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Association of circulating osteopontin levels with clinical outcomes in postoperative biliary atresia

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Abstract

Purpose

Biliary atresia (BA) is one of the most serious liver disorders in children. The aims of the present study were to investigate circulating levels of osteopontin in BA children compared with healthy controls and to evaluate the relationship between circulating osteopontin and therapeutic outcome of BA patients.

Methods

Fifty-nine BA patients post-Kasai operation and 13 healthy children were recruited. The patients were divided into two groups according to their serum total bilirubin levels (TB < 2, jaundice-free vs. TB ≥ 2 mg/dL, persistent jaundice) and alanine aminotransferase (ALT < 45, normal ALT vs. ALT ≥ 45 IU/L, elevated ALT). Plasma osteopontin levels were analyzed using commercial enzyme-linked immunosorbent assay.

Results

The circulating osteopontin was higher in BA children compared with that of healthy controls (146.9 ± 19.1 vs. 28.0 ± 8.4 ng/mL, P = 0.001). The BA patients with persistent jaundice had more increased plasma osteopontin levels than those without jaundice (157.8 ± 47.9 vs. 27.5 ± 6.4 ng/mL, P = 0.001). Furthermore, plasma osteopontin levels in BA patients with elevated ALT were significantly higher than those with normal ALT (103.2 ± 29.2 vs. 24.5 ± 7.9 ng/mL, P = 0.01). In addition, circulating osteopontin was positively correlated with serum total bilirubin (r = 0.526, P < 0.001) and with serum ALT (r = 0.575, P < 0.001). Subsequent analysis showed that the BA patients with portal hypertension had more elevated plasma osteopontin compared to those without portal hypertension (116.7 ± 31.1 vs. 19.5 ± 9.3 ng/mL, P = 0.01).

Conclusion

Increased circulating osteopontin was associated with the development of hepatic dysfunction and portal hypertension in BA patients. Circulating osteopontin may serve as a possible marker reflecting disease severity and monitoring the disease progression in postoperative BA patients.

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Acknowledgments

The present study has been supported by grants from Ratchadapiseksompotch Fund, Faculty of Medicine, Chulalongkorn University, Thailand Research Fund, and the Commission on Higher Education. The authors are profoundly grateful to the entire staff of the Center of Excellence in Clinical Virology, Chulalongkorn University and King Chulalongkorn Memorial Hospital for their combined effort in this study.

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Authors and Affiliations

  1. Department of Biochemistry, Faculty of Medicine, Chulalongkorn University and King Chulalongkorn Memorial Hospital, Rama IV road, Patumwan, Bangkok, 10330, Thailand

    Sittisak Honsawek

  2. Department of Surgery, Faculty of Medicine, Chulalongkorn University, Bangkok, 10330, Thailand

    Paisarn Vejchapipat

  3. Department of Pediatrics, Faculty of Medicine, Center of Excellence in Clinical Virology, Chulalongkorn University, Bangkok, 10330, Thailand

    Voranush Chongsrisawat, Nutchanart Thawornsuk & Yong Poovorawan

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  1. Sittisak Honsawek
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  2. Paisarn Vejchapipat
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  4. Nutchanart Thawornsuk
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  5. Yong Poovorawan
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Correspondence to Sittisak Honsawek.

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Honsawek, S., Vejchapipat, P., Chongsrisawat, V. et al. Association of circulating osteopontin levels with clinical outcomes in postoperative biliary atresia. Pediatr Surg Int 27, 283–288 (2011). https://doi.org/10.1007/s00383-010-2799-7

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  • DOI: https://doi.org/10.1007/s00383-010-2799-7

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