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Bifidobacterium Lactis Probio-M8 regulates gut microbiota to alleviate Alzheimer’s disease in the APP/PS1 mouse model

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Abstract

Purpose

Studies have shown that Alzheimer’s disease is associated with significant alterations in the gut microbiota. But the effect of probiotics and/or prebiotics on Alzheimer’s disease still remains to be explored. The aim of this study was to determine whether Bifidobacterium Lactis Probio-M8 could alleviate Alzheimer’s disease pathophysiologies in the APP/PS1 transgenic mouse model.

Methods

4-month old APP/PS1 mice were randomly put into two groups and fed with either Probio-M8 or saline water for 45 days. Fecal samples of mice were collected at the beginning and the end of the treatment period to determine the composition of the gut microbiota via 16S ribosomal RNA sequencing technology. The number and size of Aβ plaques in the brain were quantified. In addition, Y maze, novel object recognition and nest building were employed to access cognitive function in the 8-months old APP/PS1 mice at the end of the treatment period.

Conclusion

Our results demonstrated that Probio-M8 reduced Aβ plaque burden in the whole brain and protected against gut microbiota dysbiosis. Furthermore, Probio-M8 could alleviate cognitive impairment in the APP/PS1 mouse.

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Acknowledgements

The authors would like to thank Heping Zhang, Zhihong Sun and all the staff of the Key Laboratory of Dairy Biotechnology and Engineering for critical comments. We thank the Beijing Scitop Bio-tech Co Ltd for providing Probio-M8 strain. We also thank Maojin Yao for his insightful suggestions. We thank Wenliang Lei for his support with experimental apparatus.

Funding

This research was supported by the National Key R&D Program of China (2018YFD0901101); General Program of Natural Science Foundation of Guangdong Province of China (2019A1515012230); Key Research and Development Project of Guangdong Province of China (2019B020210002); Fundamental Research Funds for the Central Universities (2019KZ01); National Natural Science Foundation of China (31671804).

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  1. Jiaoyan Ren

    Present address: School of Food Science and Engineering, South China University of Technology, 381 Wu Shan Road, Guangzhou, 510641, China

Authors and Affiliations

  1. School of Food Science and Engineering, South China University of Technology, 381 Wu Shan Road, Guangzhou, 510641, China

    Jianing Cao, William Kwame Amakye & Chunli Qi

  2. Beijing Scitop Bio-Tech Co. Ltd., Beijing, China

    Xiaojun Liu & Jie Ma

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Contributions

JC: investigation, formal analysis, visualization, writing—original draft, writing—review and editing. WKA: writing—original draft, writing—review and editing. CQ: methodology, investigation, visualization. XL: investigation, resources. jm: resources, methodology. JR: conceptualization, funding acquisition, supervision.

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Correspondence to Jiaoyan Ren.

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Cao, J., Amakye, W.K., Qi, C. et al. Bifidobacterium Lactis Probio-M8 regulates gut microbiota to alleviate Alzheimer’s disease in the APP/PS1 mouse model. Eur J Nutr 60, 3757–3769 (2021). https://doi.org/10.1007/s00394-021-02543-x

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  • DOI: https://doi.org/10.1007/s00394-021-02543-x

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