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NF-κB p65 phosphorylated at serine-536 is an independent prognostic factor in Swedish colorectal cancer patients

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International Journal of Colorectal Disease Aims and scope Submit manuscript

Abstract

Introduction

The NF-κB transcription factor protein family has diverse cellular and biological functions, and posttranslational modification is important to regulate these functions. An important site of phosphorylation of NF-κB p65 subunit is at serine-536 (phospho-Ser536-p65), and this phosphorylation is involved in regulation of transcriptional activity, nuclear localization, and protein stability.

Patients and Methods

In this study, we investigated expression of phospho-Ser536-p65 in colorectal cancers and its relationships with clinicopathological factors. The expression of phospho-Ser536-p65 was examined by immunohistochemistry in 203 primary colorectal cancers, 156 normal mucosa specimens, and 18 metastases in the lymph nodes.

Results

The expression of phospho-Ser536-p65 increased from normal mucosa to primary tumor (p < 0.0001). Further, the increased expression of phospho-Ser536-p65 in the cytoplasm of the primary tumors correlated with worse survival of the patients independently of gender, age, tumor location, stage, and differentiation (p = 0.04; hazard ratio, 1.89; 95% CI 1.03–3.47).

Conclusion

The NF-κB p65 subunit phosphorylated at serine-536 is an independent prognostic factor in colorectal cancer patients.

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Acknowledgments

The authors thank Helen Richard, Cecilia Bergenwald, Gertrud Stridh, Gunnel Lindell, and Kerstin Ingels, Department of Pathology, Linköping Hospital, Sweden, for kindly preparing tissue sections, and Dr. David Hinselwood (a Scottish doctor) at the Division of Oncology, Linköping University, Sweden, for revising this paper.

Conflicts of interest

None declared.

Author information

Authors and Affiliations

  1. Division of Oncology, Department of Clinical and Experimental Medicine, Faculty of Health Sciences, Linköping University, SE-581 85, Linköping, Sweden

    Andreas Lewander, Jinfang Gao & Xiao-Feng Sun

  2. Department of Health and Society, Faculty of Health Sciences, Linköping University, SE-581 85, Linköping, Sweden

    John Carstensen

  3. Department of Surgery, Country Council of Östergötland, Norrköping, Sweden

    Gunnar Arbman

  4. Tumour Biology, School of Life Sciences, University of Skövde, Skövde, Sweden

    Hong Zhang

Authors
  1. Andreas Lewander
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  2. Jinfang Gao
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  3. John Carstensen
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  4. Gunnar Arbman
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  5. Hong Zhang
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  6. Xiao-Feng Sun
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Corresponding author

Correspondence to Xiao-Feng Sun.

Additional information

The study was supported by grants from the Swedish Cancer Foundation, the Swedish Research Council, and the Health Research Council in the Southeast of Sweden.

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Lewander, A., Gao, J., Carstensen, J. et al. NF-κB p65 phosphorylated at serine-536 is an independent prognostic factor in Swedish colorectal cancer patients. Int J Colorectal Dis 27, 447–452 (2012). https://doi.org/10.1007/s00384-011-1356-8

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  • DOI: https://doi.org/10.1007/s00384-011-1356-8

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