particles surface has both temperature- and pH-responsive swelling–deswelling characteristics. The performance of composite polymer particles as a carrier for biomolecules in adsorption/release experiments was evaluated by measuring the specific activities of adsorbed trypsin as a function of temperature and pH.

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Acknowledgment

The authors would like to give thanks to MOSICT, Dhaka, for financial support. Thanks are also due for Professor M. Okubo of Kobe University, Japan for supporting with electron micrographs.

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Authors and Affiliations

  1. Department of Chemistry, Rajshahi University, Rajshahi, 6205, Bangladesh

    M. Ashraful Alam, M. A. Jalil Miah & Hasan Ahmad

Authors
  1. M. Ashraful Alam
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  2. M. A. Jalil Miah
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  3. Hasan Ahmad
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Correspondence to Hasan Ahmad.

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Alam, M.A., Miah, M.A.J. & Ahmad, H. Composite polymer particles with stimuli-responsive surface properties and specific activity of adsorbed/released trypsin. Colloid Polym Sci 285, 715–720 (2007). https://doi.org/10.1007/s00396-006-1638-9

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  • DOI: https://doi.org/10.1007/s00396-006-1638-9

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