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Caffeic acid phenethyl ester preferentially enhanced radiosensitizing and increased oxidative stress in medulloblastoma cell line

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Abstract

Objectives

Caffeic acid phenethyl ester (CAPE), an active component of propolis, was recently reported to have radiosensitizing effects on medulloblastoma (MB) cells. However, the mechanisms of radiosensitivity involved in medulloblastoma cells are still unclear. The specific aim of this study was to investigate the role of CAPE-induced oxidative stress to influence of radiosensitivity and anti-proliferative effects in medulloblastoma cells.

Materials and methods

Medulloblastoma (Daoy) cells were treated with CAPE in different concentrations and assessed for cell viability. The following were also evaluated: migratory ability, reduced glutathione (GSH) level, reactive oxygen species (ROS) level, nuclear factor-kappaB (NF-κB) activity, and apoptosis in CAPE alone, radiation alone, or radiation combined with CAPE in Daoy cells.

Results

The results indicated that CAPE inhibited the growth of Daoy cells. CAPE treatment in Daoy cells could effectively decrease glutathione reductase and significantly increase glutathione peroxidase. Radiation-activated NF-κB was reversed by CAPE pretreatment. Finally, the result of terminal deoxynucleotidyl transferase-mediated dUTP–biotin nick end labeling assay showed that CAPE treatment can enhance radiation-induced apoptosis in Daoy cells.

Conclusions

Our study demonstrated the anti-proliferative and radiosensitizing effects of CAPE on MB cells, which may be achievable through depleting GSH, increased ROS activity, and inhibiting NF-κB activity.

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Acknowledgment

This study was supported by research grants from the National Science Council (NSC-96-3111-B-075-001, 96-2628-B-010-006-MY3), Taipei Veterans General Hospital (V97E1-008, V97F-001), Yen-Tjing-Ling Medical Foundation, Taipei City Hospital (96001-62-014, 96001-62-018, 96002-62-092), and National Yang-Ming University (Ministry of Education, Aim for the Top University Plan), Taiwan.

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Authors and Affiliations

  1. Institute of Clinical Medicine, National Yang-Ming University, Taipei, Taiwan

    Yi-Yen Lee, Chung-Lan Kao & Shih-Hwa Chiou

  2. Institute of Anatomy and Cell Biology, National Yang-Ming University, Taipei, Taiwan

    Ping-Hsing Tsai & Hung-Hai Ku

  3. Institute of Traditional Medicine, National Yang-Ming University, Taipei, Taiwan

    Tung-Hu Tsai

  4. Department of Physical Medicine and Rehabilitation, The Neurological Institute, Taipei Veterans General Hospital, Taipei, Taiwan

    Chung-Lan Kao

  5. Department of Education and Research, The Neurological Institute, Taipei Veterans General Hospital, Taipei, Taiwan

    Ping-Hsing Tsai & Shih-Hwa Chiou

  6. Division of Pediatric Neurosurgery, The Neurological Institute, Taipei Veterans General Hospital, Taipei, Taiwan

    Tai-Tong Wong

  7. Department of Pediatric, Taipei City Hospital, Renai Branch, Taipei, Taiwan

    Yi-Yen Lee & Wei-Fong Wu

  8. Department of Education and Research, Taipei City Hospital, Renai Branch, Taipei, Taiwan

    Tung-Hu Tsai

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  1. Yi-Yen Lee
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Correspondence to Hung-Hai Ku.

Additional information

Y.-Y. Lee and C.-L. Kao contributed equally to this article.

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Lee, YY., Kao, CL., Tsai, PH. et al. Caffeic acid phenethyl ester preferentially enhanced radiosensitizing and increased oxidative stress in medulloblastoma cell line. Childs Nerv Syst 24, 987–994 (2008). https://doi.org/10.1007/s00381-008-0636-2

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  • DOI: https://doi.org/10.1007/s00381-008-0636-2

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