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Long chain saturated fatty acids increase haptoglobin gene expression in C57BL/6J mice adipose tissue and 3T3-L1 cells

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Abstract

Background

Dietary lipids are directly related to the composition of adipose tissue, aetiology of obesity and arousal of obesity-related pathologies, like chronic inflammation states. Haptoglobin is an acute phase protein secreted by the liver and white adipose tissue, and its blood levels vary according to the volume of fat in the body.

Aim of the study

To investigate the effect of diets enriched with large amounts of dietary fats, which differ in their fatty acid composition, on the haptoglobin gene expression by visceral and subcutaneous adipose tissue of mice fed for 2 days or 8 weeks. 3T3-L1 cells were treated with fatty acids that are found in those types of dietary fats.

Methods

Mice were treated acutely (for 2 days) or chronically (for 8 weeks) with diets enriched with soybean oil, fish oil, coconut oil or lard. 3T3-L1 cells were treated with six different fatty acids. Haptoglobin gene expression was quantified by northern blotting.

Results

Both chronic and acute treatment with lard, which is rich in long chain saturated fatty acids, increased the haptoglobin mRNA expression in the retroperitoneal and epidydimal white adipose tissues. Chronic treatment with coconut oil, which is rich in medium chain saturated fatty acids, increased the haptoglobin expression in the epidydimal and subcutaneous depots. In 3T3-L1, palmitic acid increased the haptoglobin gene expression.

Conclusion

The type of lipids in the diet can differently modulate the white adipose tissue gene expression of haptoglobin, and saturated fatty acids play a major role in promoting a pro-inflammatory environment. This response is fat pad specific and dependant on the duration of treatment.

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Acknowledgement

The authors thank Dr Luciana Pelegrini Pisani and Mrs Cristiane de Oliveira for technical assistance. This research was supported by CAPES (Coordenação de Aperfeiçoamento de Pessoal de Nível Superior) and FAPESP (Fundação de Amparo à Pesquisa do Estado de São Paulo).

Conflict of interest statement

The authors of this research disclose any potential conflict of interest.

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Authors and Affiliations

  1. Department of Physiology, São Paulo Federal University, Campus São Paulo, Rua Botucatu, 862, Edifício de Ciências Biomédicas, São Paulo, SP, Brazil

    Allain Amador Bueno, Caio Sussumu de Macedo Motoyama, Carolina Rodrigues da Silva Biz, Eliane Beraldi Ribeiro & Cláudia Maria Oller do Nascimento

  2. Institute of Brain Chemistry and Human Nutrition, London Metropolitan University, 166-220 Holloway Road, London, N7 8DB, UK

    Allain Amador Bueno

  3. Department of Biosciences, São Paulo Federal University, Campus Baixada Santista, Av D Ana Costa, 95, Santos, SP, Brazil

    Lila Missae Oyama

  4. Department of Biological Sciences, São Paulo Federal University, Campus Diadema, Rua Botucatu, 862, Edifício de Ciências Biomédicas, São Paulo, SP, Brazil

    Vera Lucia Silveira

  5. Departamento de Fisiologia, Universidade Federal de São Paulo, Rua Botucatu, 862, 2º andar - Edifício de Ciências Biomédicas, São Paulo, SP, 04023060, Brazil

    Cláudia Maria Oller do Nascimento

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  1. Allain Amador Bueno
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Correspondence to Allain Amador Bueno.

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Bueno, A.A., Oyama, L.M., de Macedo Motoyama, C.S. et al. Long chain saturated fatty acids increase haptoglobin gene expression in C57BL/6J mice adipose tissue and 3T3-L1 cells. Eur J Nutr 49, 235–241 (2010). https://doi.org/10.1007/s00394-009-0069-z

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  • DOI: https://doi.org/10.1007/s00394-009-0069-z

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