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Lyophilized carrot ingestion lowers lipemia and beneficially affects cholesterol metabolism in cholesterol–fed C57BL/6J mice

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Summary

Background

Several lines of evidence indicate that diet rich in fruit and vegetable can protect against cardiovascular diseases by acting on cholesterol metabolism and on oxidative stress.

Aim of the study

The aim of this study was to assess whether daily carrot consumption (provided as lyophilized powder) could differentially influence the consequences of cholesterol supplementation on lipid metabolism and oxidative stress in C57BL/6J mice.

Methods

Fourteen mice were randomized in four groups. Mice were fed either control diets (without or with 0.25% cholesterol added) or lyophilized carrot enriched diets (20% wt/wt without or with 0.25 % cholesterol added) for 4 weeks. Cholesterol and triglycerides in plasma and in liver were measured at the end of the experimental period. Fecal excretion of sterols was evaluated. Vitamin E and carotenoid concentrations were also determined. Several biomarkers relative to oxidative stress such as FRAP (Ferric Reducing Ability of Plasma) and isoprostanes were investigated.

Results

Feeding the carrot diet resulted in a decrease of cholesterol (–41%) and triglycerides (–49 %) in plasma and in the liver (–41% and –39%, respectively) in animals fed cholesterol–supplemented diets. Carrot diet induced an increase of total neutral sterols fecal excretion, which inhibits digestive cholesterol absorption. Carrot diet increased antioxidant status in cholesterolfed mice as related by the 16% higher FRAP values. Although vitamin E was not affected by carrot diet, vitamin E/TG ratio was significantly higher in animals fed carrot diets. The carrot diet induced an increase of vitamin E in the heart in both cholesterol–free and cholesterol–supplemented mice suggesting a higher protection of this tissue.

Conclusion

This study shows that carrot ingestion decreases lipemia and improves antioxidant status in mice. Such results suggest that carrot intake may exert a protective impact against CVD linked to atherosclerosis. It is likely that these effects could be due to the synergistic effect of fiber and associated antioxidants.

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Abbreviations

FRAP:

Ferric Reducing Ability of Plasma

TG:

triacylglycerol

LDL:

low–density lipoprotein

VLDL:

very low–density lipoprotein

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Authors and Affiliations

  1. Vilmorin, Clause & Cie – BP 1, Chappes, France

    C. Nicolle Ph.D & P. Amouroux

  2. Unité des Maladies Métaboliques & Micronutriments, INRA Clermont-Ferrand/Theix, 63122, Saint Genès Champanelle, France

    C. Nicolle Ph.D, E. Gueux, C. Lab, L. Jaffrelo, E. Rock, A. Mazur & C. Rémésy

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  1. C. Nicolle Ph.D
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  2. E. Gueux
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  3. C. Lab
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  4. L. Jaffrelo
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  6. A. Mazur
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Correspondence to C. Nicolle Ph.D.

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Nicolle, C., Gueux, E., Lab, C. et al. Lyophilized carrot ingestion lowers lipemia and beneficially affects cholesterol metabolism in cholesterol–fed C57BL/6J mice. Eur J Nutr 43, 237–245 (2004). https://doi.org/10.1007/s00394-004-0465-3

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  • DOI: https://doi.org/10.1007/s00394-004-0465-3

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