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Two prolamin peptides from durum wheat preclude celiac disease-specific T cell activation by gluten proteins

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Abstract

Purpose

Celiac disease (CD) is a permanent intolerance to wheat prolamins and related proteins displayed by genetically susceptible individuals. Blocking or modulation of CD-specific T cell response by altered prolamin peptides are currently considered as a potential alternative to the only effective therapy of CD based on a life-long gluten-free diet. Two prolamin peptides, the 9-mer ASRVAPGQQ and the 10-mer GTVGVAPGQQ sequences, were identified by mass spectrometry in the peptic/tryptic digest of prolamins (PTP) from durum wheat (Triticum turgidum ssp. durum) cv. Adamello, and investigated for their ability to preclude the stimulation of CD-specific mucosal T cells by gluten proteins.

Methods

Gluten-specific polyclonal intestinal T cell lines from five CD children (mean age 5 years) were exposed to 50 μg/ml of a deamidated PTP from whole flour of common wheat (T. aestivum) cv. San Pastore, and tested for proliferation and production of interferon-γ (INF-γ) and interleukin 10 (IL-10). The same experiment was performed in the presence of 20 μg/ml of the 9-mer or the 10-mer peptide.

Results

T cells exposed to PTP showed a threefold increase in proliferation and INF-γ production, and a significant (P ≤ 0.05) reduction in IL-10 secretion as compared with control cells incubated with the culture medium. Addition of either the 9-mer or the 10-mer peptide to PTP downregulated T cell proliferation and INF-γ production, and caused a significant (P ≤ 0.05) increase in IL-10 secretion.

Conclusions

The T cell reactivity elicited by PTP is precluded by both the 9-mer and the 10-mer sequence, suggesting that over-expression of these proteolytically stable peptides may result in a wheat flour with reduced toxicity for CD patients.

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Conflict of interest statement

The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. Division of Food Safety and Veterinary Public Health, Istituto Superiore di Sanità, Viale Regina Elena, 299, 00161, Rome, Italy

    Massimo De Vincenzi & Olimpia Vincentini

  2. Department of Pediatrics, Gastroenterology and Liver Unit, University of Rome “La Sapienza”, Rome, Italy

    Giovanni Di Nardo

  3. Department of Infectious Parasitic and Immune Mediated Disease, Istituto Superiore di Sanità, Rome, Italy

    Monica Boirivant

  4. CRA-QCE, Research Unit for Cereal Quality, Rome, Italy

    Laura Gazza & Norberto Pogna

Authors
  1. Massimo De Vincenzi
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  2. Olimpia Vincentini
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  3. Giovanni Di Nardo
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  4. Monica Boirivant
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  5. Laura Gazza
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  6. Norberto Pogna
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Correspondence to Olimpia Vincentini.

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De Vincenzi, M., Vincentini, O., Di Nardo, G. et al. Two prolamin peptides from durum wheat preclude celiac disease-specific T cell activation by gluten proteins. Eur J Nutr 49, 251–255 (2010). https://doi.org/10.1007/s00394-009-0080-4

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  • DOI: https://doi.org/10.1007/s00394-009-0080-4

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