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Expression of c-kit messenger ribonucleic acid and c-kit protein in sigmoid colon of patients with slow transit constipation

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Abstract

Background and aims

The c-kit protooncogene receptor and its ligand stem cell factor regulate the proliferation and survival of germ cells as well as interstitial cells of Cajal (ICCs). Decreased numbers of ICCs and defects in its networks have been reported in the colon of patients with slow transit constipation (STC). However, little information about the c-kit messenger ribonucleic acid (mRNA) and protein expression in the constipated colon is available. The aim of this study was to determine whether the expression of c-kit mRNA and c-kit protein declined in the colon in STC.

Patients and methods

The sigmoid colonic samples from 12 patients with STC and from eight age-matched patients with non-obstructed colorectal cancer were used for this study. Expression of c-kit mRNA was detected by reverse transcriptase-polymerase chain reaction (RT-PCR), and expression of c-kit protein was detected by Western blot analysis.

Results

Decreased expression of c-kit mRNA was demonstrated in the STC group compared with the control group. The ratio of c-kit and β-actin was 1.26±0.32 in controls and 1.17±0.41 in the STC group (U=0.500, P=0.029). c-kit protein expression significantly declined in the STC group. The mean value of optical density was 162.97±5.43 in the control group and 96.64±8.80 in the STC group (U=0.000, P=0.021).

Conclusions

The data indicate that the expression of c-kit mRNA and c-kit protein significantly decreased in the colon of STC, suggesting that the c-kit signal pathway may play an important role in ICC reduction in STC.

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Acknowledgements

This work was supported by a grant from the National Natural Science Foundation of China (NO. 30300156).

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Authors and Affiliations

  1. Department of General Surgery, Surgery Research Institute, Daping Hospital, Third Military Medical University, Chongqing, 400042, China

    Wei-Dong Tong, Bao-Hua Liu, Lian-Yang Zhang & Sheng-Ben Zhang

  2. Department of Molecular Biology, Surgery Research Institute, Daping Hospital, Third Military Medical University, Chongqing, China

    Ren-Ping Xiong & Ping Liu

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  1. Wei-Dong Tong
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Correspondence to Wei-Dong Tong.

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Tong, WD., Liu, BH., Zhang, LY. et al. Expression of c-kit messenger ribonucleic acid and c-kit protein in sigmoid colon of patients with slow transit constipation. Int J Colorectal Dis 20, 363–367 (2005). https://doi.org/10.1007/s00384-004-0679-0

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