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B1a lymphocytes in ulcerative colitis

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International Journal of Colorectal Disease Aims and scope Submit manuscript

Abstract

Background and aims

B1a lymphocytes (CD5+) are the major contributors of natural antibodies (Ab) implicated in the initial protection against several infections. The aim of this study was to assess the expression of these cells in the peripheral blood of ulcerative colitis (UC) patients who underwent restorative proctocolectomy (RPC) and others who were not operated on.

Materials and methods

The blood concentration of CD5+ B cells was analysed by three-colour flow cytometry. Blood was collected from 38 UC patients, 20 of whom had undergone RPC and compared with the results in 18 healthy controls and in 12 familial adenomatous polyposis (FAP) patients who had undergone RPC. We were interested in evaluating if there was any correlation between B1a blood cell concentration and ESR and CRP levels, clinical, endoscopic and histological activity, perinuclear anti-neutrophil cytoplasmic antibody (pANCA) and extra-intestinal symptoms.

Results

B1a cell blood concentration was reduced in non-operated UC patients (20.7 ± 4.6/μl) with respect to that in healthy controls (71.1 ± 18.0/μl, p < 0.05). It was also lower in UC patients with RPC (24.9 ± 1.0/μl) compared to RPC for FAP (48.2 ± 6.2, p < 0.05). B1a cell rate correlated inversely in UC patients with ESR (R = −0.41, p < 0.05) and CRP levels (R = −0.47, p = 0.01).

Conclusion

B1a cell concentration was reduced in the blood of patients with UC even after the diseased organ was surgically removed by proctocolectomy. As these cells play an important role in natural immunity against luminal stimuli, consistently lower levels that are found in UC patients could be responsible for the impaired immunologic response to gut antigens in this disease.

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Acknowledgment

The authors are grateful to Linda Inverso Moretti for her assistance in editing the English version of this manuscript.

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Authors and Affiliations

  1. Department of Surgical and Gastroenterological Sciences, Surgical Unit, University of Padova, Padova, Italy

    Lino Polese, Marco Scarpa, Cesare Ruffolo, Lorenzo Norberto, Mauro Frego, Davide F. D’Amico & Imerio Angriman

  2. Department of Surgical and Gastroenterological Sciences, Gastroenterological Unit, University of Padova, Padova, Italy

    Giacomo C. Sturniolo

  3. Department of Laboratory Medicine, University of Padova, Padova, Italy

    Giuseppe De Franchis

  4. Clinica Chirurgica 1, Policlinico Universitario, Via Giustiniani 2, 35128, Padova, Italy

    Lino Polese

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  1. Lino Polese
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Correspondence to Lino Polese.

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Polese, L., De Franchis, G., Scarpa, M. et al. B1a lymphocytes in ulcerative colitis. Int J Colorectal Dis 22, 1005–1011 (2007). https://doi.org/10.1007/s00384-007-0298-7

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