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Effect of fulvic acids on lead-induced oxidative stress to metal sensitive Vicia faba L. plant

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Abstract

Lead (Pb) is a ubiquitous environmental pollutant capable to induce various morphological, physiological, and biochemical functions in plants. Only few publications focus on the influence of Pb speciation both on its phytoavailability and phytotoxicity. Therefore, Pb toxicity (in terms of lipid peroxidation, hydrogen peroxide induction, and photosynthetic pigments contents) was studied in Vicia faba plants in relation with Pb uptake and speciation. V. faba seedlings were exposed to Pb supplied as Pb(NO3)2 or complexed by two fulvic acids (FAs), i.e. Suwannee River fulvic acid (SRFA) and Elliott Soil fulvic acid (ESFA), for 1, 12, and 24 h under controlled hydroponic conditions. For both FAs, Pb uptake and translocation by Vicia faba increased at low level (5 mg l−1), whereas decreased at high level of application (25 mg l−1). Despite the increased Pb uptake with FAs at low concentrations, there was no influence on the Pb toxicity to the plants. However, at high concentrations, FAs reduced Pb toxicity by reducing its uptake. These results highlighted the role of the dilution factor for FAs reactivity in relation with structure; SRFA was more effective than ESFA in reducing Pb uptake and alleviating Pb toxicity to V. faba due to comparatively strong binding affinity for the heavy metal.

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Abbreviations

Chl-a:

Chlorophyll a

Chl-b:

Chlorophyll b

EDTA:

Ethylenediaminetetraacetic acid

ESFA:

Elliott Soil fulvic acid

FAs:

Fulvic acids

HSs:

Humic substances

PCs:

Phytochelatins

ROS:

Reactive oxygen species

V. faba :

Vicia faba

SRFA:

Suwannee River fulvic acid

TBARS:

Thiobarbituric acid reactive substances

WHAM VI:

Windermere humic aqueous model VI

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Acknowledgments

The authors would like to thank the Higher Education Commission of Pakistan (www.hec.gov.pk) and the French Society for Export of Educative Resources (SFERE, http://www.sfere.fr/) for the scholarship granted to M. Shahid. The experimental work was funded by the “French Ministry of Higher Education and Research” (MESR, enseignementsup-recherche.gouv.fr) and a national project EC2CO-CYTRIX. We thank these organizations for their support which has enabled the realization of this work.

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Authors and Affiliations

  1. Université de Toulouse–INP-ENSAT, Avenue de l’Agrobiopôle, 31326, Castanet-Tolosan, France

    Muhammad Shahid, Camille Dumat, Jérôme Silvestre & Eric Pinelli

  2. UMR 5245 CNRS-INP-UPS; EcoLab (Laboratoire d’écologie fonctionnelle), 31326, Castanet-Tolosan, France

    Muhammad Shahid, Camille Dumat, Jérôme Silvestre & Eric Pinelli

  3. Department of Energy and Environmental Engineering, COMSATS Institute of Information Technology, Vehari, Pakistan

    Muhammad Shahid

  4. EcoLab (Laboratoire d’écologie fonctionnelle); INP-ENSAT, Avenue de l’Agrobiopôle, BP 32607, Auzeville tolosane, 31326, Castanet-Tolosan, France

    Camille Dumat

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  1. Muhammad Shahid
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  2. Camille Dumat
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Correspondence to Camille Dumat.

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Shahid, M., Dumat, C., Silvestre, J. et al. Effect of fulvic acids on lead-induced oxidative stress to metal sensitive Vicia faba L. plant. Biol Fertil Soils 48, 689–697 (2012). https://doi.org/10.1007/s00374-012-0662-9

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  • DOI: https://doi.org/10.1007/s00374-012-0662-9

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