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Ischemia/reperfusion injury is increased and cardioprotection by a postconditioning protocol is lost as cardiac hypertrophy develops in nandrolone treated rats

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Abstract

We hypothesized that nandrolone (ND)-abuse induces cardiac hypertrophy, increases myocardial susceptibility to ischemia/reperfusion (I/R) injury, and reduces responsiveness to postconditioning (PostC) cardioprotection. Wistar-rats were ND treated for 2 weeks (short_ND) or 10 weeks (long_ND). Vehicle-treated rats served as controls. Hearts were retrogradely perfused and left ventricular pressure (LVP) was measured before and after 30-min global ischemia. In subgroups of hearts, to induce cardioprotection a PostC protocol (five cycles of 10-s reperfusion and 10-s ischemia) was performed. β-adrenoreceptors, kinases (Akt and GSK-3β) and phosphatases (PP2A sub A and PP2A sub B) were examined by Western blot before and after ischemia. After 120-min reperfusion, infarct size was measured. Short_ND slightly increased cardiac/body weight ratio, but did not affect cardiac baseline nor post-ischemic contractile function or infarct size when compared to vehicle hearts. However, PostC limited cardiac dysfunction much more in short_ND hearts than the other groups. Although cardiac/body weight ratio markedly increased after long_ND, baseline LVP was not affected. Yet, post-ischemic contracture and infarct size were exacerbated and PostC was unable to reduce infarct size and ventricular dysfunction. While short_ND increased phosphatases, non-phosphorylated and phosphorylated Akt, long_ND reduced phosphatase-expression and Akt phosphorylation. Both short_ND and long_ND had no effect on the GSK-3β-phosphorylation but increased the expression of β2-adrenoreceptors. In reperfusion, PostC increased Akt phosphorylation regardless of protective effects, but reduced phosphatase-expression in protected hearts only. In conclusion, short_ND improves post-ischemic myocardial performance in postconditioned hearts. However, long_ND increases myocardial susceptibility to I/R injury and abolishes cardioprotection by PostC. This increased susceptibility might be related to steroid-induced hypertrophy and/or to altered enzyme expression/phosphorylation.

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Acknowledgments

The authors were supported by Compagnia di S. Paolo, National Institutes of Cardiovascular Research (INRC, FM, PP); Regione Piemonte (CP, PP, SR), PRIN (CP, PP), ex-60% (CP, PP, SR). We would thank Prof. D. Gattullo and Dr D. Mancardi for their invaluable support and help.

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  1. Department of Clinical and Biological Sciences, “S. Luigi Gonzaga” Hospital, University of Turin, Regione Gonzole 10, 10043, Orbassano (TO), Italy

    C. Penna, F. Tullio, M.-G. Perrelli, F. Moro, G. Abbadessa, F. Piccione, V. Carriero, S. Racca & P. Pagliaro

  2. Italian Institute for Cardiovascular Research (INRC), Via Irnerio 48, 40126, Bologna, Italy

    C. Penna, F. Moro & P. Pagliaro

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Penna, C., Tullio, F., Perrelli, MG. et al. Ischemia/reperfusion injury is increased and cardioprotection by a postconditioning protocol is lost as cardiac hypertrophy develops in nandrolone treated rats. Basic Res Cardiol 106, 409–420 (2011). https://doi.org/10.1007/s00395-010-0143-y

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