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Effect of supplementation with B vitamins and antioxidants on levels of asymmetric dimethylarginine (ADMA) and C-reactive protein (CRP): a double-blind, randomised, factorial design, placebo-controlled trial

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Abstract

Purpose

Cardiovascular risk factors such as elevated levels of asymmetric dimethylarginine (ADMA)/C-reactive protein (CRP) and homocysteine are potentially related to essential micronutrients such as certain B vitamins and antioxidant vitamins. The aim of the present study was to investigate whether supplementation with moderate doses of B vitamins and/or antioxidants could alter either ADMA and/or CRP concentrations in middle-aged, apparently healthy men with mildly elevated homocysteine levels.

Methods

A randomised, double-blind, factorial design, intervention study was carried out on 132 men with mildly elevated homocysteine levels, allocated to four groups (a) B vitamins alone—1 mg folic acid, 7.2 mg pyridoxine, 0.02 mg cyanocobalamin daily, (b) antioxidants alone—150 mg ascorbic acid, 67 mg vitamin E, 9 mg β-carotene daily, (c) B vitamins with antioxidant vitamins, or (d) placebo. A total of 101 men completed the study to 8 weeks.

Results

When the percentage of baseline ADMA and CRP was examined at 8 weeks, no statistically significant differences were observed between the four groups (p = 0.21 and p = 0.90, respectively). Similar non-significant results were observed when analysis was stratified based on baseline CRP levels (<1.0 mg/L, p = 0.10; ≥1.0 mg/L, p = 0.64) and smoking status (all p ≥ 0.05).

Conclusions

Supplementation with moderate doses of B vitamins and/or antioxidants did not alter either ADMA or CRP concentrations in these middle-aged, apparently healthy men with mildly elevated homocysteine levels.

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Acknowledgments

We acknowledge the Department for Employment and Learning, Belfast for their grant support. Also the technical assistance of Caroline Mercer, Janet Lightbody, Trevor Linton, Drew McIlveen, Maureen McCourt, Geraldine O’Reilly, Kellie Byrne and Ishbel McMillan. We thank the patients for their cooperation, WR Jenkinson (Shorts Brothers Plc, Belfast) and Sandoz Nutrition Ltd (Berne, Switzerland) who supplied the vitamin preparations.

Conflict of interest

The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. School of Medicine, Dentistry and Biomedical Sciences, Queen’s University, Belfast, UK

    Mark G. O’Doherty, Sarah E. C. M. Gilchrist, Ian S. Young, Michelle C. McKinley, John W. G. Yarnell, Alun Evans & Jayne V. Woodside

  2. Vitamin Research Unit, University of Berne, Berne, Switzerland

    K. Fred Gey

  3. University of Otago, Dunedin, New Zealand

    Paula M. L. Skidmore

  4. Cancer Epidemiology Health Services Research Group, Centre for Public Health, Queens University Belfast, Mulhouse Building, Royal Victoria Hospital, Grosvenor Road, Belfast, BT12 6BJ, UK

    Mark G. O’Doherty

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  1. Mark G. O’Doherty
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O’Doherty, M.G., Gilchrist, S.E.C.M., Young, I.S. et al. Effect of supplementation with B vitamins and antioxidants on levels of asymmetric dimethylarginine (ADMA) and C-reactive protein (CRP): a double-blind, randomised, factorial design, placebo-controlled trial. Eur J Nutr 49, 483–492 (2010). https://doi.org/10.1007/s00394-010-0107-x

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