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Dietary deoxynucleic acid induces type 2 T-helper immune response through toll-like receptor 9 in mice

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Abstract

Background

It has been shown that dietary nucleotides modulate immune response. Due to their unique properties in immune responses, nucleotides are used as immunonutrition in the field of clinical nutrition.

Aim of the study

In this study, we examined the effect of dietary deoxynucleic acid (DNA) on antigen (Ag)-specific immune response in ovalbumin (OVA)-immunized BALB/c mice and determined the mechanism using toll-like receptor 9 (TLR9) knock-out (KO) mice.

Methods

BALB/c or TLR9 KO mice were fed control and 1% DNA diets and immunized with OVA. Spleen cells from OVA-immunized mice were stimulated with OVA in vitro, and the contents of IFN-γ and IL-4 in supernatants were measured by an ex vivo system. CD11c+ dendritic cells were purified, and ability of cytokine induction to CD4+ cells was examined.

Results

The level of OVA-specific IL-4 production in the DNA group was significantly higher than that in the control group. In contrast, the level of OVA-specific IFN-γ production in the DNA group was lower than that in the control group. The DNA diet decreased Ag-specific IL-4 production and enhanced Ag-specific IFN-γ production in TLR9 KO mice. CD11c+ DCs from mice fed the DNA diet had a greater ability than CD11c+ DCs from mice fed the control diet to induce the production of IL-4 from DO11.10 CD4+ T cells.

Conclusions

Dietary DNA increases Ag-specific IL-4 production and decreases IFN-γ production through a TLR9-dependent pathway. CD11c+ dendritic cells are target cells in dietary DNA-induced immune regulation.

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Acknowledgments

This work was partly supported by Grant-in-Aid for Scientific Research. We thank Drs. Nemoto H. and Kataoka K. for technical analyses.

Conflict of interest

The authors of this research disclose any potential conflict of interest.

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Authors and Affiliations

  1. Department of Public Health and Applied Nutrition, Institute for Health Biosciences, The University of Tokushima, Tokushima, 770-8503, Japan

    Mariko Nakamoto, Emi Shuto, Toshio Hosaka & Tohru Sakai

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  1. Mariko Nakamoto
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  2. Emi Shuto
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  3. Toshio Hosaka
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  4. Tohru Sakai
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Correspondence to Tohru Sakai.

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Nakamoto, M., Shuto, E., Hosaka, T. et al. Dietary deoxynucleic acid induces type 2 T-helper immune response through toll-like receptor 9 in mice. Eur J Nutr 50, 421–426 (2011). https://doi.org/10.1007/s00394-010-0153-4

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  • DOI: https://doi.org/10.1007/s00394-010-0153-4

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