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Interactions of hemoglobin with lecithin liposomes

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Abstract

In this paper, the interaction of hemoglobin (Hb) with lecithin liposomes is studied by UV-vis spectroscopy, fluorescent spectroscopy, and transmission electron microscopy. The adsorption of Hb on liposomes is likely to be related to the hydrophobic interaction between Hb and liposomes, which brings about the increase of the microenvironmental polarity (I 1/I 3) and the decrease of the fluorescence polarization (P) of lecithin liposomes. These results are considered to be that the adsorption of Hb on liposomes makes the spaces between the lecithin molecules increase, and a temporary gap is consequently formed in the liposomal bilayer membranes. The leakage of aqueous-space marker from the liposomes is increased with the addition of Hb.

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Acknowledgement

This work was supported by the National Natural Scientific Foundation of China (No. 20233010) and the Natural Scientific Foundation of Jiangsu Provincial Department (02KJB520007).

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Authors and Affiliations

  1. School of Chemistry and Chemical Engineering, Yangzhou University, Yangzhou, 225002, People’s Republic of China

    Juqun Xi, Rong Guo & Xia Guo

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  1. Juqun Xi
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  2. Rong Guo
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  3. Xia Guo
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Correspondence to Rong Guo.

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Xi, J., Guo, R. & Guo, X. Interactions of hemoglobin with lecithin liposomes. Colloid Polym Sci 284, 1139–1145 (2006). https://doi.org/10.1007/s00396-006-1492-9

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  • DOI: https://doi.org/10.1007/s00396-006-1492-9

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