Abstract
Polysialyated neural cell adhesion molecule (PSA-NCAM) is a marker for immature neurons and S100 beta is a known marker for enteric glia. The aim of this study was to determine the maturation of the enteric nervous system (ENS) in normoganglionic (Ng) bowel from rats with total colonic aganglionosis (TCA). Ng ileum was obtained from TCA rats (spotting lethal: mutant, sl/sl: n = 15) at 10, 19, and 24 days of age (n = 5 for each age). Normal control Ng ileum was obtained from wild type littermates (+/+, n = 25) at 10, 19, 24, 30, and 60 days of age (n = 5 for each age). Specimens were studied with immunohistochemistry for PSA-NCAM and S100 beta. On H-E staining, normal mature ganglion cells (GC) were identified in submucus and myenteric plexuses in all specimens from TCA rats and normal controls. For PSA-NCAM, submucus and myenteric GC in control ileum were strongly positive at 10 days old, weakly positive at 19 days old, and did not stain from 24 days old and after. However, in all ileum specimens from TCA rats, both submucus and myenteric GC were strongly positive for PSA-NCAM regardless of age. For S100 beta, submucus and myenteric glial cells in control ileum were negative at 10 and 19 days old, but positive from 24 days old and after. However, in all ileum specimens from TCA rats, submucus and myenteric glial cells were S100 beta negative regardless of age. Our results suggest that GC in the Ng segment of TCA rats remain immature beyond an age when they should be mature.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Moore SW, Johnson G, Schneider JW (2000) Elevated tissue immunoglobulins in Hirschsprung’s disease-indication of early immunologic response. Eur J Pediatr Surg 10:106–110
Paran TS, Rolle U, Puri P (2006) Enteric nervous system and developmental abnormalities in childhood. Pediatr Surg Int 22:945–959. Review
Menezes M, Corbally M, Puri P (2006) Long-term results of bowel function after treatment for Hirschsprung’s disease: a 29-year review. Pediatr Surg Int 22:987–990
Kobayashi H, Hirakawa H, Surana R et al (1995) Intestinal neuronal dysplasia is a possible cause of persistent bowel symptoms after pull-through operation for Hirschsprung’s disease. J Pediatr Surg 30:253–257
Heij HA, de Vries X, Bremer I et al (1995) Long-term anorectal function after Duhamel operation for Hirschsprung’s disease. J Pediatr Surg 30:430–432
Elhalaby EA, Coran AG, Blane CE et al (1995) Enterocolitis associated with Hirschsprung’s disease: a clinical-radiological characterization based on 168 patients. J Pediatr Surg 30:76–83
Farrugia MK, Alexander N, Clarke S et al (2003) Does transitional zone pull-through in Hirschsprung’s disease imply a poor prognosis? J Pediatr Surg 38:1766–1769
Seki T, Arai Y (1993) Distribution and possible roles of the highly polysialylated neural cell adhesion molecule (NCAM-H) in the developing and adult central nervous system. Neurosci Res 17:265–290
Seki T, Arai Y (1993) Highly polysialylated neural cell adhesion molecule (NCAM-H) is expressed by newly generated granule cells in the dentate gyrus of the adult rat. J Neurosci 13:2351–2358
Seki T (2002) Expression patterns of immature neuronal markers PSA-NCAM, CRMP-4 and NeuroD in the hippocampus of young adult and aged rodents. J Neurosci Res 70:327–334
Nagahama M, Ozaki T, Hama K (1985) A study of the myenteric plexus of the congenital aganglionosis rat (spotting lethal). Anat Embryol 171:285–296
Nagahama M, Tsutsui Y, Ozaki T et al (1993) Myenteric and submucosal plexuses of the congenital aganglionosis rat (spotting lethal) as revealed by scanning electron microscopy. Biol Signals 2:136–145
Rutishauser U (1998) Polysialic acid at the cell surface: biophysics in service of cell interactions and tissue plasticity. J Cell Biochem 70:304–312
Rutishauser U, Landmesser L (1996) Polysialic acid in the vertebrate nervous system: a promoter of plasticity in cell–cell interactions. Trends Neurosci 19:422–427
Bruses JL, Oka S, Rutishauser U (1995) NCAM-associated polysialic acid on ciliary ganglion neurons is regulated by polysialytransferase levels and interaction with muscle. J Neurosci 15:8310–8319
Wester T, O’Briain DS, Puri P (1999) Notable postnatal alterations in the myenteric plexus of normal human bowel. Gut 44:666–674
Seki T, Arai Y (1991) Expression of highly polysialylated NCAM in the neocortex and piriform cortex of the developing and the adult rat. Anat Embryol 184:395–401
Beat W. Schäfer Claus W. Heizmann (1996) The S100 family of EF-hand calcium-binding proteins:functions and pathology. Trends Biochem Sci 21:134–140
Gonzalez-Martinez T, Perez-Pinera P et al (2003) S-100 proteins in the human peripheral nervous system. Microscopy Res Tec 60:633–638
Osami Nada, Takashi Kawana (1988) Immunohistochemical identification of supportive cell types in the enteric nervous system of the rat colon and rectum. Cell Tissue Res 294:523–529
Hirotaka Kato, Teiji Yamamoto et al (1990) Immunocytochemical characterization of supporting cells in the enteric nervous system in Hirschsprung’s Disease. J Pediatr Surg 25:514–519
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Horigome, F., Seki, T., Kobayashi, H. et al. Developmental anomalies of the enteric nervous system in normoganglionic segments of bowel from rats with total colonic aganglionosis. Pediatr Surg Int 23, 991–995 (2007). https://doi.org/10.1007/s00383-007-1983-x
Published:
Issue Date:
DOI: https://doi.org/10.1007/s00383-007-1983-x