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Transport stress induces apoptosis in rat myocardial tissue via activation of the mitogen-activated protein kinase signaling pathways

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Abstract

The present study aimed to elucidate the mechanism of myocardial damage induced by simulated transport stress. Sprague–Dawley rats were subjected to 35 °C and 60 rpm (0.1×g rcf) on a constant temperature shaker. The blood samples were prepared for detection of epinephrine (E), norepinephrine (NE), atrial natriuretic peptide (ANP), brain natriuretic peptide (BNP) and serum cardiac troponin T (cTNT); myocardium samples were prepared for morphological examination and signaling protein quantitative. The result showed that plasma norepinephrine (NE) and epinephrine (E) concentrations increased in all stressed groups (P < 0.01). Levels of serum cardiac troponin T (cTNT) were elevated in both the S2d (P < 0.05) and S3d groups (P < 0.01). The concentration of plasma BNP was increased significantly in S3d group (P < 0.05); the difference in ANP was not remarkable. Morphological observation demonstrated obvious microstructure and ultrastructure damage after simulated transport stress. There was also a significant increase in the number of TUNEL-positive cardiomyocytes in stressed hearts. Western blot analysis found that the mitogen-activated protein kinase (MAPK) pathways were activated by strengthening phosphorylation of ASK-1, JNK, P38 and ERK in rat myocardial tissue after simulated transport stress (P < 0.05, P < 0.01). In addition, the ratio of pro-apoptotic Bax and anti-apoptotic Bcl-2 proteins was increased in stressed rats (P < 0.01), and the amount of cleaved-caspase3 increased in all stressed rats (P < 0.01). The expression of cleaved-caspase9 protein was also elevated in S2d and S3d groups (P < 0.01). Consequently simulated transport stress induced obvious myocardial damage, which may be attributed to the activation of caspase 9-mediated mitochondrial apoptotic pathway and MAPK pathways.

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Acknowledgments

This work was supported by grants from Public Service Sectors Agriculture Research Projects (No. 201003060-9/10). We are thankful for the help from the members of CAU-BUA TCVM teaching and research team.

Conflict of interest

The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. CAU-BUA TCVM Teaching and Researching Team, College of Veterinary Medicine, China Agricultural University (CAU), No. 2 West Yuanmingyuan Road, Beijing, 100193, People’s Republic of China

    Changrong Wan, Yuping Chen, Peng Yin, Dandan Han, Xiaolong Xu, Shasha He, Mingjiang Liu & Jianqin Xu

  2. College of Animal Science and Technology, Beijing University of Agriculture (BUA), No. 7 Beinong Road, Beijing, 102206, People’s Republic of China

    Xiaolin Hou & Fenghua Liu

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  1. Changrong Wan
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  2. Yuping Chen
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Correspondence to Jianqin Xu.

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Wan, C., Chen, Y., Yin, P. et al. Transport stress induces apoptosis in rat myocardial tissue via activation of the mitogen-activated protein kinase signaling pathways. Heart Vessels 31, 212–221 (2016). https://doi.org/10.1007/s00380-014-0607-3

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  • DOI: https://doi.org/10.1007/s00380-014-0607-3

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