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Faecal steroid excretion in humans is affected by calcium supplementation and shows gender-specific differences

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Abstract

Background

Previous human studies on the effect of dietary calcium supplementation on faecal excretion of bile acids (BA) and faecal water concentrations of animal neutral sterols (NSt, cholesterol and its metabolites) lack detailed information about single BA and NSt.

Aim of the study

We investigated whether single BA and NSt in faeces and especially in faecal water are affected by calcium supplementation and whether this affects genotoxicity of faecal water. In addition, we differentiated between men and women with regard to the concentrations of BA and NSt in faecal water.

Methods

Thirty-one healthy volunteers consumed a calcium supplemented bread (1.0 g/day) and a placebo bread, respectively, for 4 weeks in a double-blind, randomised cross-over trial. Faeces were collected quantitatively for 5 days in the last week of each period. NSt and BA were analysed by GC–MS.

Results

Due to calcium supplementation faecal concentrations of lithocholic acid (LCA, 14%, P = 0.008), deoxycholic acid (DCA, 19%, P < 0.001) and 12keto-deoxycholic acid (12keto DCA, 29%, P = 0.049) significantly increased whereas BA concentrations in faecal water were only marginally affected. In contrast, concentrations of cholesterol (30%, P = 0.020) and its metabolites coprostanol (43%, P = 0.004), coprostanone (36%, P = 0.003), cholestanol (44%, P = 0.001) and cholestenone (32%, P = 0.038) in faecal water significantly decreased. Total NSt concentration in faecal water was found to be significantly higher in women compared to men (P = 0.018). The genotoxicity of faecal water was neither affected by calcium supplementation nor were there gender-specific differences.

Conclusions

Dietary calcium supplementation diversely affects BA and NSt in faeces and in faecal water but does not influence the genotoxicity of faecal water in healthy adults.

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Authors and Affiliations

  1. Dept. of Nutritional Physiology, Institute of Nutrition, Friedrich Schiller University, Dornburger Straße 24, 07743, Jena, Germany

    Bianka Ditscheid, Sylvia Keller & Gerhard Jahreis

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  1. Bianka Ditscheid
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  2. Sylvia Keller
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  3. Gerhard Jahreis
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Correspondence to Gerhard Jahreis.

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Ditscheid, B., Keller, S. & Jahreis, G. Faecal steroid excretion in humans is affected by calcium supplementation and shows gender-specific differences. Eur J Nutr 48, 22–30 (2009). https://doi.org/10.1007/s00394-008-0755-2

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  • DOI: https://doi.org/10.1007/s00394-008-0755-2

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