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The immunomodulatory properties of viable Lactobacillus salivarius ssp. salivarius CECT5713 are not restricted to the large intestine

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Abstract

Purpose

The aim of this study was to better characterise the biological effects of Lactobacillus salivarius ssp. salivarius CECT5713, a probiotic with immunomodulatory properties.

Methods

Live or dead probiotic was assayed in the TNBS model of rat colitis to determine whether viability was a requisite to exert the beneficial effects. In vitro studies were also performed in Caco-2 cells to evaluate its effects on epithelial cell recovery and IL-8 production. Finally, the probiotic was assayed in the LPS model of septic shock in mice to establish its effects when there is an altered systemic immune response.

Results

The viability of the probiotic was required for its anti-inflammatory activity. The probiotic inhibited IL-8 production in stimulated Caco-2 cells and facilitated the recovery of damaged intestinal epithelium. In LPS-treated mice, the probiotic inhibited the production of TNFα in plasma and lungs and increased the hepatic glutathione content. These effects were associated with an improvement in the altered production of the T-cell cytokines in splenocytes, by reducing IL-2 and IL-5 and by increasing IL-10. Finally, it reduced the increased plasma IgG production in LPS-treated mice.

Conclusion

The anti-inflammatory effects of viable L. salivarius ssp. salivarius CECT5713 are not restricted to the gastrointestinal tract.

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Acknowledgments

This study was supported by the Spanish Ministry of Science and Innovation (SAF2008-02616) with funds from the European Union and by Junta de Andalucia (CTS 164). Mònica Comalada is a recipient of Ramon y Cajal Programme from Spanish Ministry of Science and Technology. Desirèe Camuesco is a postdoctoral fellow of the Spanish Ministry of Science and Innovation; Maria Elena Rodríguez-Cabezas is a postdoctoral fellow of CIBER-EHD. The CIBER-EHD is funded by the Instituto de Salud Carlos III.

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Authors and Affiliations

  1. Centro de Investigaciones Biomédicas en Red, Enfermedades Hepáticas y Digestivas (CIBER-EHD), Department of Pharmacology, Center for Biomedical Research, University of Granada, Avenida del Conocimiento s/n, 18100, Armilla, Granada, Spain

    Belén Arribas, Natividad Garrido-Mesa, Laura Perán, Elvira Bailón, Jordi Xaus, Antonio Zarzuelo, Maria Elena Rodríguez-Cabezas & Julio Gálvez

  2. Institute of Cell and Molecular Science Queen Mary College, University of London, London, UK

    Desirée Camuesco, Laurens Kruidenier & Ian R. Sanderson

  3. Institute of Research in Biomedicine (IRB), Macrophage Biology Group, Barcelona, Spain

    Mònica Comalada

  4. Department of Immunology and Animal Sciences, Biosearch Life, Granada, Spain

    Mónica Olivares

Authors
  1. Belén Arribas
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  2. Natividad Garrido-Mesa
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  3. Laura Perán
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  4. Desirée Camuesco
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  5. Mònica Comalada
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  6. Elvira Bailón
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  7. Mónica Olivares
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  8. Jordi Xaus
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  9. Laurens Kruidenier
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  10. Ian R. Sanderson
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  11. Antonio Zarzuelo
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  12. Maria Elena Rodríguez-Cabezas
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  13. Julio Gálvez
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Corresponding author

Correspondence to Julio Gálvez.

Additional information

E. Rodríguez-Cabezas and J. Gálvez contributed equally to the supervision of the study.

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Arribas, B., Garrido-Mesa, N., Perán, L. et al. The immunomodulatory properties of viable Lactobacillus salivarius ssp. salivarius CECT5713 are not restricted to the large intestine. Eur J Nutr 51, 365–374 (2012). https://doi.org/10.1007/s00394-011-0221-4

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  • DOI: https://doi.org/10.1007/s00394-011-0221-4

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