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Adipocyte-derived lipids increase angiotensin-converting enzyme (ACE) expression and modulate macrophage phenotype

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Abstract

Human monocytes/macrophages express the angiotensin-converting enzyme (ACE) but nothing is known about its role under physiological conditions. As adipose tissue contains resident macrophages that have been implicated in the generation of insulin resistance in expanding fat mass, we determined whether adipocytes release factors that affect ACE expression and function in monocytes. Incubation of human monocyte-derived macrophages with conditioned medium from freshly isolated human adipocytes (BMI = 25.4 ± 0.96) resulted in a 4-fold increase in ACE expression. The effect was insensitive to denaturation and different proteases but abolished after lipid extraction. mRNA levels of the major histocompatibility complex class II protein increased in parallel with ACE, whereas the expression of tumor necrosis factor-α (TNF-α), monocyte chemoattractant protein-1 (MCP-1), interleukin (IL)-6, and cyclooxygenase-2 decreased. As a consequence of the reduction in MCP-1, monocyte recruitment was also attenuated. Moreover, adipocyte-conditioned medium prevented the interferon (IFN)-γ induced formation of TNF-α, IL-6, and MCP-1, all markers of classically-activated (M1 type) macrophages. The decrease in cytokine expression in adipocyte-conditioned medium-treated macrophages was sensitive to ACE silencing by small interfering RNA (siRNA). Accordingly, ACE overexpression in THP-1 cells mimicked the effect of adipocyte-conditioned medium. In both cell types, ACE inhibition failed to affect the changes induced by adipocyte conditioned-medium treatment and ACE overexpression. Thus, the modulation of macrophage polarization by ACE appears to be mediated independently of enzyme activity, probably via intracellular signaling. Interestingly, human macrophage ACE expression was also upregulated by IL-4 and IL-13, which promote the “alternative” activation of macrophages and decreased by LPS and IFN-γ. Mechanistically, adipocyte-conditioned medium stimulated the phosphorylation of the AMP-activated protein kinase and AMPK inhibition prevented the increase in ACE expression. Moreover, ACE expression was reduced in spleen derived-monocytes from AMPKα1−/− mice versus their wild-type littermates. These data indicate that mature adipocytes modulate the expression profile of macrophages by releasing lipid mediators that increase ACE expression via AMPK. This prevents the pro-inflammatory cytokine production by macrophages.

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Acknowledgments

The authors are indebted to Marie von Reutern and Isabel Winter for expert technical assistance. The experimental work described in this manuscript was supported by the Deutsche Forschungsgemeinschaft (FL 364/2-2, SFB 834/A5 and Exzellenzcluster 147 “Cardio-Pulmonary Systems”).

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Authors and Affiliations

  1. Institute for Vascular Signalling, Goethe-University, Theodor-Stern-Kai 7, 60590, Frankfurt am Main, Germany

    Karin Kohlstedt, Caroline Trouvain & Ingrid Fleming

  2. Institute of Biochemistry I, Goethe-University, Theodor-Stern-Kai 7, 60590, Frankfurt am Main, Germany

    Dmitry Namgaladze

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  1. Karin Kohlstedt
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  2. Caroline Trouvain
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Correspondence to Karin Kohlstedt.

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Kohlstedt, K., Trouvain, C., Namgaladze, D. et al. Adipocyte-derived lipids increase angiotensin-converting enzyme (ACE) expression and modulate macrophage phenotype. Basic Res Cardiol 106, 205–215 (2011). https://doi.org/10.1007/s00395-010-0137-9

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