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Preparation, characterization and application of pyrene-loaded methoxy poly(ethylene glycol)–poly(lactic acid) copolymer nanoparticles

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Abstract

Pyrene-loaded biodegradable polymer nanoparticles were prepared by incorporating pyrene into the polymer nanoparticles formulated from amphiphilic diblock copolymer, methoxy poly(ethylene glycol)–poly(lactic acid) (MePEG–PLA). Their morphological structure and physical properties were characterized by nuclear magnetic resonance (NMR), dynamic light scattering, fluorescence spectroscopy, transmission electronic microscopy and zeta potential measurements. Further, MePEG–PLA nanoparticles containing pyrene as fluorescent marker were administered intranasally to rats, and the distribution of nanoparticles in the nasal mucosa and the olfactory bulb were visualized by fluorescence microscopy. NMR results confirmed that MePEG–PLA copolymer can form nanoparticles in water, and hydrophilic PEG chains were located on the surface of the nanoparticles. The particle size, zeta potential and pyrene loading efficiency of MePEG–PLA nanoparticles were dependent on the PLA block content in the copolymer. Following nasal administration, the absorption of nanoparticles across the epithelium was rapid, with fluorescence observed in the olfactory bulb at 5 min, and a higher level of fluorescence persisted in the olfactory mucosa than that in the respiratory mucosa. These results show that pyrene could serve as a useful fluorescence probe for incorporation into polymer nanoparticles to study tissue distribution and MePEG–PLA nanoparticles might have a great potential as carriers of hydrophobic drugs.

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Acknowledgements

The author is grateful for the financial support of Med-X Foundation of Fudan University and “863 Project” (No.2001AA218011).

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Authors and Affiliations

  1. Department of Macromolecular Science of Fudan University and the Key Laboratory of Molecular Engineering of Polymers, Ministry of Education, 200433, Shanghai, China

    Yan Zhang, Wuli Yang, Changchun Wang & Shoukuan Fu

  2. School of Pharmacy, Fudan University, 200032, Shanghai, China

    Qizhi Zhang & Xinguo Jiang

  3. College of Material Science and Technology, Donghua University, 200051, Shanghai, China

    Liusheng Zha

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  1. Yan Zhang
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  2. Qizhi Zhang
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  3. Liusheng Zha
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  4. Wuli Yang
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  7. Shoukuan Fu
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Correspondence to Shoukuan Fu.

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Zhang, Y., Zhang, Q., Zha, L. et al. Preparation, characterization and application of pyrene-loaded methoxy poly(ethylene glycol)–poly(lactic acid) copolymer nanoparticles. Colloid Polym Sci 282, 1323–1328 (2004). https://doi.org/10.1007/s00396-004-1068-5

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  • DOI: https://doi.org/10.1007/s00396-004-1068-5

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