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Cholecystokinin secretion induced by β-conglycinin peptone depends on Gαq-mediated pathways in enteroendocrine cells

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Abstract

Background

Intraduodenal administration of peptone prepared from soybean β-conglycinin (BconP) stimulates cholecystokinin (CCK) secretion from enteroendocrine cells, and suppresses food intake in rats. However, the sensing mechanism of BconP by CCK-producing cells is unknown.

Aim of the study

We investigated signal transduction pathways mediating CCK secretion in response to BconP in the murine CCK-producing cell line, STC-1.

Methods

STC-1 cells were seeded in 48-well culture plates until sub-confluent and CCK secretion was examined under various conditions. CCK concentration was determined by the enzyme immunoassay.

Results

BconP dose-dependently induced CCK secretion in STC-1 cells. Treatment with BAPTA-AM, an intracellular Ca2+ chelator, reduced BconP-induced CCK secretion, however, removal of extracellular Ca2+ did not affect the secretory response. Treatment with 2-amino borate (2-APB) reduced CCK releasing responses, suggesting the involvement of IP3. In addition, BconP failed to induce CCK secretion after treatment with the Gαq protein inhibitor (YM-254890).

Conclusion

These results indicate that Gαq pathway is responsible for BconP-induced CCK secretion in STC-1 cells, and suggest the involvement of a Gαq-coupled GPCR(s) in dietary peptide sensing in enteroendocrine cells.

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Acknowledgments

This work was funded by Bio-oriented Technology Research Advancement Institution (Japan). We thank Astellas Pharma Inc. for providing YM-254890.

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Authors and Affiliations

  1. Division of Applied Biosciences, Research Faculty of Agriculture, Hokkaido University, Kita-9, Nishi-9, Kita-ku, Sapporo, 060-8589, Japan

    Tohru Hira, Toshihiro Maekawa, Kozo Asano & Hiroshi Hara

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  1. Tohru Hira
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  2. Toshihiro Maekawa
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  3. Kozo Asano
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  4. Hiroshi Hara
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Correspondence to Tohru Hira.

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Hira, T., Maekawa, T., Asano, K. et al. Cholecystokinin secretion induced by β-conglycinin peptone depends on Gαq-mediated pathways in enteroendocrine cells. Eur J Nutr 48, 124–127 (2009). https://doi.org/10.1007/s00394-008-0764-1

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  • DOI: https://doi.org/10.1007/s00394-008-0764-1

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