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Upregulation of mir-221 and mir-222 in atypical teratoid/rhabdoid tumors: potential therapeutic targets

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Abstract

Purpose

The aim of this study is to search for new therapeutic targets for atypical teratoid–rhabdoid tumors (ATRT).

Methods

To achieve this, we compared the expression of 365 microRNAs among ATRT, medulloblastomas, and normal brain.

Results

MiR-221 and miR-222 were within the top differentially expressed microRNAs. The deregulated expression of miR221/222 was demonstrated to inhibit the expression of the tumor suppressor and inhibitor of cell cycle p27Kip1. Here, we demonstrated the negative regulation of p27Kip1 by miR-221/222 in ATRT using microarray, real-time reverse transcriptase polymerase chain reaction, and immunohistochemistry.

Conclusion

As anti-miR therapy was recently proposed as an alternative treatment for cancer, these findings suggest that anti-miR-221/222 therapy might have therapeutic potential in ATRT.

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Acknowledgments

This project was supported by the Dr. Ralph and Marian C. Falk Medical Research Trust, the Rally Foundation for Childhood Cancer Research, and the Maeve McNicholas Memorial Foundation.

Ethical standards

This study has been approved by the Children’s Memorial Hospital Institutional Review Board (IRB#2009-13778) and had therefore been performed in accordance with the ethical standards laid down in the 1964 Declaration of Helsinki. All persons gave their informed consent prior to their inclusion in the study.

Conflict of interest

The authors declare that they have no conflict of interest.

Author information

Authors and Affiliations

  1. Cancer Biology and Epigenomics Program, Children’s Memorial Research Center, Department of Pediatrics, Feinberg School of Medicine, Children’s Memorial Hospital,  , 2300 Children’s Plaza, Chicago, IL, 60614, USA

    Simone Treiger Sredni, Maria de Fátima Bonaldo, Fabrício Falconi Costa, Christopher Allan Hamm, Jared Marshall Bischof & Marcelo Bento Soares

  2. Department of Preventive Medicine, Feinberg School of Medicine, Northwestern University, 650 North LakeShore Drive, suite 1102, Chicago, IL, 60611, USA

    Chiang-Ching Huang

  3. Interdisciplinary Graduate Program in Genetics, University of Iowa, Iowa City, IA, 52242, USA

    Christopher Allan Hamm

  4. Division of Anatomic Pathology, Children’s Memorial Hospital, 2300 Children’s Plaza, Chicago, IL, 60614, USA

    Veena Rajaram

  5. Division of Pediatric Neurosurgery, Children’s Memorial Hospital, 2300 Children’s Plaza, Chicago, IL, 60614, USA

    Tadanori Tomita

  6. Division of Hematology/Oncology/Transplantation, Children’s Memorial Hospital, 2300 Children’s Plaza, Chicago, IL, 60614, USA

    Stewart Goldman

Authors
  1. Simone Treiger Sredni
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  2. Maria de Fátima Bonaldo
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  3. Fabrício Falconi Costa
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  4. Chiang-Ching Huang
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  5. Christopher Allan Hamm
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  6. Veena Rajaram
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  7. Tadanori Tomita
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  8. Stewart Goldman
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  9. Jared Marshall Bischof
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  10. Marcelo Bento Soares
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Corresponding author

Correspondence to Simone Treiger Sredni.

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Sredni, S.T., Bonaldo, M.d.F., Costa, F.F. et al. Upregulation of mir-221 and mir-222 in atypical teratoid/rhabdoid tumors: potential therapeutic targets. Childs Nerv Syst 26, 279–283 (2010). https://doi.org/10.1007/s00381-009-1028-y

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  • DOI: https://doi.org/10.1007/s00381-009-1028-y

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