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Differential increase of CD34, KDR/CD34, CD133/CD34 and CD117/CD34 positive cells in peripheral blood of patients with acute myocardial infarction

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Abstract

Objective

Circulating progenitor cells (CPC) may contribute to cardiac regeneration and neovascularization after acute myocardial infarction (AMI). For potential therapeutic use, understanding the endogenous mechanisms after ischemia is inevitable. We investigated the absolute number, but also the subset composition of CD34+ CPC after AMI.

Methods

CD34+, KDR+/ CD34+, CD133+/CD34+ and CD117+/CD34+ CPC were analyzed by FACS in peripheral blood of 10 patients with acute MI (59±5 yrs, m/f=8/2) at day of AMI (day 0) and days 1–5. For comparison patients with stable coronary artery disease (CAD, n=12, 66±2 yrs, m/f=10/2) and young healthy volunteers (n=7, 26±2 yrs, m/f=3/4) were studied.

Results

CD34 and KDR/CD34, CD133/CD34, CD117/CD34 were increased day 3 and 4 after AMI. KDR+ fraction within CD34+ population remained unchanged (58.3±7.8% vs 55.3±10.6%), whereas CD133+ (64.9±3.1% vs 43.5±5.9%, P=0.006) and CD117+ fractions (71.7±5.6% vs 50.1±5.5%, P=0.02) were elevated. In CAD, all CPC and fractions were similar as AMI day 0. Healthy volunteers had more CD34+ than CAD and AMI day 0. Double positive CPC were also higher, but fractions were unchanged vs CAD with more KDR/CD34 in trend (72.8±10.6% vs 50.5±5.6%, P=0.058). After AMI both absolute numbers of CD34+ and their subset composition change, suggesting selective mobilization of CPC. Increased CPC after AMI never reach numbers of young healthy volunteers.

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Authors and Affiliations

  1. Laboratory of Muscle Research and Molecular Cardiology, Department of Internal Medicine III, University of Cologne, Cologne, Germany

    Franziska Grundmann MD, Daniela Braun MS, Carsten Zobel MD, Hannes Reuter MD, Robert H. G. Schwinger MD &  Jochen Müller-Ehmsen MD

  2. Laboratory of Bone Marrow, Transplantation, Department of Internal Medicine I, University of Cologne, Cologne, Germany

    Christof Scheid MD

  3. Department of Internal Medicine III, University of Cologne, Kerpener Str. 62, 50937, Köln/Cologne, Germany

    Jochen Müller-Ehmsen MD

Authors
  1. Franziska Grundmann MD
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  2. Christof Scheid MD
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  3. Daniela Braun MS
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  4. Carsten Zobel MD
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  5. Hannes Reuter MD
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  6. Robert H. G. Schwinger MD
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  7. Jochen Müller-Ehmsen MD
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Correspondence to Jochen Müller-Ehmsen MD.

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Grundmann, F., Scheid, C., Braun, D. et al. Differential increase of CD34, KDR/CD34, CD133/CD34 and CD117/CD34 positive cells in peripheral blood of patients with acute myocardial infarction. Clin Res Cardiol 96, 621–627 (2007). https://doi.org/10.1007/s00392-007-0543-7

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  • DOI: https://doi.org/10.1007/s00392-007-0543-7

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