Abstract
There is evidence that tissue-specific stem cells reside in certain adult tissues. Their specific properties remain elusive, because they are rare in parent tissues and heterogeneous; furthermore, technical difficulties have been encountered in their identification and the characterization of their progeny. The aim of this study was to isolate stem/progenitor cells from the human bladder. We have devised a method for isolating stem/progenitor cells from the human bladder. This is based on the enzymatic digestion of fresh surgical bladder specimens, followed by culture of cells in the presence of EGF and bFGF. We also used markers that identified and finally characterized these cells. Spheroids with self-replicative potential were obtained from all bladder specimens. The isolated population contained a subset of CD34+ CD45- cells. These spheroids represent a predominant functional type of stem/progenitor cells within the human bladder. This envisage their potential use for the treatment of animal models in pediatric surgery.
References
Kuehnle I, Goodell MA (2002) The therapeutic potential of stem cells from adults. BMJ 325:372–376
Korbling E, Estrov Z (2003) Adult stem cells for tissue repair. A new therapeutic concept? N Engl J Med 349:570–582
Blau HM, Brazelton TR, Weimann JM (2001) The evolving concept of a stem cell: entity or function? Cell 105:829–841
Weissman IL (2000) Stem cells: units of development, units of regeneration and units in evolution. Cell 100:157–168
Goodell MA, Brose K, Paradis G, Conner AS, Mulligan RC (1996) Isolation and functional properties of murine hematopoietic stem-cells that are replicating in vivo. J Exp Med 183:1797–1806
Shih CC, Weng Y, Mamelak A, LeBon T, Hu Mc, Forman SJ (2001) Identification of a candidate human neurohematopoietic stem-cell population. Blood 98:2412–2422
Gritti A, Bonfanti L, Doetsch F, Caille I, Alvarez-Buylla A, Lim DA, Galli R,Verdugo JM, Herrera DG, Vescovi AL (2002) Multipotent neural stem cells reside into the rostral extension and olfactory bulb of adult rodents. J Neurosci 22:437–445
Galli R, Gritti A, Bonfanti L, Vescovi AL (2003) Neural stem cells. An overview. Circ Res 92:598–608
Alessandri G, Pagano S, Bez A, Benetti A, Pozzi S, Iannolo G, Baronio M, Invernici G, Caruso A, Muneretta C, Bisleri G, Parati E (2004) Isolation and culture of human muscle-derived stem cells able to differentiate into myogenic and neurogenic cell lineages. Lancet 364:1872–83
Messina E, De Angelis L, Frati G, Morrone S, Chimenti S, Fiordaliso F, Salio M, Battaglia M, Latronico MVG, Coletta M, Vivarelli E, Frati L, Cossu G, Giacomello A (2004) Isolation and expansion of adult cardiac stem cells from human and murine heart. Circ Res 95:911–921
Pluchino S, Quattrini A, Brambilla E, Gritti A, Salani G, Dina G, Galli R, Del Carro U, Amadio S, Bergami A, Furlan R, Comi G, Vescovi AL, Martino G (2003) Injection of adult neurospheres induces recovery in a chronic model of multiple sclerosis. Nature 422:688–694
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This work was supported by the Italian Ministry of Health.
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Fierabracci, A., Caione, P., Giovine, M.D. et al. Identification and characterization of adult stem/progenitor cells in the human bladder (bladder spheroids): perspectives of application in pediatric surgery. Pediatr Surg Int 23, 837–839 (2007). https://doi.org/10.1007/s00383-007-1906-x
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DOI: https://doi.org/10.1007/s00383-007-1906-x